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Use of SARS coronary virus ORF8 region

A coronavirus, ORF8 technology, applied in the field of virology and molecular epidemiology

Inactive Publication Date: 2008-10-29
SOUTH RES CENT NAT HUMAN GENE GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far there is no corresponding method

Method used

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  • Use of SARS coronary virus ORF8 region
  • Use of SARS coronary virus ORF8 region
  • Use of SARS coronary virus ORF8 region

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of SARS coronavirus genome typing detection kit

[0062] A kit is prepared which contains:

[0063] Name Sequence (5′→3′) Number Concentration

[0064] Forward primer atgaatgagc tcactttaat SEQ ID NO: 3 dry powder 2OD

[0065] Reverse primer ttaatttgtt cgtttatta SEQ ID NO: 4 dry powder 2OD

[0066] PCR reaction solution containing Taq enzyme dNTP magnesium ion PCR reaction buffer

Embodiment 2

[0068] Detection of infectivity and genome typing of SARS coronavirus

[0069] With the test kit in embodiment 1, three kinds of SARS coronavirus strains (blind method) to be tested carry out PCR amplification (please describe the detection process in a little detail), then sequence determination is carried out to the amplified product. The infectivity was determined according to whether the ORF8 region was missing.

[0070] The test results are consistent with the actual infectivity of the three SARS coronavirus strains (that is, the number of clinically infected people). In addition, the SARS coronavirus genotype determined based on the deletion is completely correct.

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Abstract

The present invention discloses the correlation between the ORF8 area and the virus evolution stage of coronavirus causing SARS. Based on this correlation, the present invention provides the method of extracorporeal determination of SARS coronavirus genetype, the method of mutating SARS coronavirus to stabilize its extracorporeal culture level, and the application of ORF8 area as medicine target in screening medicine for preventing and treating SARS.

Description

technical field [0001] The present invention relates to the fields of virology and molecular epidemiology, and more specifically relates to the application of the ORF8 region of SARS coronavirus causing severe acute respiratory syndrome virus. Background technique [0002] In 2003, my country and many countries in the world were attacked by a large-scale atypical pneumonia (Atypical pneumonias). Severe acute respiratory syndrome (Severe Acute Respiratory Syndrome, SARS) caused by atypical pneumonia has occurred in 22 countries and regions. The World Health Organization (WHO) quickly established a collaborative research and monitoring network consisting of 13 laboratories in 10 countries around the world after the outbreak. On April 16, WHO officially confirmed that the pathogen of SARS was a new type of coronavirus, which is a single-stranded RNA virus. [0003] There have been more than 8,000 cases of atypical pneumonia in the world, and the death rate in some diseased are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/18C12N7/01
CPCY02A50/30
Inventor 赵国屏林锦炎宋怀东郑焕英王升跃何剑峰任双喜鄢心革李亦学万卓越孔祥银许锐恒郝沛吴新伟顾伯伟侯金林陈竺闵军缪有刚徐军傅刚陆家海张树义王小宁
Owner SOUTH RES CENT NAT HUMAN GENE GROUP