Use of taspine for preparing medicament for inhibiting tumor angiogenesis

Abstract

The invention relates to a method for using tasepi alkali to prepare the drug that restrains the growth of tumor vessel, which provides the method for building chicken blast allantois membrane non-small-cell lung cancer A549 tumor transplant cancer model. The invention uses said test model, ELISA, immunity protein label, and Real Time RCR test method to research the effects of tasepi alkali on the CAM vessel structure, the growth of tumor, the shape change and the fresh vessel distribution of tumor, the protein expression of VEGF and bFGF, the gene expressions of VEGF acceptors as flt-1 and flk-1 / KDR, the protein expressions of AKT and Erk1 / 2, and the phosphorylation level, to prove the function of tasepi alkali for restraining the growth of tumor vessel.

Description

technical field

[0001] The invention belongs to the field of compound pharmacological action and molecular biology technology, and specifically relates to the application of taspinine in the preparation of drugs for inhibiting tumor angiogenesis. Background technique

[0002] Angiogenesis refers to the regeneration of new blood vessels on the original vascular bed, which is a complex multifactorial process. Angiogenesis is essential in the occurrence and metastasis of tumors. Studies have found that a series of oncogenes, tumor suppressor genes and growth factors play an important role in regulating tumor angiogenesis. The initiation or conversion of tumor angiogenesis phenotype depends on the balance and coordination of angiogenesis factors and their inhibitors. The complex proteolytic balance system completes the migration, invasion and proliferation of endothelial cells, and finally forms tumor neovascularization. . Blocking angiogenesis is a new means and way to treat ...

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