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Reagent kit for detecting early stage cancer

An early detection and kit technology, applied in the fields of medicine and pharmacy, can solve the problems of inconvenient promotion, high detection cost, and inconvenient use, and achieve the effects of easy promotion, low cost, and easy acquisition.

Inactive Publication Date: 2009-10-21
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Determination of the light absorption of NADH at 340nm requires a UV photometric analyzer or a special analyzer, and requires specialized technical personnel to operate, which is not very convenient to use, the detection cost is high, and it is not easy to promote

Method used

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  • Reagent kit for detecting early stage cancer

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Experimental program
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Embodiment Construction

[0015] 1. Separation and extraction of L-fucose dehydrogenase

[0016] Extract L-fucose dehydrogenase from fresh pork liver: Homogenize 250 grams of fresh pork liver with a tissue masher, then add 1L 0.25mol / L sucrose, centrifuge at 25000g for 20min, discard the precipitate and take the supernatant, add 450ml to saturate Ammonium sulfate, centrifuge at 25000g for 20min after standing for 15min, discard the precipitate and take the supernatant, add 400ml saturated ammonium sulfate, centrifuge at 25000g for 20min after standing for 15min, discard the supernatant to remove the precipitate, dialyze with 0.005M phosphate buffer saline (PBS), and take the dialysate Diethylaminoethyl (DEAE) cellulose column (DE 52 ), eluted with a gradient of 0.005mol / L phosphate buffered saline (PBS) containing 0.05-0.15mol / L sodium chloride (NaCl), and collected the eluted fraction of 0.1-0.15mol / L sodium chloride (NaCl) Concentrate, measure L-fucose dehydrogenase, and store in a -70°C refrigerato...

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Abstract

The invention discloses a kit and method for detecting early cancer. The kit includes A-liquid, B-liquid and C-liquid. The -70°C refrigerator is for standby; the second is to use the solution in the kit; then take the patient's urine, add A-liquid, B-liquid, and C-liquid respectively, keep warm, and observe the reaction. The test results are very intuitive and visible to the naked eye. The effect is high, and the positive rate of digestive tract cancer can reach 85-90%. The method of the invention is easy to operate, and the operation is convenient for ordinary medical personnel.

Description

technical field [0001] The present invention relates to the fields of medicine and pharmacy. More specifically, it relates to a kit for detecting early cancer, which is suitable for the detection of digestive tract cancer, and also relates to a detection method. Background technique [0002] According to statistics, there are about 1.6 million new cancer patients in my country every year, and about 1.3 million people die from cancer every year. Among the many causes of death among residents in large and medium-sized cities in my country, cancer is the first cause of death, and among various causes of death in rural areas, cancer is the second cause of death. For middle-aged and elderly people, cancer is a frequently-occurring disease and a common disease. According to the current medical level, more than 80%-90% of early cancer patients can be cured; however, there are fewer advanced cancer patients who can survive for more than 5 years after treatment. After the treatmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N33/50G01N1/34
Inventor 吴建国邬开朗刘芳朱应祝成亮
Owner WUHAN UNIV