Method for extracting endoenzyme generated by fungal mycelium liquid submerged fermentation

A technology of liquid submerged fermentation and extraction method, which is applied in the field of intracellular enzyme extraction, which can solve the problems of enzyme activity loss and achieve the effect of less enzyme activity loss and simple operation

Inactive Publication Date: 2015-11-25
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned conventional methods easily cause the loss of enzyme activi

Method used

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  • Method for extracting endoenzyme generated by fungal mycelium liquid submerged fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In this example, Chizhi was used as the strain, which was purchased from the Edible Fungi Engineering Technology Center of Henan Academy of Sciences.

[0038] 1. Separation of mycelium and fermentation broth

[0039] Ferment and cultivate Chizhi according to the above strain culture method, and after 8 days of fermentation, carry out suction filtration to separate the mycelia and fermentation liquid; wherein, the fermentation liquid is measured by colorimetric method for β-glucosidase enzyme activity, and the mycelium is sterile After several washes with water, the extraction of β-glucosidase was performed.

[0040] 2. Extract the crude enzyme solution from the isolated mycelium according to the following method

[0041] (1) Beating treatment

[0042] Add distilled water to the mycelium extracted in step 1, and perform beating treatment in an ice bath (-10~10°C) (the speed of beating is 22000r / min), and the beating time is 1min, and the beating frequency is set to 1 t...

Embodiment 2

[0062] In this example, Grifola frondosa was used as the strain, which was derived from the strains preserved in this laboratory.

[0063] 1. Separation of mycelium and fermentation broth

[0064] Grifola frondosa was fermented and cultivated according to the above strain culture method, and after 8 days of fermentation, suction filtration was carried out to separate the mycelia and the fermentation liquid; wherein, the fermentation liquid was measured by a colorimetric method for β-glucosidase enzyme activity, and the mycelium was After several washes with sterile water, the extraction of β-glucosidase was carried out.

[0065] 2. Extract the crude enzyme solution from the isolated mycelium according to the following method

[0066] Add distilled water to the mycelia extracted in step 1, and perform beating treatment in an ice bath (-10 to 10°C) (the rotating speed during beating is 22000r / min), and after beating for 1min, place the beating in an ice bath (-10 to 10°C). ℃) ...

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Abstract

The invention discloses a method for extracting endoenzyme generated by fungal mycelium liquid submerged fermentation. The method comprises the following steps: separating a fermentation liquor to obtain mycelia, diluting the mycelia with water, and performing beating processing and ultrasonication processing on the diluted mycelia to obtain a crude enzyme. Through combination of the beating processing and the ultrasonication processing, the crude enzyme of endoenzyme generated by fungal mycelium liquid submerged fermentation is obtained, so that the loss of enzyme activity is less. The method provided by the invention is simple in operation, convenient, feasible and suitable for the extraction of enzymes generated by liquid submerged fermentation of various fungal mycelia such as ganoderma, champignon and Grifola frondosa.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for extracting intracellular enzymes produced in submerged fermentation of fungal mycelium liquid. Background technique [0002] Ganoderma lucidum is an extremely precious medicinal fungus with various physiological functions and valuable medicinal value. A variety of biologically active substances contained in the fruiting bodies of Ganoderma lucidum, such as Ganoderma lucidum polysaccharides, Ganoderma lucidum triterpenes, and various enzymes, also exist in the mycelium of liquid culture. [0003] Traditionally, Ganoderma lucidum is obtained through wild collection or artificial cultivation, and its fruiting bodies or spores are used as medicines. However, the formation of the fruiting bodies of Ganoderma lucidum takes a long time and requires high labor intensity. The production of mycelium can be accelerated and the growth cycle can be shortened by li...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/645
CPCC12N9/2445C12Y302/01021
Inventor 何国庆秦可欣刘同杰石彦国徐莉莎
Owner ZHEJIANG UNIV
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