Physiological microspecies monogenically identifying system for rice blast germ and its construction process

A technology for physiological races and rice blast fungus, which is applied in the fields of plant genetic improvement, botanical equipment and methods, applications, etc., and can solve the problems of lack of true single-gene characteristic verification methods, such as the strength of single-gene system race discrimination.

Inactive Publication Date: 2007-12-12
CROP BREEDING & CULTIVATING INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0005] To sum up, at present, there is no real single-gene identification system that can be used uniformly in the world. The technical obstacle is the lack of

Method used

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  • Physiological microspecies monogenically identifying system for rice blast germ and its construction process

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: the cultivation of 6 single gene near isogenic lines

[0037] In 1987, LTH was used as the female parent, and the differential varieties Caodi (Pi-k, Pi-sh) and Meiyuming (Pi-k) with known disease resistance genes in Kiyosawa, Japan m , Pi-sh), K1 (Pi-ta, Pi-x), Pi4 (Pi-ta 2 , Pi-sh), K60 (Pi-k p , Pi-sh) and BL1 (Pi-b, Pi-sh) and other donor parents were crossed, and F was obtained in the spring of 1988 1 seed. In the summer of 1988, identification of F 1 Plants, select disease-resistant individuals as female parents, and backcross with LHT to obtain BC 1 f 1 seed. Since the winter of 1988, continuous backcrossing and resistance identification. Received BC in 1991 6 f 1 seed. Inbred in 1992 to obtain BC 6 f 2 seed. Plant BC of each combination in the same year 6 f 2 Seeds, harvested by individual plants BC 6 f 3 . Inoculation identification in 1993, screening out BC 6 f 3 There are 72 disease-resistant strains. In 1994, it was inocula...

Embodiment 2

[0038] Embodiment 2: the verification of the gene composition analysis and single gene characteristic of 6 near isogenic lines (NILs) of the present invention

[0039] The resistance of 6 donor parents and 6 near-isogenic lines to Philippine isolates is as follows: 1. The donor parents show disease resistance, and the near-isogenic lines show disease resistance; 2. The donor parents show disease resistance 3. The donor parent showed a susceptible response, and the near-isogenic line showed a susceptible response. In all the combinations, the donor parents were never found to show disease-response, and the near-isogenic line showed disease-resistant response. It can be seen from the results that the six near-isogenic lines in China only express Pi-k, Pi-k m , Pi-ta, Pi-ta 2 、Pi-k p , and Pi-b resistance, while the 6 donor parents also showed the role of Pi-sh, or other disease resistance genes in addition to the role of these genes, so the resistance spectrum of the donor pa...

Embodiment 3

[0041] Example 3: Comparison with Donor Discrimination

[0042] Six near-isogenic lines and their six donor parents were inoculated with 42 strains from the Philippines, 25 strains from Japan and 100 strains from the japonica region of northern China. The results of race discrimination comparison are as follows:

[0043] Table 5. Comparison results of race discrimination

[0044] vaccination site

[0045] This result shows that the 6 near-isogenic lines and 6 donor parents of the present invention have the same physiological race discrimination ability for strain division in northern China and Japan. However, the ability of the 6 near-isogenic lines to identify the races of Magnaporthe grisea in the indica region was significantly higher than that of their donor parents.

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Abstract

The present invention aims at provides one kind of physiological microspecies monogenically identifying system for rice blast germ with high identification capacity and wide applicable range and its culture method. The system is one monogenic near isogenic line cross bred with LTH as one local japonica rice variety without major disease resistant gene as recurrent parent and through cross breeding and backcross to introduce known disease resistant gene into LTH. The system constituting process includes cross breeding and backcross with LTH as recurrent parent to introduce known disease resistant gene into LTH and to obtain the near isogenic line; identifying and confirming the monogenic characteristic of the near isogenic line; and deciding the physiological microspecies discriminability of the near isogenic line and constituting new discrimination system. The monogenic near isogenic line is suitable for various rice producing areas in the world.

Description

technical field [0001] The invention relates to a method for constructing a identification system for physiological races of rice blast fungus. Background technique [0002] The history of more than 80 years of research on the physiological race of Magnaporthe grisea has had more than 10 sets of identification systems so far for the research on the physiological race of Magnaporthe grisea (Ling Zhongzhuan et al.: Rice Blast Resistance Breeding, Fujian Science Press, 1990, pp:55-59). These identification systems are divided into three categories: the first category refers to the identification systems used by various countries from the early 1960s to the mid-1970s. The identification systems in this period were selected by geneticists and pathologists based on their own experience. Yes, they all include indica and japonica varieties, and the genetic background of each variety is different; there is no analysis of the disease resistance genes of the varieties, the composition...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 凌忠专王久林雷财林潘庆华蒋琬如
Owner CROP BREEDING & CULTIVATING INST CHINESE ACAD OF AGRI SCI
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