75results about How to "Little loss of enzyme activity" patented technology

The invention provides an immobilized transaminase obtained via immobilizing recombined transaminase on sodium alginate. Firm bonding of the immobilized transaminase is realized; enzyme activity loss is less; separation is simple; recycling can be realized for a plurality of times; asymmetric conversion process cost is low; reaction conditions are mild; the immobilized transaminase is friendly to the environment; operation is simple; industrial enlarged production is convenient to realize; and industrial application prospect is promising.

The invention relates to the method used to hydrolyze DL-fat acid mint ester and produce L-menthol by full cell biological method stereo-selectivity. It supplies the high stereo-selectivity strain, produces full cell, uses the DL-fat acid mint ester as substrate, hydrolyzes and produces L-menthol by full cell biological method stereo-selectivity. The by-product D-fat acid mint ester can be hydrolyzed to high purity D-menthol of which optical purity is 80%e.e-100%e.e; yield is 30%-60% while producing L-menthol of which optical purity is 90%e.e-100%e.e; conversion rate is 40%-50%. Compared with the international L-menthol preparation method, the adopted full cell catalysis can omit fussy enzyme purification, reduce enzyme activity damage, increase enzyme action substrate range and operation stability, and reduce L-menthol production cost.

The invention discloses a separation and purification method and an immobilization method of papain, and a product thereof. The separation and purification method comprises the following steps: 1, obtaining an initial crude enzyme solution through dissolving a raw material containing papain, removing insoluble substances, and adjusting the protein concentration to 10-20mg / ml; 2, mixing the initial crude enzyme solution with polyethylene glycol, an inorganic salt and water, dissolving, and carrying out phase separation after all component are uniformly mixed, wherein the content of the initial crude enzyme solution is 30-40%, the content of polyethylene glycol is 10-30%, the content of the inorganic salt is 10-25%, and the pH value is 5.5 to 6.5; and 3, taking a polyethylene glycol phase obtained after the phase separation, and recovering the papain through an ion exchange method. The immobilization method is that papain is immobilized in the polyethylene glycol phase which is enriched in papain. The separation and purification method allows good biological compatibility, simple operation steps, and mild operation conditions to be realized, processes to be easily amplified, less enzyme activity loss of products and no organic solvent residual to be achieved, and immobilization steps to be substantially simplified.

The invention discloses a feed compound enzyme capable of improving animal appetite and a preparation method of the feed compound enzyme, and belongs to the technical field of preparation of an enzyme preparation. The feed compound enzyme is scientifically compounded from fragrant malt enzyme, feeding complex enzyme, acidic xylanase, cellulose, beta-glucanase, amylase, phytase, Chinese herb extracts, a protective agent and an activating agent, wherein the feed complex enzyme contains protease, mannose, alpha-galactase and pectinase, and is strong in heat stability and pH stability. By adopting the prepared feed compound enzyme, safe digestive enzyme is provided for beasts and birds, the digestion burden is effectively relieved, the utilization rate and the growth rate of the raw materials are improved, the environment is protected, an enjoyable natural malt fragrance is brought about for the feed, the livestock and poultry appetites are increased, meanwhile, proper activating agent can fully exert the efficacy of an enzyme preparation under the equal conditions, the adding amount of the enzyme preparation is saved, the guarantee period of the complex enzyme preparation is prolonged by the Chinese herbal medicine extract, the immunity of beasts and birds is improved, and the multi-purpose effect of the enzyme is achieved.

The invention discloses aspergillus niger capable of highly yielding a compound enzyme for a feed and an application thereof, and belongs to the technical field of microbial fermentation. The strain of the aspergillus niger specifically is aspergillus niger DM-18 which is collected in the China Center for Type Culture Collection with the collection number of CCTCC M2013541. A crude enzyme which is obtained through culture medium optimization and fermentation for 72-96 hours contains a plurality of kinds of enzymes, wherein the activity of protease is 6500U / ml, the activity of mannase is 1500U / ml, the activity of alpha-galactase is 1200U / ml and the activity of pectase is 1000U / ml. The optimum pH value for the activity of each enzyme in the compound enzyme ranges from 2 to 7, and is suitable for the digestion and absorption environment of the gastrointestinal tract of the animal; the compound enzyme for feed can be widely applied to the feeds for beasts and birds, is capable of reducing the antinutritional factors and increasing the digestibility, and has excellent economic benefits; simultaneously, the compound enzyme is also advantageous for protecting the economic environment and promoting healthy development of the animal husbandry.

