Process for producing collagen sponge, process for producing artificial skin, artificial skin and cell tissue culture substrate

A technology of collagen sponge and manufacturing method, which is applied in the fields of skin transplantation, medical science, prosthesis, etc., can solve the problems of water-permeable adjustment layer wrinkles, lower pass rate, complex process, etc., and achieve the goals of promoting healing, excellent performance, and simplifying the process Effect

Inactive Publication Date: 2007-07-25
GUNZE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are not only complex processes in the above-mentioned existing collagen sponge manufacturing method or artificial skin manufacturing method, but also because cracks and cracks appear on the surface of the collagen sponge layer of the final obtained collagen sponge, artificial skin, etc., Or there are wrinkles on the water-permeable adjustment layer of the artificial skin, and unqualified products appear, resulting in a decrease in the pass rate, etc.

Method used

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  • Process for producing collagen sponge, process for producing artificial skin, artificial skin and cell tissue culture substrate
  • Process for producing collagen sponge, process for producing artificial skin, artificial skin and cell tissue culture substrate
  • Process for producing collagen sponge, process for producing artificial skin, artificial skin and cell tissue culture substrate

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Effect test

Embodiment 1

[0077] Collagen solution (manufactured by Nitta Gelatin, "Atelo-collagen") was used as a raw material to prepare a diluted collagen solution with a concentration of 3 mg / mL and a pH of 3.0 using purified water, 5N acetic acid, and 1N hydrochloric acid.

[0078] 50 g of the obtained diluted collagen solution was poured into a stainless steel frame (11 cm×8.5 cm) for freeze-drying. The stainless steel frame was cooled to -40°C, the collagen foaming solution was frozen, and freeze-dried at 30°C for 24 hours under reduced vacuum (0.01 mmHg). Then, under vacuum and reduced pressure (0.01 mmHg), heat drying was carried out at 105°C for 24 hours to obtain a primary collagen sponge.

[0079] Then, a crosslinking reaction was carried out at 5° C. for 24 hours in a 0.2% by weight glutaraldehyde / acetic acid solution. The obtained cross-linked collagen sponge was sufficiently washed with ion-exchanged water, and then replaced with 15% ethanol aqueous solution.

[0080]Thereafter, it was...

Embodiment 2

[0083] Collagen solution (manufactured by Nitta Gelatin, "Atelo-collagen") was used as a raw material to prepare a diluted collagen solution with a concentration of 3 mg / mL and a pH of 3.0 using purified water, 5N acetic acid, and 1N hydrochloric acid.

[0084] To 50 g of the obtained diluted collagen solution, 0.5 g of chloroform was added. The resulting milky foaming liquid was poured into a stainless steel frame (11 cm×8.5 cm) for freeze-drying. The stainless steel frame was cooled to -40°C, the collagen foaming solution was frozen, and freeze-dried at 30°C for 24 hours under reduced vacuum (0.01 mmHg) to obtain a collagen sponge.

[0085] The obtained collagen sponge was added into an airtight container with a volume of 8000 mL, and 50 mL of the prepared 2% by weight glutaraldehyde / acetic acid solution was added in addition, and the solution was prevented from directly contacting the collagen sponge. The airtight container was left to stand at 40° C. for 3 hours to cross-...

Embodiment 3

[0088] Collagen solution (manufactured by Nitta Gelatin, "Atelo-collagen") was used as a raw material to adjust the collagen concentration to 3 mg / mL and pH 3.0 using purified water, 5N acetic acid, and 1N hydrochloric acid, and then added glutaraldehyde to make The final concentration of glutaraldehyde was 0.05 mM.

[0089] 50 g of the obtained diluted collagen solution containing glutaraldehyde was poured into a stainless steel frame (11 cm×8.5 cm) for freeze-drying. The stainless steel frame was cooled to -40°C, the collagen foaming solution was frozen, and freeze-dried at 30°C for 24 hours under reduced vacuum (0.01 mmHg). Then, under vacuum and reduced pressure (0.01 mmHg), heat drying was carried out at 105° C. for 24 hours to obtain a cross-linked collagen sponge.

[0090] A silicone film with a thickness of 100 μm was pasted on the obtained cross-linked collagen sponge and dried to obtain an artificial skin in which the silicone film was laminated on the collagen spon...

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Abstract

A process for producing a collagen sponge and process for producing an artificial skin, in which a final collagen sponge and artificial skin can be obtained with high yield without occurrence of defectives through simplified steps; and a cell tissue culture substrate and artificial skin utilizing the collagen sponge produced by the above collagen sponge production process. There is provided a process for producing a collagen sponge, comprising using a dilute collagen solution having its pH adjusted with acetic acid.

Description

technical field [0001] The present invention relates to a method for manufacturing a collagen sponge and an artificial skin with a high qualification rate achieved by a simple process, as well as an artificial skin with a collagen sponge and a cell tissue culture substrate manufactured by the method for manufacturing the collagen sponge. Background technique [0002] It is known that collagen sponge is used as artificial skin to be embedded in the affected part of scald etc. to provide a large number of pores (spaces) suitable for the proliferation of fibroblasts produced by its porous structure, and to promote the healing of the affected part by promoting the proliferation of fibroblasts. role. In addition, a product in which a water-permeable regulating layer formed of a silicone film or the like is laminated on the collagen sponge suppresses the evaporation of water from the affected area or prevents the infiltration of microorganisms from the affected area, so it can be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/00A61F2/10
CPCA61L27/24A61L27/60A61L27/56
Inventor 平嗣良
Owner GUNZE LTD
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