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Method for preparing conjugated linoleic acid

A conjugated linoleic acid and seed oil technology, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low utilization rate and achieve low sales price, high yield, isomerization make up a single effect

Inactive Publication Date: 2009-03-18
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although halophytes are abundant, the utilization rate is not high, especially the use of halophyte seeds to produce conjugated linoleic acid still has a very broad space for development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of culture medium: 8g of commercially available beef extract, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, 20g of glucose, 2g of ammonium citrate, 10g of peptone, 4g of yeast extract powder, 5g of sodium acetate, dihydrogen phosphate Mix 2g of potassium and 1g of Tween 80 into a 2L beaker, add distilled water to one liter, adjust the pH value to 6.5 with sodium phosphate buffer solution, and sterilize by autoclaving for 20 minutes to obtain medium (1), which is used for the next step of seed Liquid pre-culture;

[0025] (2) Seed liquid pre-cultivation: Add 10 microliters of alfalfa seed oil and 1 ml of lactic acid bacteria Lactobacillus plantarum (bacteria number: 6026) solution into 20 ml of medium (1) with a pipette gun under aseptic conditions. Pre-cultivate in an incubator at ℃ for 12 hours as an inoculum, and use it for the next step of lactic acid bacteria fermentation isomerization;

[0026] (3) Fermentation isomerization of lactic acid...

Embodiment 2

[0029] (1) The preparation of culture medium (1): with embodiment 1;

[0030] (2) seed liquid pre-cultivation: with embodiment 1;

[0031] (3) Fermentation isomerization of lactic acid bacteria: prepare medium according to the formula of medium (1), adjust the pH value to 6.5 with sodium phosphate buffer solution, and sterilize by autoclaving for 20 minutes to obtain medium (2). Insert the inoculum and the alfalfa seed oil obtained in the (2) step into the culture medium (2) with a pipette gun, the inoculum size is 5% (v / v), and the amount of oil is 20 microliters. Cultivate in a 37°C incubator for 24 hours to obtain a fermentation broth;

[0032] (4) Extraction of conjugated linoleic acid: Same as Example 1, calculated conjugation conversion rate is 12.142%.

Embodiment 3

[0034] (1) The preparation of culture medium (1): with embodiment 1;

[0035] (2) seed liquid pre-cultivation: with embodiment 1;

[0036] (3) Fermentation isomerization of lactic acid bacteria: prepare medium according to the formula of medium (1), adjust the pH value to 7.0 with sodium phosphate buffer solution, and sterilize by autoclaving for 20 minutes to obtain medium (2). Insert the inoculum and alfalfa seed oil obtained in step (2) into the culture medium (2) with a pipette gun, the inoculum size is 5% (v / v), and the amount of oil is 10 microliters, shake well by hand Afterwards, culture in a 37°C incubator for 24 hours to obtain a fermentation broth;

[0037](4) Extraction of conjugated linoleic acid: Same as Example 1, calculated conjugation conversion rate is 20.789%.

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Abstract

The present invention is fermentation process of preparing conjugate linoleic acid with the seed of halophyte alfalfa as material. The process includes the following steps: 1. compounding culture medium, 2. pre-culturing the seed liquid via adding alfalfa seed oil and lactic acid bacteria to the culture medium, 3. fermenting to isomerizes and to obtain fermented liquid, and 4. extracting conjugate linoleic acid via dissolving the fermented liquid in isopropyl alcohol and extracting the fermented liquid with n-hexane. The preparation process of the present invention has low material cost, simple process, mild reaction condition and single isomer composition.

Description

technical field [0001] The technical scheme of the invention relates to a method for preparing conjugated linoleic acid by fermentation. Background technique [0002] In 1978, researchers at the University of Wisconsin in the United States found that boiled beef contained anti-mutation bioactive substances, namely conjugated linoleic acid (CLA). Subsequent animal experiments have found that CLA has various physiological effects such as anti-cancer, reducing body fat, lowering cholesterol levels, and preventing atherosclerosis. Natural CLA mainly exists in the milk and meat products of ruminants, and people mainly consume CLA through the consumption of animal fat and milk products. However, studies have found that the CLA content in the milk of cows grown on feed in captivity is much lower than that in milk of cows that are naturally grazing and grazing. In addition, people eat less animal fat, resulting in insufficient CLA intake. In order to meet the needs of in-depth res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/225
Inventor 齐树亭董明石玉新武洪庆王晓宇
Owner HEBEI UNIV OF TECH
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