The invention relates to a method for fossilizing pectinase by using nanometer silicon material. The method is characterized in that novel nanometer silicon material is prepared by using a layer-by-layer assembly technique, activation, modification and coating are performed on the material, and efficient immobilization to pectinase is achieved through glutaraldehyde crosslink. The carrier for immobilization, prepared in the invention has the advantage of high protein carrying capacity, and the obtained immobilized pectinase enzyme has the characteristics of small enzyme activity loss and low probability of enzyme dropping, and can be used repeatedly.

The invention provides a preparation method of an immobilized lipase with a purified cotton towel as a carrier. The method is an immobilization technique comprising the following steps of: adsorbing a polyethyleneimine aqueous solution with the purified cotton towel, adding a lipase solution after adsorption, vibrating with a constant-temperature shaking table, thus attaching the lipase to the purified cotton towel containing polyethyleneimine, removing the supernatant, and then cross-linking the purified cotton towel with the lipase and polyethyleneimine adsorbed by using a cross-linking agent. According to the invention, the towel carrier used by the preparation method has multiple gaps and long towel fibers, thus having a larger surface area, favoring the performing of immobilized-enzyme-catalyzed reaction, having very good mechanical strength, and the method can more easily separate the enzyme from the reaction system, compared with the granular carrier; the used cross-linking agent is tris(hydroxymethyl)phosphine which has a better cross-linking effect than glutaraldehyde; the used immobilization method has the advantages of mild conditions and multi-layer immobilization; and the obtained immobilized lipase has good temperature stability and high reaction activity, can shorten the reaction time and can be repeatedly used.

The invention discloses a method for producing 1,5-pentanediamine through immobilized lysine decarboxylase. The method comprises the following steps of connecting a chitin binding domain (ChBD) gene to the N end of a lysine decarboxylase gene (CadA) to construct a fusion protein; fermenting the fusion protein to obtain a crude lysine decarboxylase enzyme solution; adding chitin into the crude enzyme solution to obtain immobilized lysine decarboxylase; finally, enabling the obtained immobilized lysine decarboxylase to participate in a decarboxylation reaction of L-lysine to prepare 1,5-1,5-pentanediamine. According to the method, the specific affinity effect of the substrate chitin and the fusion protein is skillfully utilized to adsorb the lysine decarboxylase, the cost of the immobilizedcarrier chitin is low, the immobilization efficiency is high, the activity stability of enzymes is high, and meanwhile, the procedure of protein purification can be omitted through specific affinity adsorption. The provided method achieves repeated production of 1,5-1,5-pentanediamine and can reduce the production cost, simplify the separation procedure of downstream products and enzymes and achieve remarkable economic benefits.

The invention discloses a method for preparing biodiesel by catalyzing vector-free immobilized lipase in an ionic liquid system. The method comprises the following two steps: (1) preparing vector-free immobilized lipase; and (2) preparing the biodiesel by catalyzing the prepared vector-free immobilized lipase in the ionic liquid system. The prepared vector-free immobilized lipase is high in activity and free of high-cost vectors; an ionic liquid medium is used as an immobilized enzyme for preparing the biodiesel; the stability and the stereoselectivity of substrates of the enzyme can be obviously improved; the problem of the enzyme poisoning caused by methyl alcohol and glycerol can also be avoided; the efficient catalysis of the enzyme is ensured; the yield of the biodiesel is greatly increased; the immobilized enzyme and the ionic liquid used in the method can be recycled and reused; the waste of resources is avoided; the production cost is reduced; the method has a good industrial application prospect.

The physical papain extracting process includes the following steps: forming papaya slurry, freezing, pre-treating, coarse filtering, fine filtering, twice ultrafiltering, and drying to obtain product. Compared with available chemical extraction process, the present invention has the advantages low cost, simple technological process, short drying time, low power consumption, high enzyme activity of the product, high heat stability of the product, etc.

The invention discloses a technological method for removing glucose in an isomaltooligosaccharide product by using multiple immobilized enzymes. The technological method is used for realizing multiple enzyme separated immobilization by taking calcium carbonate as a template and combining bionic silicification and bionic bonding thoughts on the basis of a layer-by-layer self-assembly technology so as to remove the glucose in the isomaltooligosaccharide product. The technological method is simple in preparation process, mild in condition and little in enzyme activity loss; the prepared immobilized enzymes are used for removing the glucose in the isomaltooligosaccharide product at a high removal rate up to over 80%, and are relatively high in repeated utilization ratio; a carrier is cheap and easy to obtain.

