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Technological method for removing glucose in isomaltooligosaccharide product by using multiple immobilized enzymes

A technology of isomaltose oligosaccharide and a process method, applied in the application field of immobilized enzymes, can solve the problems of influence of catalytic performance, difficulty in forming a substrate product chain, poor independent regulation, etc., and achieves small loss of enzyme activity, mild conditions, and repeatability. High usage effect

Active Publication Date: 2014-01-22
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to address the current co-immobilized multi-enzyme system (1) the independent regulation of various enzymes is poor; (2) the immobilization position of various enzymes is random, and it is difficult to achieve the purpose of forming a substrate product chain ; (3) The mutual contact of multiple enzymes may lead to the disadvantage that their respective catalytic performances are affected. This provides a simple and low-cost method for immobilizing multiple enzymes in a compartmentalized manner, and this immobilized multiple enzymes are used to remove oligomers. Glucose in isomaltose products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] ①Take 0.33mol / L of CaCl 2 2H 2 O solution 10ml, add 300mgPSS and 800UGOD in it, then add 10ml of 0.33mol / L Na under the magnetic stirrer (600r / min) 2 CO 3 Solution, make it continue to stir for 20s, let it stand for 20min, shake well, centrifuge, remove the supernatant, wash with water to obtain calcium carbonate particles; ②add 15ml of 2mg / ml PSS solution to all the above calcium carbonate particles, and shake for 15min , centrifuge, remove the supernatant, and wash with water to obtain PSS-wrapped calcium carbonate particles; then add 15ml of 2mg / ml polydimethyldiallyl ammonium chloride (PDADMAC) solution to the PSS-wrapped calcium carbonate particles, shake 15min, centrifugal, remove supernatant, wash with water, obtain the calcium carbonate particle of an organic double layer that is wrapped with PSS and PDADMAC, be called for short (PSS / PDADMAC) 1 -CaCO 3 ;backward (PSS / PDADMAC) 1 -CaCO 3 Add 15ml of 2mg / ml PSS solution, shake for 15min, centrifuge, remove th...

example 2

[0035] ①Take 0.33mol / L of CaCl 2 2H 2 O solution 10ml, add 300mgPSS and 800UGOD in it, then add 10ml of 0.33mol / L Na under the magnetic stirrer (600r / min) 2 CO 3 Solution, make it continue to stir for 20s, let it stand for 20min, shake well, centrifuge, remove the supernatant, wash with water to obtain calcium carbonate particles; ②add 15ml of 2mg / ml PSS solution to all the above calcium carbonate particles, and shake for 15min , centrifuge, remove the supernatant, and wash with water to obtain PSS-wrapped calcium carbonate particles; then add 15ml of 2mg / ml polydimethyldiallyl ammonium chloride (PDADMAC) solution to the PSS-wrapped calcium carbonate particles, shake 15min, centrifugal, remove supernatant, wash with water, obtain the calcium carbonate particle of an organic double layer that is wrapped with PSS and PDADMAC, be called for short (PSS / PDADMAC) 1 -CaCO 3 ;backward (PSS / PDADMAC) 1 -CaCO 3 Add 15ml of 2mg / ml PSS solution, shake for 15min, centrifuge, remove th...

example 3

[0039] ①Take 0.33mol / L of CaCl 2 2H 2 O solution 10ml, add 300mgPSS and 800UGOD in it, then add 10ml of 0.33mol / L Na under the magnetic stirrer (600r / min) 2 CO 3Solution, make it continue to stir for 20s, let it stand for 20min, shake well, centrifuge, remove the supernatant, wash with water to obtain calcium carbonate particles; ②add 15ml of 2mg / ml PSS solution to all the above calcium carbonate particles, and shake for 15min , centrifuge, remove the supernatant, and wash with water to obtain PSS-wrapped calcium carbonate particles; then add 15ml of 2mg / ml polydimethyldiallyl ammonium chloride (PDADMAC) solution to the PSS-wrapped calcium carbonate particles, shake 15min, centrifugal, remove supernatant, wash with water, obtain the calcium carbonate particle of an organic double layer that is wrapped with PSS and PDADMAC, be called for short (PSS / PDADMAC) 1 -CaCO 3 ;backward (PSS / PDADMAC) 1 -CaCO 3 Add 15ml of 2mg / ml PSS solution, shake for 15min, centrifuge, remove the...

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Abstract

The invention discloses a technological method for removing glucose in an isomaltooligosaccharide product by using multiple immobilized enzymes. The technological method is used for realizing multiple enzyme separated immobilization by taking calcium carbonate as a template and combining bionic silicification and bionic bonding thoughts on the basis of a layer-by-layer self-assembly technology so as to remove the glucose in the isomaltooligosaccharide product. The technological method is simple in preparation process, mild in condition and little in enzyme activity loss; the prepared immobilized enzymes are used for removing the glucose in the isomaltooligosaccharide product at a high removal rate up to over 80%, and are relatively high in repeated utilization ratio; a carrier is cheap and easy to obtain.

Description

technical field [0001] The invention belongs to the application field of immobilized enzymes, in particular to a process method for removing glucose in an isomalto-oligosaccharide product (IMO-500) by immobilizing multiple enzymes. Background technique [0002] Isomaltooligosaccharide is called "bifidus factor". Years of research have shown that bifidobacteria have many health functions, and isomaltooligosaccharide as a bifidofactor has attracted people's attention. Very little isomaltooligosaccharide in nature exists in a free state, so it cannot be directly obtained through the process of extraction, separation and purification. Since isomaltooligosaccharide is mostly used as a component of amylopectin, dextrose and polysaccharides, it is the most direct and economical way to produce isomaltooligosaccharide from these raw materials. Most of the isomaltooligosaccharide products produced in my country are primary products with 50% functional ingredients (IMO-500), which se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H3/04C07H1/06C12N11/14C12N11/08
Inventor 姜艳军辛茜高静
Owner HEBEI UNIV OF TECH
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