Process for producing 1,3-propylene glycol by microorganism aerobic fermentation

A technology of aerobic fermentation and propylene glycol, applied in the direction of fermentation, etc., can solve the problems of high investment and energy consumption, achieve the effect of increasing the final concentration, reducing investment and energy consumption, and reducing equipment requirements

Active Publication Date: 2009-05-27
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The object of the present invention is to provide a method for microbial aerobic fermentation to produce 1,3-propanediol, which solves the problems of high investment in fixed equipment and high energy consumption, and improves the final PDO concentration

Method used

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  • Process for producing 1,3-propylene glycol by microorganism aerobic fermentation
  • Process for producing 1,3-propylene glycol by microorganism aerobic fermentation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] (1) Bacteria: Klebsiella pneumoniae

[0018] (2) Medium:

[0019] (A) Solid medium (g / L)

[0020] LB medium: yeast extract powder 5, peptone 10, NaCl 5, agar 15, adjust pH 7.0. For short-term preservation and activation of Klebsiella pneumoniae strains.

[0021] (B) Seed medium and fermentation medium

[0022]

[0023] *Preparation of iron solution: add FeSO per liter of water 4 ·H 2 O 5.0g, 37% concentrated hydrochloric acid 4ml.

[0024] Composition of trace element solutions

[0025]

[0026] (3) Training method:

[0027] The strains preserved in glycerol tubes were transferred to LB slant and activated at 37°C for 12 hours. Seed culture adopts aerobic culture, using a 500ml Erlenmeyer flask with a liquid volume of 100ml. The culture temperature was 37° C., and the rotation speed of the shaker was 120 rpm.

[0028] The fermentation culture uses a 5L fermenter with a liquid volume of 4L, a culture temperature of 37°C, and a pH value of 6.8. During the...

example 2

[0032] (1) Bacteria: Klebsiella pneumoniae

[0033] (2) culture medium is the same as example 1

[0034] (3) The cultivation method is the same as in Example 1, but the fermentation temperature is 40°C.

[0035] (4) The fermentation result is as follows:

[0036] Ferment for 25 hours, the concentration of 1,3-propanediol is 35g / l, and the concentration of lactic acid is 10g / l. The production intensity of 1,3-propylene glycol is 1.4g / (lh). Ferment for 48 hours, 1,3-propanediol concentration 44g / l, lactic acid 23g / l. The production intensity of 1,3-propylene glycol is 0.92g / (lh).

example 3

[0038] (1) Bacteria: Klebsiella pneumoniae

[0039] (2) culture medium is the same as example 1

[0040] (3) The cultivation method is the same as in Example 1, but the stirring speed is 250 rpm.

[0041] (4) Fermentation result:

[0042] After 48 hours of fermentation, the concentration of 1,3-propanediol was 56.5g / l, 2,3-butanediol was 7.4g / l, lactic acid was 40.7g / l, and acetic acid was 1.8g / l. The production intensity of 1,3-propylene glycol is 1.2g / (lh). Ferment for 68 hours, 1,3-propanediol concentration 60.5g / l, 2,3-butanediol 7.7g / l, lactic acid 53.4g / l, acetic acid 2g / l. The production intensity of 1,3-propylene glycol is 0.89g / (lh).

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Abstract

The present invention provides microbial aerobic fermentation process of producing 1, 3-propylene glycol, and belongs to the field of biochemical technology. Seed liquid is first added into industrial glycerin or initial fermentation culture medium of glycerin fermenting liquid with glycerin concentration of 20-50 g / L, and under fermentation condition of 30-40 deg.c, the culture medium is fermented with PDO producing Klebsiella pneumoniae or acid producing Klebsiella pneumoniae to produce PDO. During fermentation, air in 0.2-1.0 vvm is led in, the fermented matter is stirred in 50-250 rpm, glycerin and sodium hydroxide in certain amount are added to maintain certain glycerin concentration. The present invention has the advantages of lowered investment and power consumption, raised final PDO concentration, raised production strength and increased bacteria biomass.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, in particular to a method for producing 1,3-propanediol by microbial aerobic fermentation. Background technique [0002] 1,3-Propanediol (PDO) is an important chemical raw material, which can be used as an organic solvent in ink, printing and dyeing, paint, lubricant, antifreeze and other industries. The most important use of 1,3-propanediol is as a monomer for polyester and polyurethane synthesis, especially polytrimethylene terephthalate (PTT), which is polymerized with terephthalic acid, shows a ratio of 1,2- Polymers synthesized from propylene glycol, butanediol, and ethylene glycol have better performance. At present, the world consumes tens of millions of tons of polyethylene terephthalate (PET) every year, and the chemical stability and biodegradability of PTT are comparable to PET, but the pollution resistance, toughness, resilience and UV resistance, etc. more superior. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18
Inventor 刘德华孙燕刘宏娟郝健林日辉
Owner TSINGHUA UNIV
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