Device for measuring fluorescence life time
A fluorescence lifetime and measurement method technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of large volume, complex measurement system composition, high system cost, convenient portability, shortened fitting time, and improved signal-to-noise. the effect of
Inactive Publication Date: 2009-07-29
HARBIN ENG UNIV
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The composition of the entire measurement system is very complex (in addition to photoelectric conversion devices and amplifier circuits, there are at least computers and high-speed, high-precision data acquisition cards, etc.), the system cost is high, and the volume is large, which is not conducive to the miniaturization and practical application of this technology.
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[0037] Below in conjunction with accompanying drawing, the measuring device of fluorescence lifetime of the present invention is described in further detail:
[0038] figure 1It is a structural schematic diagram of the present invention based on the fast Fourier transform of the digital processing chip. First, the photodetector is used to receive the fluorescent signal, and the collected fluorescent attenuation signal is amplified and converted to analog-to-digital A / D, and sent to the complex programmable logic device CPLD, and the collected signal is sent to the digital processing chip DSP chip, and the Complete the fast Fourier transform in the processing chip, select the spectral item to obtain the spectrum, obtain the argument angle of the corresponding spectral item, calculate the argument angle or the tangent value of the argument angle of the spectral item, and further use the formula to calculate the fluorescence lifetime corresponding to the decay curve Value, outp...
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The invention provides a fluorescent lifetime measurement device based on a digital processing chip, which uses a photodetector to receive a fluorescent signal, and sends it to a digital processing chip after signal amplification and analog-to-digital conversion, and completes fast Fourier transform in the digital processing chip to obtain The argument of the corresponding spectrum item, and then use the formula to complete the calculation of the fluorescence lifetime in the processing chip, output or display the measured fluorescence lifetime, or give the measured other parameters (such as temperature, etc.) as a reference signal, and finally pass The measured fluorescence lifetime and other parameters are sent to the computer with an asynchronous receiver and transmitter. The invention has the characteristics of high speed, high precision, no influence of local noise, low cost, miniaturization and the like for realizing fluorescence lifetime measurement, and is a new generation of fluorescence lifetime measuring device.
Description
(1) Technical field [0001] The invention relates to a measurement method, in particular to a measurement method for measuring fluorescence lifetime based on fast Fourier transform of a digital processing chip. (2) Background technology [0002] Rare earth element doped substances will emit fluorescence when excited by the pump light, and this fluorescence will decay exponentially after the pump light stops, and the time for the intensity of the fluorescence signal to decay to the original 1 / e is called is the fluorescence lifetime. [0003] The fluorescence lifetime is related to many properties of the substance that emits fluorescence, such as the temperature, humidity, pH value, etc. of the substance. Therefore, measuring the fluorescence lifetime can indirectly measure various physical and chemical parameters. Some people at home and abroad have tried to develop various sensors based on fluorescence lifetime measurement. [0004] For example, fluorescent temperature sen...
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IPC IPC(8): G01N21/64
Inventor 孙伟民李海森姜海丽陈宝伟张建中张杨姜富强张志林
Owner HARBIN ENG UNIV