Agricultural waste deodorizing bacterial preparation and its production process

A technology for agricultural waste and deodorant fungicide, applied in the field of microorganisms, can solve problems such as unsatisfactory effects, and achieve the effects of increasing temperature, increasing the number of microorganisms, and increasing the nitrogen retention rate

Inactive Publication Date: 2008-08-27
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to effectively solve this problem, there are many deodorant bacteria products coming out at home and abroad, mainly chemical deodorant bacteria agents, mainly pH regulators, oxidants or bactericides; there are also some microbial deodorant bacteria agents, such as enzyme bacteria and EM bacterial agents, these products relieve the odor of organic waste to varying degrees, but the effect is not ideal and has not been accepted by the market

Method used

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  • Agricultural waste deodorizing bacterial preparation and its production process
  • Agricultural waste deodorizing bacterial preparation and its production process
  • Agricultural waste deodorizing bacterial preparation and its production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 A kind of agricultural waste deodorizing bacteria agent

[0036] It is composed of Bacillus megaterium, Coptidis chinensis and Trichoderma koningeni, and the mass-to-number ratio of the above three microorganisms is 0.5-1.5:0.5-1.5:0.5-1.5.

Embodiment 2

[0037] Embodiment 2 prepares the method for the deodorant agent of agricultural waste described in embodiment 1

[0038] 1. Production process flow chart (see figure 1 );

[0039] 2. Medium

[0040] 2.1 Potato agar medium: 200g of peeled potatoes, 20g of glucose, 15-20g of agar, sterilized in 15 pounds for 30 minutes.

[0041] 2.2 Gaussian starch agar medium: Potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate 10mg, soluble starch 20g, agar 20g, cold water 1000mg, pH7.2~ 7.4, 8 pounds 30min sterilization.

[0042] 2.3 Nutrient broth agar medium: peptone 10g, beef extract 3.0g, sodium chloride 10g, agar 15g, distilled water 1000ml, pH 7.0, sterilized in 15 pounds for 30 minutes.

[0043] 2.4 The carrier medium is composed of 50% zeolite, 20% bran, 14% bone meal, 14% fish meal and 2% brown sugar in mass percentage.

[0044] 3. Purchase 1 tube each of Coptis chinensis, Actinomyces commonis, and Trichoderma ...

Embodiment 3

[0047] Embodiment 3 A kind of agricultural waste deodorizing bacteria agent

[0048] It is composed of Bacillus megaterium, Coptis chinensis and Trichoderma koningen in a ratio of 1:1:1 by weight.

[0049] Experimental Example 4 The method for preparing the agricultural waste odor agent described in Example 3 is the same as that in Example 1.

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PUM

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Abstract

The agricultural waste deodorizing bacterial preparation is prepared through the following steps: the slant test tube culture, liquid seed culture and tertiary extension culture of giant bacillus, micro xanthoderma and Koningii Trichoderma to prepare three kinds of pure bacterial preparation; inoculating the three kinds of pure bacterial preparation in the equal ratio and inoculated amount of 0.2 % to carrier culture medium and culturing at water content of 55-60 % and temperature 28-34 deg.c for 7 days; and final drying the compound bacterial preparation at 35-45 deg.c and live bacteria inspection. The bacterial preparation of the present invention has obvious deodorizing effect and fast acting.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and is specifically applicable to an agricultural waste deodorizing bacterial agent for eliminating the odor of agricultural waste such as livestock and poultry manure and crop stalks and a preparation method of the product thereof. Background technique [0002] With the rapid development of modern agriculture, especially the large-scale and intensive production of animal husbandry, large-scale centralized disposal of agricultural waste such as livestock and poultry manure has resulted; Surprisingly, the proportion of organic components in agricultural waste and domestic waste is very high and the composition is complex. These organic wastes contain a large number of pathogenic microorganisms and parasite eggs, and a large amount of malodorous gases will be generated during the disposal process, mainly including ammonia, hydrogen sulfide, indole, mercaptans, sulfides, formaldehyde, acetald...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/16A61L11/00A61L101/52
Inventor 李鸣雷谷杰高华秦清军
Owner NORTHWEST A & F UNIV
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