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Method for preparing peroxidase of radish

A technology of peroxidase and radish, applied in the biological field, can solve the problems of high cost, restricted development and utilization, and complicated extraction process.

Inactive Publication Date: 2007-07-25
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the suitable material for extracting peroxidase is not easy to screen, the extraction process is complicated, and the reasons such as high cost restrict its development and utilization, especially the method for preparing peroxidase with fresh radish as raw material, although there are reports at home and abroad
However, there is no report on the preparation of radish peroxidase by direct salting-out method

Method used

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Embodiment Construction

[0005] The specific embodiment of the present invention is as follows: take 1 ton of fresh radish, crush it into a paste, place it in a high-speed refrigerated centrifuge to separate the radish juice, and then extract the radish residue with buffer solution, the volume increases by 3-5 times, but the obtained The peroxidase is only 1 / 10 of the original juice. When the obtained radish juice is added to ammonium sulfate powder step by step to make the saturation reach 30-50%, let it stand for 10-18 hours. After discarding the sediment, take Supernatant, continue to add ammonium sulfate powder to the supernatant to make the saturation reach 75-90%, let it stand for 10-18 hours, then discard the supernatant, dilute the precipitate with water, filter, and filter the filtered filtrate Use 20×17cm, 100kD PES ultrafiltration membrane for ultrafiltration and desalination, the permeate that passes through the membrane can be further recycled, and the concentrated solution that does not p...

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Abstract

This invention discloses a method for preparing radish peroxydase. The method comprises: (1) pulverizing fresh radish at 0-15 deg.C, and separating the juice; (2) adding ammonium sulfate 30-50% into the juice, standing for 10-18 h, removing the precipitate, collecting the supernatant, adding ammonium sulfate 75-90%, standing for 10-18 h, decanting the supernatant, collecting the precipitate, diluting with water, and filtering; (3) filtering with an ultrafiltration membrane, recovering the filtrate, and collecting the concentrated liquid; (4) freeze-drying the concentrated liquid to obtain crude radish peroxydase, and performing column chromatography to obtain purified radish peroxydase. The method has such advantages as low peroxydase activity loss, low energy consumption, and easy operation. The radish peroxydase is safe and effective when used in animals and plants.

Description

Technical field: [0001] The invention relates to biotechnology, in particular to a method for extracting peroxidase using fresh radish as raw material with salting out, ultrafiltration and dialysis devices. Background technique: [0002] Peroxidase is widely present in organisms, and has the functions of removing peroxide, protecting cells from being damaged by peroxide, and removing skin pigmentation. It has broad application prospects in the fields of food and cosmetic additives and biochemical reagents Greater economic benefits. However, because the suitable material for extracting peroxidase is not easy to screen, the extraction process is complicated, and the reasons such as high cost restrict its development and utilization, especially the method for preparing peroxidase with fresh radish as raw material, although there are reports at home and abroad . However, there is no report on the preparation of radish peroxidase by direct salting-out method. Invention conten...

Claims

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Application Information

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IPC IPC(8): C12N9/08
Inventor 王林嵩王琳马剑敏徐存拴王丽车淑红王歌
Owner HENAN NORMAL UNIV
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