The invention provides a composite forming auxiliary
reagent for assisting living tissue shaping and a living tissue explant
slice preparation method. The preparation method comprises the following steps: dissolving 4-15 parts by
mass of methacrylated polyether F127 diacrylate F127DA, 2.5-5 parts by
mass of a GelMA
gelatin aqueous solution, 4-6 parts by
mass of
gelatin, 2.5-5 parts by mass of AlgMA
sodium alginate hydrogel and 0.20-0.30 part by mass of an LAP
photoinitiator in 100 parts by volume of a PBS
buffer solution, and then completely immersing a tissue block into a composite forming auxiliary
reagent, irradiating by using a UV
light source to obtain a tissue hydrogel block, and
slicing to obtain a living tissue explant slice; according to the composite forming auxiliary
reagent, a tissue hydrogel block formed by wrapping a tissue block has proper strength and
toughness, so that the whole structure can be ensured to have certain
hardness and
toughness, the tissue block is not easy to deform during
cutting, and extra damage and even death of cells in the tissue caused by excessive strength under
extrusion are also avoided; and gel fragmentation caused by
cutting can be avoided, so that living tissue explant slices with uniform thickness are obtained.