Diagnostic scanning microscope for information-enriched qualitative histopathology

a scanning microscope and information-enriched technology, applied in the field of light optical microscopy, can solve the problems of increasing resolution, corresponding loss of field, and under-utilization of prior-art mechanized procedures by the profession, and achieves the effect of effective continuous linear coverage and highly advantageous increase in imaging speed

Inactive Publication Date: 2011-02-08
DMETRIX INC
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Problems solved by technology

As a result, these prior-art mechanized procedures are often under-utilized by the profession.
Therefore, any increase in resolution is accompanied by a corresponding loss of field of view, which greatly affects the system's capability for rapid imaging of useful-size tissue samples.
Because of the difficulty involved in aligning images of adjacent portions of the sample, the resulting composite images of overlapping features are often sufficiently misaligned to become reliable as a source of optical density information.
The stage manipulation and the attendant time required to image an object under high magnification is particularly troublesome in pathology analysis because the diagnostic information in the tissue may be located in only a small portion of the tissue that is being imaged.

Method used

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  • Diagnostic scanning microscope for information-enriched qualitative histopathology
  • Diagnostic scanning microscope for information-enriched qualitative histopathology
  • Diagnostic scanning microscope for information-enriched qualitative histopathology

Examples

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example 1

[0066]FIG. 25 shows a histopathologic section of urothelium taken from a region judged to be histologically normal at some distance from a papillary carcinoma lesion in a bladder. Nine nuclei in the image (identified by highlighted boundaries in the figure) were selected as suitable for analysis for their in-focus position. Upon digital analysis, two of these nuclei showed a nuclear signature resembling that of papillary carcinoma, even though visually the section was normal. Utilizing a computer graphic marker in the form of an outlining box, the diagnostic information is revealed directly on the image, thereby providing a visual, quantitative diagnostic clue not otherwise detectable from the image.

example 2

[0067]A tissue sample from a prostate gland duct was sectioned, fixed and stained with hematoxylin / eosin. The resulting slide was scanned with a high numerical aperture microscope objective to produce an image of the sample, as shown in FIG. 26. The image exhibits all criteria for a normal gland, such as a single-layered epithelium and a basal cell layer without gaps. However, a numeric analysis of the chromatin pattern of the nuclei revealed an overall signature known to be typical of a “normal appearing gland” in the vicinity of a prostatic intraepithelial neoplasia (PIN) lesion. FIG. 27 illustrates the signature of the imaged sample (seen in the middle of the figure) in comparison with the signatures of normal tissue (above) and cancerous tissue (below).

[0068]According to one approach, the signature profile of the epithelium is displayed superimposed on the image of the stained sample to reveal the diagnostic clue that is not otherwise visually perceptible, as illustrated in FIG....

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Abstract

A microscope array with staggered rows of magnifying imaging systems is used to scan a biological tissue sample in a single linear pass to produce an image and corresponding optical-density data. A conventional computerized algorithm is used to identify, isolate and produce segmented images of nuclei contained in the image. The OD values corresponding to nuclear chromatin are used to identify numerical patterns known to have statistical significance in relation to the health condition of the biological tissue. These patterns are analyzed to detect pre-neoplastic changes in histologically normal-appearing tissue that suggest a risk for the development of a pre-malignant and a potentially malignant lesion. This information is then converted to a visually perceptible form incorporated into the image of the tissue sample and is displayed for qualitative analysis by a pathologist.

Description

RELATED APPLICATIONS[0001]This application is a continuation-in-part application of U.S. Ser. No. 10 / 637,486, filed Aug. 11, 2003 now U.S. Pat. No. 7,184,610 B2, which is based on PCT / US02 / 08286, filed Mar. 19, 2002, and claims the benefit of priority of U.S. Provisional Application No. 60 / 276,498, filed Mar. 19, 2001, under 35 U.S.C. Sect. 119, and is also a continuation-in-part application of U.S. Ser. No. 10 / 602,756, filed Jun. 24, 2003 now U.S. Pat. No. 7,343,033 B2.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]This invention is related in general to the field of light optical microscopy. In particular, it relates to a method and apparatus for conducting diagnostic testing of biological tissue with an array scanning microscope.[0004]2. Description of the Related Art[0005]Changes in the cellular structure of tissue are used to detect pathologic changes, to assess the progress of precancerous conditions, and to detect cancer. A tissue sample removed from a patien...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): G06K9/00A61B5/05G21K7/00
CPCG06K9/00127G02B21/002G02B21/365G06V20/69
Inventor BARTELS, PETER H.
Owner DMETRIX INC
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