Catfish feed compound enzyme and preparation method thereof
A feed compound enzyme, catfish technology, applied in the field of feed compound enzyme
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Embodiment 1
[0049] Embodiment 1 raw material preparation
[0050] 1. Preparation of Yeast Culture
[0051] The preparation method of the yeast culture comprises the following steps: take the Saccharomyces cerevisiae tlj2016 slant strain after 1 ring activation, inoculate it into a 500mL Erlenmeyer flask containing 100mL seed medium, and cultivate it at 30°C with a shaker speed of 150r / h. Cultivate under the min condition for 28h to obtain the primary seed liquid; the primary seed liquid is inoculated into a 500mL Erlenmeyer flask containing 100mL fermentation medium at an inoculum size of 8% (v / v) for fermentation and cultivation, and the fermentation time is 28h. The temperature is 30°C, the rotating speed of the shaker is 180r / min, and the secondary seed liquid is obtained; the secondary seed liquid is inserted into a 5L fermenter with 3L fermentation medium for constant temperature fermentation with an 8% (v / v) inoculum, and the temperature is 30°C , the ventilation rate is 5L / min, th...
Embodiment 2
[0071] A catfish feed compound enzyme is mainly prepared from the following raw materials in parts by weight:
[0072] Yeast culture 30 parts, calcium fruit extract 22 parts, wheat germ powder 15 parts, acid xylanase 15 parts, acid protease 15 parts, phytase 15 parts, β-glucanase 8 parts, cellulase 7 parts, amylase 7 parts, β-mannanase 5 parts, pectinase 5 parts, lipase 4 parts, phosphatase 4 parts, palatable agent 3 parts, wormwood seed powder 2 parts;
[0073] The acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, β-mannanase, pectinase, lipase, phosphatase are food grade Enzyme;
[0074] The preparation method includes the following steps: according to the formula, firstly, wormwood seed powder, palatable agent, phosphatase, lipase, pectinase, β-mannanase, amylase, cellulase, β-glucan Add 20% of carbohydrase, phytase, acid protease, acid xylanase, wheat malt powder, yeast culture, and calcium fruit extract into a V-shaped mixing tank and mix evenly fo...
Embodiment 3
[0076] A catfish feed compound enzyme is mainly prepared from the following raw materials in parts by weight:
[0077] Yeast culture 20 parts, calcium fruit extract 15 parts, wheat germ powder 10 parts, acid xylanase 10 parts, acid protease 10 parts, phytase 10 parts, β-glucanase 6 parts, cellulase 5 parts, 5 parts amylase, 3 parts β-mannanase, 3 parts pectinase, 2 parts lipase, 2 parts phosphatase, 1 part palatable agent, 1 part wormwood seed powder;
[0078] The acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, β-mannanase, pectinase, lipase, phosphatase are food grade Enzyme;
[0079] The preparation method includes the following steps: according to the formula, firstly, wormwood seed powder, palatable agent, phosphatase, lipase, pectinase, β-mannanase, amylase, cellulase, β-glucan Add 10% of carbohydrase, phytase, acid protease, acid xylanase, wheat malt powder, yeast culture, and calcium fruit extract into a V-shaped mixing tank and mix evenly for ...
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