Resin-enzyme composite catalyst and preparation method thereof
A composite catalyst and resin technology, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of low enzyme stability, loss of enzyme activity, and large impact on enzyme structure of composite catalysts, and achieve excellent anti-leaching performance , Strong anti-leaching performance, high operational stability
Examples
Embodiment 1
[0024] Dissolve laccase (molecular weight 69kD, isoelectric point 3~4) in phosphate buffer solution with pH=7.0 to obtain laccase solution, the concentration is 2mg / mL; take 0.20 g macroporous anion exchange resin D201 and mix with 20mL laccase solution , stirred at room temperature for 48 h; then the macroporous anion exchange resin was taken out and the surface of the resin was washed with phosphate buffer solution with pH = 7.0, and then the resin was dried in the shade at room temperature to obtain a resin-enzyme composite catalyst.
[0025] Through Fourier transform infrared spectroscopy (FT-IR) scanning, it was found that compared with D201, the composite catalyst was at 1600-1700, 1220-1330, 600-700 cm -1There are more characteristic absorption peaks of amide groups. The material is shaken in disodium hydrogen phosphate-citric acid buffer solution with pH 3-7 at room temperature for 7 days, and the maximum enzyme loss is <1-15%. Shake in 1M NaCl solution at room temper...
Embodiment 2
[0028] The basic steps are the same as in Example 1, specifically: take 0.20 g of macroporous anion exchange resin D301, mix it with 20 mL of phosphate buffer solution containing 2 mg / mL laccase (molecular weight 69 kD, isoelectric point 3-4), pH = 7.0, and mix in After stirring at room temperature for 48 h, the macroporous anion exchange resin was taken out and the surface of the resin was rinsed with phosphate buffer solution with pH=7.0, and then the resin was dried in the shade at room temperature to obtain a resin-enzyme composite catalyst.
[0029] Through Fourier transform infrared spectroscopy (FT-IR) scanning, it is found that compared with D301, the composite catalyst has more characteristic absorption peaks of amide groups at 1600-1700, 1220-1330, and 600-700 cm-1. When the material is shaken in disodium hydrogen phosphate-citric acid buffer solution with pH 3-7 at room temperature for 7 days, the maximum enzyme loss is <1-10%. Shake in 1M NaCl solution at room temp...
Embodiment 3
[0032] The basic steps are the same as in Example 1, specifically: 0.20 g of macroporous anion exchange resin Amberlite IRA900, mixed with 20 mL of phosphate buffer solution containing 2 mg / mL laccase (molecular weight 69 kD, isoelectric point 3-4), pH = 7.0, in After stirring at room temperature for 48 h, the macroporous anion exchange resin was taken out and the surface of the resin was washed with phosphate buffer solution with pH=7.0, and then the resin was dried in the shade at room temperature to obtain a resin-enzyme composite catalyst. Through Fourier transform infrared spectroscopy (FT-IR) scanning, it was found that compared with Amberlite IRA900, the composite catalyst had more characteristic absorption peaks of amide groups at 1600-1700, 1220-1330, and 600-700 cm-1 . The material is shaken in disodium hydrogen phosphate-citric acid buffer solution with pH 3-7 at room temperature for 7 days, and the maximum enzyme loss is <1-18%. Shake in 1M NaCl solution at room t...
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Description
Claims
Application Information
- IPC
- C12N11/02
- CPC
- C12N9/20; C12N11/08; C12N11/082
- Inventors
- 潘丙才; 张孝林