Resin-enzyme composite catalyst and preparation method thereof

Abstract

The invention discloses a resin-enzyme composite catalyst and a preparation method thereof, belonging to the field of composite materials. The method comprises the following steps: dissolving a target enzyme in a phosphate buffer with pH value being neutral so as to obtain a solution A with concentration of 0.2mg / mL-10mg / mL; selecting a suitable large-hole type ion exchange resin as a carrier according to isoelectric points of an enzyme molecule; mixing and stirring the solution A and the resin carrier at room temperature; and taking out the resin, washing the surface of the resin by utilizing the phosphate buffer and drying the resin to obtain the resin-enzyme composite catalyst. The preparation method provided by the invention has the advantages of simplicity in operation, easiness in control and less loss of enzymatic activity; the fixed-load enzyme composite catalyst prepared by the method provided by the invention ensures that the stability of ordinary enzyme is greatly improved on the basis of maintaining the original catalytic activity of the enzyme molecules, can be applied to a water phase system and has the characteristic of good leaching-resisting property within a wide pH range and under high ionic strength.

Description

technical field [0001] The invention relates to a composite catalyst and a preparation method thereof, in particular to a resin-enzyme composite catalyst and a preparation method thereof. Background technique [0002] Enzyme is a kind of high-efficiency biocatalyst, which has the advantages of fast reaction speed, mild conditions, complete reaction, and specificity for substrates. However, in the common solution reaction system, the enzyme-catalyzed reaction has disadvantages such as difficult recovery and repeated use of the enzyme, poor resistance to the impact of reaction conditions (temperature, pH, salinity, etc.), easy inactivation, and difficult separation of products. This limits the direct promotion and application of enzymes in the field of biocatalysis. In order to overcome these shortcomings, scientists began to immobilize enzymes to prepare carrier-enzyme composite catalysts, and there have been many reports so far. At present, common enzyme composite catalyst...

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Problems solved by technology

It can be seen that these methods have disadvantages such as complicated operation, severe reaction and difficult to control degree, great influence on enzyme structure, and serious loss of enzyme activity.

[0006] In addition, there are also reports of enzymes immobilized on resins by simple adsorption methods. These methods can be summarized as follows: use hydrophobic non-polar macroporous adsorption resins as carriers to immobilize enzymes by adsorption methods. No matter the synthesis or application of this method, Both are limited to organic solvent systems, and have strict requirements on water content, which limits their application in aqueous phase (Acta Bioengineering, 2006, Vol. 22, No. 1, 114; Journal of Henan University of Technology (Natural Science Edition), Volume 28, No. 3, 2007, 68; Agricultural Products Processing Journal, 2010, No. 3, 23); in the water phase, the macroporous adsorption resin was used as the carrier, and the enzyme was immobilized by adsorption, mainly through hydrogen bonds, van der Waals Erli immobilization of enzymes, the stronger the polarity of the resin, the worse the immobilization effect, it is difficult to apply to the aqueous system, and the stability of the enzyme is less improved (Chemical Engineering, 2009, Volume 37, Issue 4, 8); Aminoethylhydroxyethyl ester (DEAE-E / H) is used as the carrier, which mainly immobilizes the enzyme through hydrogen bonding. The composite catalyst prepared by it has little improvement in the stability of the enzyme, and is easily affected by the pH and ionic strength of the solution. The enzyme is leached into the solution, so it is also not suitable for application in aqueous systems (Chemical Reaction Engineering and Technology, 2005, Vol. 21, No. 1, 60)

However, using the strong electrostatic force, the enzyme is immobilized on the highly polar macroporous ion exchange resin by simple impregnation to obtain a composite catalyst with high stability, applicable to aqueous systems, and excellent leaching resistance. No public reports yet