The invention discloses a high-activity composite enzyme for feed, belonging to the technical field of preparation of an enzyme preparation for feed. An Aspergillus niger DM-18 strain for a high-yield composite enzyme for feed, which is used as an initial strain, is subjected to liquid submerged mixed fermentation in an optimized culture medium under specific fermentation conditions by an improve fermentation technique to obtain the composite enzyme for feed, which has the advantages of complete enzymatic system, high enzyme activity, high temperature resistance and wide pH value resistance range. The crude enzyme fermentation liquid of the high-activity composite enzyme for feed contains multiple enzymes, wherein the proteinase activity is 6500-6700 U / ml, the mannase activity is 1500-1700 U / ml, the alpha-galactase activity is 1200-1300 U / ml, and the pectinase activity is 1000-1200 U / ml. When the crude enzyme fermentation liquid is subjected to the heat stability test and pH stability test, the test result indicates that all the enzymes have higher enzyme activity (up to higher than 80%) at 30-75 DEG C under the pH value of 2-7.

The invention discloses a bromelain stabilizer and a preparation method and application thereof, and relates to the technical field of biology. The bromelain stabilizer is prepared from the followingcomponents in parts by weight: 0.015 to 0.13 part of reductant, 0.002 to 0.07 part of metal ion chelating agent, 0.01 to 0.09 part of organic carboxylate, 0.05 to 0.15 part of preservative, and 0.001to 0.02 part of vitamin. The bromelain stabilizer has the advantages that the effect of protecting enzyme activity is obvious, the loss of enzyme activity is reduced, the half-life period of enzyme isshortened, the usage amount is small, the cost is low, and the like. The preparation method has the advantages that the operation is simple, and the preparation method is suitable for industrializedproduction; the bromelain stabilizer can be directly added inr the process of storage of enzyme solid powder or production of liquid enzyme, and the application range is broad.

The invention provides a method for preparing catalase by using bovine livers, wherein fresh bovine livers are used as raw materials and subjected to chopping and homogenization, and a catalase product with high enzyme activity is obtained by means of processes such as ultrasonic extraction, ion exchange chromatography, gel filtration chromatography and freeze drying. The method of the invention solves the problems of complex production process, heavy pollution, high production cost and low product yield of the prior art in preparing the product. The catalase prepared by the method of the invention has an enzyme activity of 6000 U / mg and a colony count of less than or equal to 1000 cells / ml, and is suitable to be widely used in the fields of food, paper making and textile industry.

The invention discloses a catfish feed compound enzyme and a preparation method thereof. The preparation method of the catfish feed compound enzyme is characterized by taking nutritional requirement of the catfish and the water environment as the basis, optimizing the raw material formula, combining digestion characteristics of the catfish and scientifically compounding a wine making yeast culture material with functional characteristics such as high nutrition performance, high biological activity, high oxidation resistance and the like, a calcium fruit extract capable of effectively supplementing calcium, iron and soluble fiber, providing powerful embedding effect and prolonging the quality guarantee period of the feed compound enzyme, wormwood seed powder capable of obviously improving anti-freezing effect and stability of the feed compound enzyme, improving water resistance and glossiness of the catfish feed and reducing powder content in the feed, a palatable agent capable of greatly improving palatability and digestion rate of the feed, and wheat malt flour capable of providing rich nutrient materials, biological active materials and plant enzyme sources to finally obtain the catfish feed compound enzyme capable of obviously enhancing digestion rate and parturition performance of the catfish, reducing the feed coefficient, preventing water pollution and maintaining high water quality.

The invention discloses a lysozyme with covalent modification through medium and long fat chain acyl-free sophorolipid derivatives (DSL-C12) and a modifying method and application of the lysozyme. The modifying method comprises the following steps that firstly, active esters of sophorolipid derivatives are dissolved in DMSO, then a NaHCO3 aqueous solution of lysozyme is added, a condensation reaction is carried out under the stirring condition, and a reaction mixture is obtained after the reaction is finished, wherein the sophorolipid derivatives are carboxylic acid type medium and long fat chain sophorolipid; secondly, deionized water and a phosphate buffer solution are adopted in sequence to carry out dialysis on the mixture obtained in the first step, obtained dialysate is subjected to centrifugation separation, and the modified lysozyme is obtained. The modified lysozyme can effectively suppress the activity of gram-positive bacteria and can also remarkably suppress the activity of gram-negative bacteria, so that the antibacterial spectrum of natural lysozyme is expanded, and the lysozyme can serve as a multifunctional food preservative.

