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Physical extracting method for papain

A papain and extraction method technology, applied in the field of physical extraction of papain, can solve the problems of many technical requirements, complicated operation process, high time-consuming and energy consumption, etc., and achieves the effects of flexible combination, high activity and improved purity

Inactive Publication Date: 2007-04-11
谢丹青 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction of papain mainly adopts chemical methods. On the one hand, this method needs to use a large amount of chemical reagents, and the cost is very high;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The physical extraction method of papain in this embodiment comprises the following steps: papaya pulp→freezing→pretreatment→coarse filtration→fine filtration→hot air drying→finished product. Specifically: take fresh papaya pulp, store it in a cold storage, and cool it to -5°C for later use; add 1% antioxidant, 2% bacteriostatic agent, and 0.2% heavy metal chelating agent to the frozen papaya pulp , fully stirred for 30 minutes and cooled to 5°C; coarsely filtered with a 200-mesh screen and a 5μ bag filter to remove impurities and insoluble components in the papaya pulp; then sterilized with a fine filter with a 0.2μ pore size to obtain refined Papaya pulp; finally, the refined papaya pulp is placed in a hot-air drying oven for hot-air drying to obtain solid pale yellow papaya enzyme blocks.

Embodiment 2

[0019] The physical extraction method of papain in this embodiment comprises the following steps: papaya pulp→freezing→pretreatment→coarse filtration→fine filtration→hot air drying→finished product. Specifically: take fresh papaya pulp, store it in a cold storage, and cool it to 0°C for later use; add 2% antioxidant, 1% bacteriostat, and 0.5% heavy metal chelating agent to the frozen papaya pulp, Fully stir for 60 minutes, use a 100-mesh screen and a 10μ bag filter to perform coarse filtration to remove impurities and insoluble components in the papaya pulp; then use a 0.45μ aperture fine filter to sterilize to obtain refined papaya pulp; finally, put The refined papaya pulp is placed in a hot-air drying oven for hot-air drying to obtain solid light yellow papaya enzyme blocks.

Embodiment 3

[0021] The physical extraction method of papain in this embodiment comprises the following steps: papaya pulp→freezing→pretreatment→coarse filtration→fine filtration→hot air drying→finished product. Specifically: take fresh papaya pulp, store it in a cold storage, and cool it to 5°C for later use; add 1.5% antioxidant, 1.5% bacteriostatic agent, and 0.3% heavy metal chelating agent to the frozen papaya pulp. Fully stir for 45 minutes; use a 100-mesh screen and a 5μ bag filter to perform coarse filtration to remove impurities and insoluble components in the papaya pulp; then use a 0.2μ aperture fine filter to sterilize to obtain refined papaya pulp; finally, put The refined papaya pulp is placed in a hot-air drying oven for hot-air drying to obtain solid light yellow papaya enzyme blocks.

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PUM

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Abstract

The physical papain extracting process includes the following steps: forming papaya slurry, freezing, pre-treating, coarse filtering, fine filtering, twice ultrafiltering, and drying to obtain product. Compared with available chemical extraction process, the present invention has the advantages low cost, simple technological process, short drying time, low power consumption, high enzyme activity of the product, high heat stability of the product, etc.

Description

technical field [0001] The invention relates to a method for extracting papain, in particular to a method for extracting papain from papaya by physical means. Background technique [0002] Papain (Papain) is a kind of proteolytic enzyme extracted and purified from papaya pulp by modern biotechnology, belonging to plant protease, referred to as papain. Papain has a very strong hydrolysis ability to animal and plant proteins, esters, amides, etc., and is a broad-spectrum enzyme preparation. Because papain has the characteristics of high enzyme activity, good thermal stability, natural hygiene and safety, and easy use, it can be widely used in food, medicine, daily chemical, feed, leather and textile industries. [0003] At present, the extraction of papain mainly adopts chemical methods. On the one hand, this method needs to use a large amount of chemical reagents, and the cost is high; Contents of the invention [0004] The object of the present invention is to provide a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50
Inventor 刘锋
Owner 谢丹青
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