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Microorganism metabolism control of actinoplanes species

A technology of microbial metabolism and swimming actinomycetes, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of shortened product synthesis stages, high nutrient concentration, and reduced output of metabolites, and achieve easy industrialization The effect of high production and fermentation level and low production cost

Active Publication Date: 2009-07-29
HUADONG MEDICINE +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This feeding method has great disadvantages. Because it is a one-time supplement of carbon source, nitrogen source and other necessary substrates, the concentration of nutrients will be too high in a short period of time, which will cause the bacteria to multiply mycelia in large quantities. The direction of the body is developed, so that the nutrients are mainly consumed in the growth of the mycelia, the synthesis stage of the product is shortened, and the output of the final metabolite is reduced.
After improvement, the metering tank feeding method can also be used for feeding. Although this method can eliminate the suppression phenomenon caused by the excessive concentration of nutrients produced by the one-time feeding of a large amount of nutrients in periodic feeding, it does not affect the mycelium growth. There are still certain defects in the feeding method, that is, the same amount of substrate is added at a constant rate throughout the feeding cycle, and the actual consumption of the bacteria in each stage of the metabolic process is ignored. Compared with periodic feeding, metering is used. Although the fermentation unit of tank-fed acarbose has improved, it is not satisfactory

Method used

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  • Microorganism metabolism control of actinoplanes species

Examples

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Effect test

Embodiment 1

[0019] Periodic feeding: connect the feeding tank and the fermentation tank through pipelines. After the feeding tank is sterilized, sterilize the feeding pipeline. After completion, press 4 tons of material into the fermentation tank through sterile air to complete the feeding. The whole fermentation process then needs 4 times of this feeding process, and the total feeding amount is 16 tons. Put it in the tank and measure the fermentation unit: 3430μg / ml.

[0020] Continuous automatic variable speed feeding: install a distributed control system on the computer, connect the feeding tank and the fermentation tank through pipelines, and install an automatic diaphragm valve in the middle. After the feeding tank is sterilized, start the automatic feeding device to sterilize the feeding pipeline. After the completion, use sterile air to hydraulically pass the material, and pass the automatic diaphragm valve (the computer controls the opening and closing time of the valve according ...

Embodiment 2

[0024] A distributed control system is installed on the computer, the feed tank and the fermenter are connected through pipelines, and an automatic diaphragm valve is installed in the middle. After the feeding tank is sterilized, start the automatic feeding device to sterilize the feeding pipeline. After the completion, use sterile air to hydraulically pass the material, and pass the automatic diaphragm valve (the computer controls the opening and closing time of the valve according to the set feeding amount) ) into the fermenter.

[0025] Continuous automatic constant-speed feeding: From about 40 hours in the early stage of fermentation to about 144 hours in the middle and late stages of fermentation, 2.5L of feed liquid is added per minute, and the total amount of feeding is 15 tons. Put the tank, and measure the fermentation unit 4634μg / ml.

[0026] Continuous automatic variable speed feeding: Feed feeding starts at 44 hours after fermentation, and feeds 3.5L of feed liqui...

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Abstract

The invention discloses a method for preparing high-unit acarbose by regulating the microbial metabolism of actinomycete Actinoplanes species. The invention adopts a computer automatic variable speed feeding method to start feeding after 40-48 hours of microbial fermentation, and feed 3 times per minute. -4L feed liquid; continue until 84-92 hours to adjust the feeding amount to 1-2L feed liquid per minute. The acarbose prepared by the method of the invention has a high fermentation level, saves raw materials, has low production cost, does not need to modify original equipment, and is easy for industrialized production.

Description

technical field [0001] The invention relates to the field of biochemical industry, and more specifically relates to a method for preparing high-unit acarbose by regulating microbial metabolism. Background technique [0002] Acarbose, chemical name: O-4,6-dideoxy-4-[(1S,4R,5S,6S)4,5,6-trihydroxy-3-(hydroxymethyl)-2 -Cyclohexenyl-1-amino]-a-D-glucopyranosyl-(1→4)—O—a—D-glucopyranosyl—(1→4)-D-glucopyranosyl, which is composed of free The metabolites produced by Actinoplanes species. are mainly used in the treatment of type II diabetes. [0003] In the industry, acarbose is prepared by regulating the microbial metabolism of Actinoplanes species, that is, by fermentation. The feeding method generally adopted in the microbial fermentation method is periodic feeding, feeding once every other day, and a fermentation cycle takes about 8 days, that is, feeding 3-4 times in a fermentation cycle. This feeding method has great disadvantages. Because it is a one-time supplement of carb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12P19/26C12R1/045
Inventor 周鲁瑾章飞龙叶敏
Owner HUADONG MEDICINE
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