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Method for triptolide asexually

A technology of asexual reproduction and Tripterygium wilfordii, applied in the biological field, can solve problems such as the induction, propagation and transplantation of tripterygium wilfordii in vitro culture cluster bud induction, multiplication and transplanting research and patent reports, etc.

Inactive Publication Date: 2007-08-29
上海延农生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no research and patent reports on the induction, propagation and transplantation of Tripterygium wilfordii in vitro

Method used

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  • Method for triptolide asexually

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Induction of clustered buds of Tripterygium wilfordii

[0068] Take Tripterygium wilfordii germinated in the same year as the initial donor material, sterilize with 4% chlorinated spirit for 10 minutes, rinse with sterile water for several times, and after absorbing the water, take young shoots or stems with axillary buds as explants , inoculate in MS medium containing 6-BA 0.2mg / L, KT 0.2mg / L, add sucrose 30g / L, agar 6g / L, adjust pH to 5.6, and induce clustered buds, 22~25℃, 10-12 hours of light per day, after 30-40 days of culture, 95% of the explants can induce clustered buds.

Embodiment 2

[0069] Embodiment 2 Proliferation of Tripterygium wilfordii clustered buds

[0070] After the elongation of the induced clustered buds, take the section of each new branch with top / axillary buds, and transfer it to the propagation medium, the basic medium is MS, and add cytokinin 6-BA0.1mg / L, KT0 .1mg / L, sucrose 30g / L, agar 6g / L, adjust pH to 5.6, carry out the multiplication of clustered buds, culture temperature 22~25℃, light for 10~12 hours every day, after cultivating for 30~40 days, its proliferation coefficient can be up to 6.0 or above.

Embodiment 3

[0071] Example 3 The strong seedlings of Tripterygium wilfordii clustered with buds

[0072] Add hydrolyzed casein 500mg / L to the added MS medium, add cytokinin 6-BA0.1mg / L, KT0.1mg / L, sucrose 30g / L, agar 6g / L, adjust the pH to 5.6, 22 ~25°C, 10-12 hours of light per day, culture for 20-30 days, to obtain clustered buds with dark green color, strong stems and vigorous growth.

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Abstract

The invention relates to a method for the asexual propagation of Tripterygium wilfordii, which comprises: inducing the clump buds, breeding, culturing the sprout and replanting etc. The method can protect the resource of wild Tripterygium wilfordii, providing a new way of sustainable supplying the Tripterygium wilfordii.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, the present invention relates to a method for asexual propagation of Tripterygium wilfordii, in particular to the steps of induction, multiplication, seedling strengthening, rooting and transplanting of clustered buds. Background technique [0002] Tripterygum wilfordii Hook f. is a plant of the genus Tripterygum wilfordii Hook f. Tripterygium wilfordii, as a unique Chinese medicine, is gradually going to the world with its strong efficacy and wide application, and it is expected to become a good medicine for overcoming various chronic diseases. [0003] Tripterygium wilfordii is a perennial woody vine, which grows slowly and is unrestrictedly exploited in large quantities, which has caused serious damage to wild resources. In recent years, relevant domestic units have adopted seed and division propagation methods for artificial planting, but its reproduction coefficient is low, and i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/00
Inventor 黄剑华王亦菲陆瑞菊孙月芳周润梅俞庆全
Owner 上海延农生物工程有限公司
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