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Medicinal use of radix rehmanniae total glucosides extractive and preparing method thereof

An extraction method and extract technology, applied in the field of medicine, can solve the problems of reducing the morbidity and mortality of asthma, lack of effective means for asthma, etc.

Inactive Publication Date: 2007-08-15
罗河生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reducing the morbidity and mortality of asthma and improving the prognosis of asthma still lack effective means (Li Minghua et al., Asthma, People's Health Publishing House, 1998, p2)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] [embodiment one] used sample of pharmacological test:

[0045] Sample 1, catalpol 1.0%, rehmanthin D 0.6%, total rehmannia glycosides content calculated as catalpol is 9.5%;

[0046] Sample 2, catalpol 2.5%, rehmanthin D 1.6%, rehmannia total glycosides content calculated as catalpol is 25.0%;

[0047] Sample 3, catalpol 2.3%, rehmanthin D 2.8%, rehmannia total glycosides content calculated as catalpol is 40.7%;

[0048] Sample 4, catalpol 5.2%, rehmanthin D 3.5%, rehmannia total glycosides content calculated as catalpol is 53.5%;

[0049] Sample 5, catalpol 10.5%, rehmanthin D 6.2%, rehmannia total glycosides content calculated as catalpol is 58.7%;

[0050] Sample 6, catalpol 38.2%, rehmanthin D 7.9%, rehmannia total glycosides content calculated as catalpol is 78.9%.

[0051] Asthma induced by ovalbumin aerosol inhalation

[0052] Get 70 guinea pigs of 200-250 g, half male and half male, and inject 0.2 ml of 4% ovalbumin physiological saline solution intramuscula...

Embodiment 2

[0073] [Example two] get the dried root 500g of Rehmannia glutinosa, use 70% methanol 4000ml to heat and reflux extract for 1 hour, incline the extract, and use 70% methanol 4000ml to heat and reflux extract for 1 hour, pour the extract, and The slag was heated and refluxed with 4000ml of 70% methanol to extract for 1 hour, the extract was poured out, and the three extracts were combined. The extract was concentrated in a vacuum below 70°C to 1000ml, and it had no ethanol smell. Add 1000ml of water to remove the precipitate. A good activated carbon (1kg activated carbon) separation column should be washed with water to remove impurities until it is almost colorless, and the sugar reaction test is negative (molish reaction is negative), and it can be eluted with 5000ml of 70% methanol. The eluate was concentrated to dryness below 70°C to obtain 33.4 g of rehmannia total glycosides extract. The total rehmannia glycosides extract was tested, and its chemical components were 2.0% ...

Embodiment 3

[0074][Example three] get 1kg of the dried root of Rehmannia glutinosa, extract by heating under reflux with 8000ml of 50% methanol for 1 hour, incline the extract, and then use 8000ml of 50% methanol for heating and reflux to extract for 1 hour, incline the extract, and The slag was extracted with 8000ml of 50% methanol under heating and reflux for 1 hour, poured the extract, combined three extracts, concentrated the extract in vacuum below 70°C to 1000ml, and had no smell of methanol, added 1000ml of water, removed the precipitate, and processed For a good activated carbon (500g activated carbon, particle size 28 mesh) separation column, wash with water to remove impurities until it is almost colorless, and the sugar reaction test is negative (molish reaction is negative), then wash with 8% dilute methanol until the eluate is almost colorless , and after the sugar reaction test was negative (molish reaction negative), it was eluted with 3000 ml of 70% methanol. The eluate wa...

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Abstract

The invention relates to the application of title total saponins of Rehmannia glutinosa in preparing medicinal composition for treating and preventing asthma and allergic diseases. The title total saponins of Rehmannia glutinosa have anti-inflammatory and anti-allergic effect with low toxicity and adverse effect. The invention also discloses the method for preparing title total saponins of Rehmannia glutinosa. The method comprises subjecting the aqueous solution of Rehmannia glutinosa extract to active carbon adsorption, washing with water or organic solvent with low concentration to remove sugar and other impurity, eluting with organic solvent with high concentration, collecting eluate, and concentrating till dry.

Description

technical field [0001] The invention relates to the field of medicine, and relates to the medical application of total rehmannia glycosides extract for effectively preventing and treating asthma and allergic diseases. The invention is particularly suitable for the treatment and prevention of asthma. It can also be used to treat and prevent allergic rhinitis and allergic skin diseases. Background technique [0002] Bronchial asthma is the most common chronic respiratory disease in the world. It is conservatively estimated that at least 100 million people in the world suffer from asthma. In recent years, its prevalence and mortality have been on the rise. Asthma has become a serious public health problem and has received great attention from all over the world. Therefore, there is a need to provide drugs and methods for effectively treating asthma. [0003] In the past thirty years, a lot of development and research work has been carried out on drugs for the treatment of as...

Claims

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Application Information

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IPC IPC(8): A61K36/804A61P11/02A61P11/06A61P37/08
Inventor 罗河生
Owner 罗河生
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