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Stable Enzyme Solutions and Method of Manufacturing

a technology of stable enzymes and solutions, applied in the field of liquid compositions, can solve the problems of well-known storage stability problems, and achieve the effect of improving enzyme stability

Active Publication Date: 2020-06-11
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The addition of soluble salts significantly improves the storage stability of enzymes by maintaining enzyme activity, with residual activity extending up to 10 times longer under stressed conditions, such as elevated temperatures, compared to compositions without the booster.

Problems solved by technology

Storage stability problems are well known in enzyme containing liquids such as enzyme containing liquid detergents.

Method used

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  • Stable Enzyme Solutions and Method of Manufacturing
  • Stable Enzyme Solutions and Method of Manufacturing

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0121]Storage Stability Trial

[0122]Detergent Base:

[0123]55 g anion tenside Na-LAS

[0124]105 g anion tenside Surfac LC70

[0125]25 g nonionic tenside Neodol 25-3

[0126]30 g nonionic tenside Neodol 25-7

[0127]40 g NaCO3

[0128]33 g SXS (Sodium xylenesulfonate 40% WT solution in water)

[0129]17 g citrate-monohydrate

[0130]10 g STS (Sodium toluene sulfonate)

[0131]10 g ethanol

[0132]pH adjusted to pH 9 (NaOH)

[0133]Water ad 1000 g

[0134]pH 9

[0135]The detergent base was diluted 1:1.5 water.

[0136]The amount of salt added was 3% salt by weight based on the diluted detergent Base.

[0137]The protease was added in an amount of 0.173 KNPU-S / g, specific activity of 395 u / g.

[0138]4-FPBA was added in amounts of 0.17 mg / g of diluted detergent base+salt.

[0139]The storage conditions were four weeks storage at 40° C. was selected.

Residual activitySalt tested(4 weeks at 40° C.)Cat ionAnionMagnesium Chloride79%MgClMagnesium Nitrate55%MgNO3Armonium Chloride49%NH4ClArmonium Sulfate43%NH4SO4Armonium Nitrate41%NH4NO3Ma...

example 2

[0141]Storage Stability Trial

[0142]Detergent Base:

[0143]55 g anion tenside Na-LAS

[0144]105 g anion tenside Surfac LC70

[0145]25 g nonionic tenside Neodol 25-3

[0146]30 g nonionic tenside Neodol 25-7

[0147]40 g NaCO3

[0148]33 g SXS (Sodium xylenesulfonate 40% WT solution in water)

[0149]17 g citrate-monohydrate

[0150]10 g STS (Sodium toluene sulfonate)

[0151]10 g ethanol

[0152]pH adjusted to pH 9 (NaOH)

[0153]Water ad 1000 g

[0154]The detergent base was diluted 1:1.5 water.

[0155]The amount of salt added was 3% salt by weight based on the detergent.

[0156]The protease was added in an amount of 0.173 KNPU-S / g, specific activity of 395 u / g.

[0157]4-FPBA was added in amounts of 0.17 mg / g of detergent+salt.

[0158]The storage conditions were two weeks storage at 40° C. was selected.

Residual activityCatSalt tested(2 weeks at 40° C.)ionAnionZink Chloride102% ZnClZink Sulfate88%ZnSO4No Salt33%. . .. . .

[0159]Both zinc salts show a significant improvement in stability.

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Abstract

The invention relates to the stabilization during storage of enzymes comprised in liquid detergent compositions.

Description

FIELD OF INVENTION[0001]The present disclosure relates to a liquid composition comprising or including an enzyme, an inhibitor and an inhibitor booster.BACKGROUND[0002]Storage stability problems are well known in enzyme containing liquids such as enzyme containing liquid detergents. This is especially true in protease containing liquid detergents.[0003]The prior art has dealt extensively with improving the storage stability, for example by adding a protease inhibitor.[0004]Boric acid and boronic acids are known to reversibly inhibit proteolytic enzymes. A discussion of the inhibition of one serine protease, subtilisin, by boronic acid is provided in Molecular & Cellular Biochemistry 51, 1983, pp. 5-32.[0005]Boronic acids have very different capacities as subtilisin inhibitors. Boronic acids containing only alkyl groups such as methyl, butyl or 2-cyclohexylethyl are poor inhibitors with methylboronic acid as the poorest inhibitor, whereas boronic acids bearing aromatic groups such as...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C11D3/386C11D3/04C11D3/16C11D3/20
CPCC11D3/166C11D3/38663C11D3/2075C11D3/046C11D3/048
Inventor ANDERSEN, KIM BRUNO
Owner NOVOZYMES AS