This invention discloses a method for preparing radish peroxydase. The method comprises: (1) pulverizing fresh radish at 0-15 deg.C, and separating the juice; (2) adding ammonium sulfate 30-50% into the juice, standing for 10-18 h, removing the precipitate, collecting the supernatant, adding ammonium sulfate 75-90%, standing for 10-18 h, decanting the supernatant, collecting the precipitate, diluting with water, and filtering; (3) filtering with an ultrafiltration membrane, recovering the filtrate, and collecting the concentrated liquid; (4) freeze-drying the concentrated liquid to obtain crude radish peroxydase, and performing column chromatography to obtain purified radish peroxydase. The method has such advantages as low peroxydase activity loss, low energy consumption, and easy operation. The radish peroxydase is safe and effective when used in animals and plants.

The invention discloses a method for extracting and obtaining a bromelain from pineapple peel residues, which comprises the following steps of: cleaning pineapple peel residues, putting the pineapplepeel residues into a crushing and filtering device for multi-stage crushing, adding 0.05-0.1 % of ascorbic acid and 0.5-1 Kg of distilled water, and finally obtaining filtrate through extrusion and filtration; adding 0.03-0.05 % of calcium propionate into the filtrate for corrosion prevention, stirring and centrifuging the filtrate at a high speed for 10-15 minutes at a stirring speed of 4000-5000r / min, taking supernatant, adding pre-dissolved tannin solution into the supernatant, stirring while leading the final concentration of the tannin to be 0.1-0.2 %, and allowing the solution to standafter adding the tannin. The enzyme activity of the bromelain prepared from the pineapple peel residues is good, the purity is high, and the utilization rate of pineapple peel residues is improved.

The invention provides a method for extracting purified Tth DNA polymerases. The method comprises the following steps: taking engineering bacteria of Tth DNA polymerases for activation, and adding IPTG for inducible expression; adding DTT and tween-20 after the engineering bacteria are treated with lysozyme at room temperature, treating for a period of time on ice and then centrifuging to obtain crude protein; slowing adding silicon dioxide particles in the crude protein, stirring in a magnetic stirring apparatus, centrifuging to remove precipitates; allowing supernatant to flow through an Ni<2+>-chelated Ni-NTA column after the supernatant is supplemented with NaCl, then washing sequentially with washing buffer solutions which have 5 times of bed volume, and at last, eluting the target protein with an eluent, and receiving the eluent containing the target protein; desalting to obtain the purified Tth DNA polymerases; adding glycerol in the obtained purified Tth DNA polymerases, so that the volume fraction is 50%, and saving at -20 DEG C. The method is simple, small in enzyme activity loss, and high in purity and recovery capacity of the recovered protein.

The invention discloses a green preparation method of high-purity galactooligosaccharide. The green preparation method comprises the following steps of fermenting preparation of yeast whole cell containing beta-galactosidase endoenzyme, permeabilizing treatment of yeast whole cell by an ethanol solution, preparation of galactooligosaccharide by permeabilized yeast cell catalyzing lactose (purpose of yeast I), and obtaining of high-purity galactooligosaccharide (purpose of yeast II) by using yeast whole cell reaction to purify a crude product of galactooligosaccharide. The green preparation method has the advantages that the permeable yeast cell and the yeast whole cell can be repeatedly utilized, so that the effect of one yeast, two purposes is realized in the green cycling preparation of the high-purity galactooligosaccharide; the purity of the obtained high-purity galactooligosaccharide can reach 99% to the highest degree, the technology is simple, no harmful matter is added, the required equipment cost is low, and the high-purity galactooligosaccharide is suitable for being widely and industrially popularized.

