Bromelain stabilizer and preparation method and application thereof

Abstract

The invention discloses a bromelain stabilizer and a preparation method and application thereof, and relates to the technical field of biology. The bromelain stabilizer is prepared from the followingcomponents in parts by weight: 0.015 to 0.13 part of reductant, 0.002 to 0.07 part of metal ion chelating agent, 0.01 to 0.09 part of organic carboxylate, 0.05 to 0.15 part of preservative, and 0.001to 0.02 part of vitamin. The bromelain stabilizer has the advantages that the effect of protecting enzyme activity is obvious, the loss of enzyme activity is reduced, the half-life period of enzyme isshortened, the usage amount is small, the cost is low, and the like. The preparation method has the advantages that the operation is simple, and the preparation method is suitable for industrializedproduction; the bromelain stabilizer can be directly added inr the process of storage of enzyme solid powder or production of liquid enzyme, and the application range is broad.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bromelain stabilizer and its preparation method and application. Background technique [0002] Bromelain, also known as bromelain, is a proteolytic enzyme present in pineapple (Anana comosus) plants. Natural plant protease. Bromelain is a light yellow amorphous powder with a molecular weight of about 3300, an isoelectric point of 9.55, a pH of 6.5-7.5, and an optimum pH of 7.1. It is not completely soluble in water, and is also insoluble in organic substances such as ethanol, chloroform and acetone. solvent. [0003] Bromelain belongs to glycoprotein, contains 2.1% sugar components, has 4 N-acetylated hexosamines, is a complex complex composed of sulfhydryl protein and non-protease, and is also a sulfhydryl proteolytic enzyme, which belongs to the mixture. Bromelain exists as a single peptide chain. In the bromelain molecule, the essential groups that play a key role in its cata...

AI Technical Summary

Problems solved by technology

Adopting polysaccharides or polyhydric alcohols etc. as protecting agent generally uses a larger amount, and for pineapple enzyme extract or liquid bromelain solution, its addition amount is larger, although also can be used in a smaller amount for a single salt protecting agent The dose has a certain protective effect, but the protective effect on the enzyme activity is not obvious, and the enzyme activity loss is still relatively fast after storage, especially in the storage of solid powdered bromelain

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