Unlock instant, AI-driven research and patent intelligence for your innovation.

Process for extracting cyclic adenosine monophosphate (cAMP) from Chinese date

A cyclic adenosine monophosphate and process technology, applied in the field of extraction of cyclic adenosine monophosphate, can solve the problems of high toxicity, side effects, mutagenesis, carcinogenicity, etc., and achieve the effect of low cost

Inactive Publication Date: 2009-06-10
天津市医药科学研究所 +2
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. High cost: due to the use of ribonucleic acid (RNA) as the raw material for the production of cyclic adenosine monophosphate (cAMP), ribonucleic acid (RNA) itself is very expensive, about 25,000 yuan per kilogram, and the adenine nucleotide in it is only It accounts for 1 / 4 of ribonucleic acid (RNA), so currently only a few companies in China that use ribonucleic acid (RNA) as raw materials produce cyclic adenosine monophosphate (cAMP) at least 120,000 yuan per kilogram. Foreign manufacturers such as Sigma Each kilogram of cyclic adenosine monophosphate (cAMP) is as high as 215,000 US dollars or about 1.785 million yuan
[0005] 2. High toxicity and side effects: Due to the addition of some unnatural catalysts and chemical reagents in the repeated hydrolysis process and cyclization steps of ribonucleic acid (RNA), a small amount of impurities are mixed in the final product, which enters the human body and causes certain toxicity and side effects. Some impurities even stay in the body for a long time and become carcinogenic, teratogenic, and mutagenic substances. Therefore, the cyclic adenosine monophosphate (cAMP) produced at home and abroad is only used in biochemical and molecular biology research as biochemical reagents and animal experimental treatments. Use, there is no clinical report for patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, the technique of producing cyclic adenosine monophosphate (cAMP) as raw material with Jinsixiaozao in Jinghai area is realized according to the following procedures:

[0023] Soaking: Jinsi jujube is washed, dried, and weighed after removing the core. Add 5 liters of distilled water to 500 grams, and soak in distilled water for 24-48 hours;

[0024] Homogenization: Use a tissue masher or a tissue homogenizer to mash and homogenize the above soaking liquid, for 3 minutes each time, rest for 20 minutes, and repeat 3 times. This step and subsequent steps are all at 4°C±1 Under the temperature condition of ℃;

[0025] Centrifugation: Use a high-speed refrigerated centrifuge to centrifuge the above-mentioned homogenate at a speed of 12000g for 15 minutes; take out the supernatant, and then use 2.5 liters of distilled water to homogenize according to the conditions under the homogenization item and press the centrifugation item. Conditional centrifugation, the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a process for extracting adenosine 3, 5-monophosphate (cAMP). The invention comprises the following steps: cleaning, airing and stoning jujube; steeping jujube for 24-28 h in water; treating supernatant in ion exchange column for several times; treat it with separation, purification and freeze drying to generate dry powder. Compared with existing technology, the invention has the advantages of low cost and non-toxicity.

Description

technical field [0001] The invention provides a process for extracting cyclic adenosine monophosphate (cAMP) from jujube Background technique [0002] At present, cyclic adenosine monophosphate (cAMP) produced by manufacturers at home and abroad are artificially synthesized products, and its raw material is ribonucleic acid (RNA), which is composed of adenine nucleotides, guanine nucleotides, uracil nucleotides, cellular Composed of pyrimidine nucleotides, the molecule of adenine nucleotides contains adenine, ribose and phosphate. The molecule of cyclic adenosine monophosphate (cAMP) also contains adenine, ribose and phosphoric acid. The phosphate group and the 3' and 5' hydroxyl groups of ribose form two ester bonds to form a ring structure, so it is named cyclic phosphoric acid. Adenosine (cAMP). Because ribonucleic acid (RNA) contains the raw material of synthesizing cyclic adenosine monophosphate (cAMP), therefore domestic and foreign manufacturers all adopt ribonuclei...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/20C07H1/08
Inventor 李明润于文才杜恩祥高向耘林木强刘志忠
Owner 天津市医药科学研究所