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Nattokinase drying protective agent

A drying protective agent, nattokinase technology, applied in the direction of enzyme stabilization, hydrolase, etc., can solve the problems of loss of enzyme activity, no research on drying process and enzyme activity stability, etc.

Active Publication Date: 2018-12-07
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our previous research shows that the production of nattokinase with soybean dregs as a substrate has an enzyme activity comparable to that of soybean substrate, but we have not studied its drying process and enzyme activity stability
The currently reported drying methods for nattokinase are generally vacuum freeze-drying and spray-drying, but the enzyme activity is seriously lost during the drying process.

Method used

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  • Nattokinase drying protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 The preparation of nattokinase crude enzyme liquid

[0023] 1. Activation of strains and preparation of bacterial suspension

[0024] Buy Bacillus natto powder, dissolve it with sterile water, and dilute it to 10 -3 to 10 -6 Different gradient plates were streaked and cultured on LB medium containing 2% agar for 18 hours for microscopic examination. figure 1 , and then pick a single colony into LB liquid medium, activate three generations, then inoculate 3% inoculum in LB liquid medium at 120rpm, and cultivate at 37°C for 12h; the bacterial liquid is placed in a high-speed refrigerated centrifuge at 4°C, 8000r / min centrifuged for 10min; the centrifuged cells were washed twice with sterile water, resuspended, and the concentration of the bacterial suspension was adjusted to 10 7 CFU / mL, as starter culture.

[0025] 2. Fermentation of fresh bean dregs

[0026] Soak the soybeans in distilled water at room temperature for 14 hours, then refine them (soybe...

Embodiment 2

[0029] Embodiment 2 The preparation of nattokinase drying protectant

[0030] Prepare trehalose protective agent, mixed protective agent Ⅰ, mixed protective agent Ⅱ, maltodextrin protective agent, and whey protein powder protective agent for subsequent experiments. The preparation methods are as follows:

[0031] 1. Trehalose protective agent: weigh 18g of trehalose, add 100mL of distilled water and stir until no particles exist;

[0032] 2. Mixed protective agent Ⅰ: Weigh 6g of maltodextrin and 12g of trehalose respectively, add to 100mL of distilled water and stir until no particles exist;

[0033] 3. Mixed protective agent II: Weigh 6g of maltodextrin, 2g of β-cyclodextrin and 10g of whey protein powder into 100mL of distilled water and stir until no particles exist;

[0034] 4. Maltodextrin protective agent: weigh 18g of maltodextrin, add it into 100mL distilled water and stir until no particles exist;

[0035] 5. Protective agent for whey protein powder: Weigh 18g of wh...

Embodiment 3

[0036] Example 3 Nattokinase Crude Enzyme Liquid Drying Experiment

[0037] 1. Optimization of spray drying conditions for nattokinase crude enzyme solution

[0038] According to the nattokinase crude enzyme liquid and various protective agents prepared in Example 2, mix them in a volume ratio of 1:3 (V / V), spray dry them after 10000r / min homogenization for 2 minutes, and set the spray dryer The inlet temperatures were 140, 150, and 160°C, the outlet temperatures were 75°C, the peristaltic pump speeds were 240, 340, and 440mL / h, the fan speed was 70Hz, the firing time was 1s, and the firing interval was 1s. After drying, the enzyme powder was collected for activity determination. The effects of protective agent type, spray drying inlet temperature and spray drying peristaltic pump speed on the activity of nattokinase were investigated.

[0039]Taking the activity of nattokinase as the assessment index, determine the appropriate spray drying conditions for each single factor, ...

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Abstract

The invention discloses a nattokinase drying protective agent. The nattokinase drying protective agent comprises trehalose and maltodextrin, wherein the mass ratio of the trehalose to the maltodextrinis 2:(1-4). A preparation method comprises the steps: respectively weighing the trehalose and the maltodextrin according to the mass ratio being 2:(1-4) of the trehalose to the maltodextrin, adding the trehalose and the maltodextrin to distilled water, and stirring to obtain the nattokinase drying protective agent. A nattokinase drying method comprises the following steps: mixing a nattokinase crude enzyme with the nattokinase drying protective agent according to the volume ratio of 1: (2-5), homogenizing, carrying out spray drying, and collecting nattokinase enzyme powder, wherein the average value of the enzyme activity of produced nattokinase is (210200+ / -19126)FU / g. The nattokinase drying method has the advantages that (1) bean dregs are used as a matrix to produce the nattokinase, the added value of the bean dregs is increased, and the enzyme activity of the nattokinase is comparable to the enzyme activity of the nattokinase produced by using soybeans as the matrix; and (2) the loss of the enzyme activity in the drying process is reduced, and the stability of the nattokinase is improved.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a nattokinase drying protectant. Background technique [0002] In 1987, Dr. Sumi of Japan discovered an enzyme in natto that can effectively dissolve thrombus in the heart and brain—Nattokinase (NK), and since then began the application and research of NK. Nattokinase is extracted from food natto or fermented by Natto bacteria. It is a protein with a molecular weight much smaller than UK, SK, and tPA. It is a serine protease produced by natto during the fermentation process. It can hydrolyze fibrin into Small peptides and amino acids, and has a good thrombolytic ability, and also has a certain effect on lowering blood pressure and preventing cardiovascular and cerebrovascular diseases. Currently, the thrombolytic drugs approved for medical application include: Streptokinase (SK) and Urkinase (Urkinase, UK), etc., but they all have certain defects to a certain extent, s...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N9/96
CPCC12N9/50C12N9/96
Inventor 朴春红于洪良程璐张媛媛王海王玉华刘俊梅于寒松代伟长
Owner JILIN AGRICULTURAL UNIV
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