The invention discloses a lysozyme with covalent modification through acyl-free sophorolipid derivatives and a modifying method and application of the lysozyme. The modifying method comprises the following steps that firstly, active esters of acyl-free sophorolipid derivatives are dissolved in DMSO, then a NaHCO3 aqueous solution of lysozyme is added, a condensation reaction is carried out under the stirring condition, and a mixture with covalent modification is obtained after the reaction is finished, wherein the sophorolipid derivatives are carboxylic acid type acyl-free sophorolipid; secondly, deionized water and a phosphate buffer solution are adopted in sequence to carry out dialysis on the butter solution system obtained in the first step, obtained dialysate is subjected to centrifugation separation, and the lysozyme with covalent modification is obtained. The modified lysozyme can effectively suppress the activity of gram-positive bacteria, can also remarkably suppress the activity of gram-negative bacteria, and can serve as a multifunctional food preservative.

The invention discloses a nattokinase drying protective agent. The nattokinase drying protective agent comprises trehalose and maltodextrin, wherein the mass ratio of the trehalose to the maltodextrinis 2:(1-4). A preparation method comprises the steps: respectively weighing the trehalose and the maltodextrin according to the mass ratio being 2:(1-4) of the trehalose to the maltodextrin, adding the trehalose and the maltodextrin to distilled water, and stirring to obtain the nattokinase drying protective agent. A nattokinase drying method comprises the following steps: mixing a nattokinase crude enzyme with the nattokinase drying protective agent according to the volume ratio of 1: (2-5), homogenizing, carrying out spray drying, and collecting nattokinase enzyme powder, wherein the average value of the enzyme activity of produced nattokinase is (210200+ / -19126)FU / g. The nattokinase drying method has the advantages that (1) bean dregs are used as a matrix to produce the nattokinase, the added value of the bean dregs is increased, and the enzyme activity of the nattokinase is comparable to the enzyme activity of the nattokinase produced by using soybeans as the matrix; and (2) the loss of the enzyme activity in the drying process is reduced, and the stability of the nattokinase is improved.

The invention discloses a method for producing laccase through solid-state fermentation of pseudo-ginseng residues. The method comprises the following steps: (1) drying, crushing and screening the pseudo-ginseng residues; (2) uniformly mixing the pretreated pseudo-ginseng residues, a proper amount of nitrogen source, water, copper sulfate, magnesium sulfate, monopotassium phosphate, tween-80 and veratryl alcohol according to a certain proportion; (3) performing steam sterilization treatment on the uniformly mixed materials; (4) inoculating at least one ganoderma lucidum on the sterilized material for solid state fermentation; and (5) drying the fermentation culture to obtain the ganoderma lucidum laccase. By adopting the method disclosed by the invention, the pseudo-ginseng residues can be converted into laccase. According to the method, the pseudo-ginseng residues are subjected to resource utilization, waste is turned into wealth, pollution caused by the pseudo-ginseng residues to the environment is reduced, and low-cost laccase can be provided for the environmental protection industry.

The invention discloses a method for extracting endoenzyme generated by fungal mycelium liquid submerged fermentation. The method comprises the following steps: separating a fermentation liquor to obtain mycelia, diluting the mycelia with water, and performing beating processing and ultrasonication processing on the diluted mycelia to obtain a crude enzyme. Through combination of the beating processing and the ultrasonication processing, the crude enzyme of endoenzyme generated by fungal mycelium liquid submerged fermentation is obtained, so that the loss of enzyme activity is less. The method provided by the invention is simple in operation, convenient, feasible and suitable for the extraction of enzymes generated by liquid submerged fermentation of various fungal mycelia such as ganoderma, champignon and Grifola frondosa.

The invention discloses a fermentation liquor treatment method for increasing the stability of pullulanase, and belongs to the technical field of enzyme engineering and fermentation engineering. The fermentation liquor treatment method is characterized in that pullulanase fermentation liquor sourcing from recombinant bacteria-bacillus amyloliquefaciens BF7658 / pUC980-BdP is treated by using 10*fermentation liquor treatment solution, and the enzyme activity stability of the pullulanase in the treated fermentation liquor is obvious higher than that of untreated fermentation liquor. According to the fermentation liquor treatment method for increasing the stability of the pullulanase, disclosed by the invention, in a post-extraction stage of a technology for preparing the pullulanase by taking the recombinant bacteria-bacillus amyloliquefaciens BF7658 / pUC980-BdP as a strain for fermentation, the loss of enzyme activity can be reduced.

