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Method for fossilizing pectinase by using nanometer silicon material

A technology of immobilizing pectinase and nano-silicon, which is applied in the direction of immobilizing on or in the inorganic carrier, can solve the problems of difficult to realize large-scale commercial production, high production cost and low content, and achieve low cost, The effect of small loss of enzyme activity and uniform particle size distribution

Inactive Publication Date: 2013-07-03
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although pectin oligosaccharides and low-molecular-weight pectin are widely present in citrus pectin, their content is very low. The production cost of direct extraction method is high, and it is difficult to realize large-scale commercial production. Immobilized pectinase combined with enzyme reactor Provides possibility for large-scale preparation of POS and MCP

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] 4. 2g F127, 20g potassium chloride, 250ml deionized water, 50ml hydrochloric acid, mix them into the interlayer beaker, add TMB, after stirring for 3h, add 20ml of ethyl orthosilicate drop by drop, stir for 20h, put in the kettle. Put it in an oven at 110°C for crystallization, and take it out after 24 hours. Dry at 80-100°C for 12 hours. Put into the muffle furnace for calcination. The temperature was raised to 450°C at 5°C / min, kept for 6h, and the nano-silicon material was obtained after cooling. Weigh 0.6g of sodium dodecylsulfonate, add 100ml of double-distilled water, and heat to dissolve; weigh 1.5g of the treated nano-silicon material, add it to the above solution, sonicate for 10min, and soak for 24h. Pour off the unadsorbed sodium dodecylsulfonate solution in the upper layer, add 50ml of 1.0% chitosan solution, and soak for 12h. Centrifuge the nano-silicon material, add 20ml of 2.5% glutaraldehyde solution, cross-link for 4 hours, wash with water and centri...

example 2

[0017] 5.0g F127, 20g potassium chloride, 250ml deionized water, 55ml hydrochloric acid, mix them into the interlayer beaker, add TMB1.0ml, after stirring for 3h, add 22ml of ethyl orthosilicate drop by drop, stir for 20h, put into the kettle. Put it in an oven at 110°C for crystallization, and take it out after 24 hours. Dry at 80-100°C for 12 hours. Put into the muffle furnace for calcination. The temperature was raised to 450°C at 5°C / min, kept for 6h, and the nano-silicon material was obtained after cooling. Weigh 0.7g of sodium dodecylsulfonate, add 100ml of double-distilled water, and heat to dissolve; weigh 1.4g of the treated nano-silicon material, add it to the above solution, sonicate for 12min, and soak for 24h. Pour off the unadsorbed sodium dodecylsulfonate solution in the upper layer, add 50ml of 1.0% chitosan solution, and soak for 12h. Centrifuge the nano-silicon material, add 20ml of 2.2% glutaraldehyde solution, cross-link for 4 hours, wash and centrifuge,...

example 3

[0019] 4.0g F127, 23g potassium chloride, 250ml deionized water, 50ml hydrochloric acid, mix them into the interlayer beaker, add TMB, after stirring for 3h, add 22ml of ethyl orthosilicate drop by drop, stir for 23h, put into the kettle. Put it in an oven at 120°C for crystallization, and take it out after 24 hours. Dry at 80-100°C for 12 hours. Put into the muffle furnace for calcination. The temperature was raised to 450°C at 5°C / min, kept for 6h, and the nano-silicon material was obtained after cooling. Weigh 0.55g of sodium dodecylsulfonate, add 100ml of double-distilled water, and heat to dissolve; weigh 1.5g of the treated nano-silicon material, add it to the above solution, sonicate for 10min, and soak for 24h. Pour off the unadsorbed sodium dodecylsulfonate solution in the upper layer, add 45ml of 1.2% chitosan solution, and soak for 12h. Centrifuge the nano-silicon material, add 25ml of 2.0% glutaraldehyde solution, cross-link for 4 hours, wash with water and cent...

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Abstract

The invention relates to a method for fossilizing pectinase by using nanometer silicon material. The method is characterized in that novel nanometer silicon material is prepared by using a layer-by-layer assembly technique, activation, modification and coating are performed on the material, and efficient immobilization to pectinase is achieved through glutaraldehyde crosslink. The carrier for immobilization, prepared in the invention has the advantage of high protein carrying capacity, and the obtained immobilized pectinase enzyme has the characteristics of small enzyme activity loss and low probability of enzyme dropping, and can be used repeatedly.

Description

technical field [0001] The invention belongs to the technical field of food processing, and relates to a method for immobilizing pectinase by using a novel nanometer silicon material. Background technique [0002] The best quality pectin comes from citrus fruits. The orange peel of citrus fresh food and processed leftovers accounts for about 20% of citrus production and is rich in pectin. At present, only a small number of dried orange peels are used as traditional Chinese medicine or candied oranges, but most of them are not fully utilized and are discharged as garbage. Orange peels are easy to mold and smell, and produce mycotoxins. One of the sources of environmental pollution. As a by-product of citrus processing, orange peel has not been fully utilized, not only causing a huge waste of resources but also causing serious environmental pollution. [0003] The specific degradation products of pectin by endo-polygalacturonic acid were pectin oligosaccharides (POS, DP: 2-1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
Inventor 刘明启金红晓戴贤君
Owner CHINA JILIANG UNIV
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