The invention relates to a method for extracting and purifying superoxide dismutase (SOD), and in particular relates to a method for extracting and purifying SOD from saccharomyces cerevisiae. The method comprises the following operation steps: collecting bacteria, homogenizing bacteria, preparing a crude enzyme fluid, carrying out ultrafiltration, implementing chromatography and desalting. The method disclosed by the invention can be used for effectively purifying non-modified SOD protein and keeping the original activity of the SOD; and through a pyrogallol oxidation method, the specific activity of the purified high-purity SOD can reach up to 15000U / g and the entire process is relatively high in yield; and the method is simple to operate and capable of achieving industrial mass production.

The invention relates to a production method of a pesticide residue fast detection card based on horse serum cholinesterase. The method comprises precipitating and setting cholinesterase in horse serum through a salting-out method, carrying out purification through centrifugation, redissolution and dialysis to obtain cholinesterase satisfying the requirements, carrying out dilution according to the activity of the cholinesterase, adjusting pH and a salt ion concentration, adding a stabilizer into the solution to obtain a pesticide residue fast detection card enzyme work solution, pressing twocircular filter papers on a support substrate at a high temperature, adding a cholinesterase working solution and a substrate working solution on the two filter papers drop by drop, carrying out airing and carrying out plastic packaging to obtain the pesticide residue fast detection card. The pesticide residue fast detection card has good sensitivity, stability, repeatability, reproducibility, simplicity, rapidness, intuitiveness and low cost and is suitable for rapid screening of pesticide residues on fruits and vegetables.

The invention discloses a resin-enzyme composite catalyst and a preparation method thereof, belonging to the field of composite materials. The method comprises the following steps: dissolving a target enzyme in a phosphate buffer with pH value being neutral so as to obtain a solution A with concentration of 0.2mg / mL-10mg / mL; selecting a suitable large-hole type ion exchange resin as a carrier according to isoelectric points of an enzyme molecule; mixing and stirring the solution A and the resin carrier at room temperature; and taking out the resin, washing the surface of the resin by utilizing the phosphate buffer and drying the resin to obtain the resin-enzyme composite catalyst. The preparation method provided by the invention has the advantages of simplicity in operation, easiness in control and less loss of enzymatic activity; the fixed-load enzyme composite catalyst prepared by the method provided by the invention ensures that the stability of ordinary enzyme is greatly improved on the basis of maintaining the original catalytic activity of the enzyme molecules, can be applied to a water phase system and has the characteristic of good leaching-resisting property within a wide pH range and under high ionic strength.

The invention discloses a method for producing laccase through mixed bacteria solid-state fermentation of pseudo-ginseng residues. The method comprises the following steps: (1) drying, crushing and screening the pseudo-ginseng residues; (2) uniformly mixing the pretreated pseudo-ginseng residues, a proper amount of nitrogen source, water, copper sulfate, magnesium sulfate, monopotassium phosphate, tween-80 and veratryl alcohol according to a certain proportion; (3) performing steam sterilization treatment on the uniformly mixed materials; (4) inoculating at least one kind of ganoderma lucidum and at least one kind of saccharomycetes on the sterilized material for mixed bacteria solid-state fermentation; and (5) drying the fermentation culture to obtain the ganoderma lucidum laccase. By adopting the method disclosed by the invention, the pseudo-ginseng residues can be converted into laccase. According to the method, the pseudo-ginseng residues are subjected to resource utilization, waste is turned into wealth, pollution caused by the pseudo-ginseng residues to the environment is reduced, and low-cost laccase can be provided for the environmental protection industry.

The invention is applicable to the technical field of biochemistry, in particular to a calibrator for a biochemical calibrator and the biochemical calibrator. Each liter of the matrix for the biochemical calibrator comprises the following components of 10 to 100 mmol of a pH regulator, 2-50 g of a saccharide protective agent, 2-100 g of a polymer protective agent, 5-50 g of a protein protective agent, 0.5 to 5 grams of surfactant, 5 to 20 millimoles of amino acid, 0.02 to 50 millimoles of antioxidant, 0.05 to 2 millimoles of protease inhibitor, 5 to 30 grams of alcohol organic solvent, 0.2 to0.5 gram of preservative, 8.5 to 9.5 grams of sodium chloride and 0.1 to 5 millimoles of magnesium salt and / or zinc salt. According to the calibrator for the biochemical calibrator, when the analysiscomponents are added into the matrix for the biochemical calibrator, the components in the matrix for the biochemical calibrator can play a better role in protecting the analysis components, particularly, the structural variation caused by water loss of the analysis components in the freeze-drying process can be avoided, and the effect of the analysis components is effectively reserved.