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Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein

A technology of serum protein and chiral induction, which is applied in the direction of serum albumin, animal/human protein, albumin peptide, etc., to achieve the effect of shortening the reaction time and increasing the action rate

Inactive Publication Date: 2007-08-29
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the existing technique of using a template method to induce the optical activity of achiral cyanine dye aggregates, which can only be induced to produce cyanine dye aggregates with a single optical activity, and is limited by the induced template and the structure of the dye. Deficiency of inability to controllably induce the desired chiral aggregates, thus providing a simple and efficient method for chiral induction of achiral cyanine dye aggregates using human serum albumin, which allows the control of aggregate molecules The chiral inversion of , to obtain the desired optical activity of chiral aggregates

Method used

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  • Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein
  • Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein
  • Method for conducting chiral induction to non-chiral cyanine dye aggregate using human serum protein

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Effect test

Embodiment 1

[0025] Add 0.5ml 80.0μM phosphate buffer solution (pH8.0) of achiral cyanine dye aggregates to a 10.00ml volumetric flask, then add 0.4ml 50.0μM phosphate buffer solution (pH8.0) of human serum albumin, in the mixture The molar concentration ratio of the human serum protein to the achiral cyanine dye aggregate is 0.5:1; then the mixed solution is diluted with phosphate buffer solution to a concentration of 5 μM of the achiral cyanine dye aggregate; after the mixed solution is shaken, After reacting in the dark for 12 hours, the induced cyanine dye aggregates were obtained. After UV-Vis absorption spectrum and circular dichroism analysis, as shown in Figure 2, the absorption peak (686nm) of the dye aggregates on the absorption spectrum decreased and appeared in the short wave direction. The characteristic peak (615nm) of the dye monomer, the signal of the aggregate appears on the circular dichroism spectrum, indicating that the dye aggregate produces photoactivity under the acti...

Embodiment 2

[0029] Add 0.5ml 80.0μM phosphate buffer solution (pH6.0) of achiral cyanine dye aggregates to a 10.00ml volumetric flask, then add 1.0ml 50.0μM phosphate buffer solution (pH6.0) of human serum albumin, in the mixture The molar concentration ratio of the human serum protein to the achiral cyanine dye aggregate is 1.125:1; then the mixed solution is diluted with phosphate buffer solution to a concentration of 5 μM of the achiral cyanine dye aggregate; after the mixed solution is shaken, After reacting in the dark for 9 hours, the induced cyanine dye aggregates were obtained. After UV-Vis absorption spectrum and circular dichroism analysis, the absorption peak (686nm) of the dye aggregates in the absorption spectrum decreased, and the characteristic peak of the dye monomer appeared in the short wave direction. (615nm), the signals of aggregates appear on the circular dichroism spectrum, indicating that the dye aggregates produce photoactivity under the action of human serum album...

Embodiment 3

[0031] Add 0.5ml 80.0μM phosphate buffer solution (pH7.0) of achiral cyanine dye aggregates in a 10.00ml volumetric flask, then add 1.6ml 50.0μM phosphate buffer solution (pH7.0) of human serum albumin, the mixture The molar concentration ratio of the human serum protein to the achiral cyanine dye aggregate is 2:1; then the mixed solution is diluted with phosphate buffer solution to a concentration of 5 μM of the achiral cyanine dye aggregate; after the mixed solution is shaken well, After 15 hours of light-shielding reaction, the induced cyanine dye aggregates were obtained. After UV-Vis absorption spectrum and circular dichroism analysis, as shown in Figure 3, the absorption peak (686nm) of the dye aggregates on the absorption spectrum was significantly reduced. A strong characteristic peak (615nm) of the dye monomer appears, and aggregate signals appear on the circular dichroism spectrum, indicating that the dye aggregate produces photoactivity under the action of human seru...

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Abstract

The invention relates to a method to take chiral induction to non-chiral cyanine dye aggregate by using human albumin that includes the following steps: dissolving non-chiral cyanine dye and human albumin aggregate into phosphoric acid to form 80um and 50um solution, mixing the solution to make the mol ratio to 0.5-5:1; diluting the phosphoric acid solution to 5um, taking non-light reaction for 9-15 hours to gain cyanine dye aggregate, taking analysis by ultraviolet visible absorption spectrum and circle dichromatism spectral, the aggregate has chirality and chirality reversing. The invention could adjust the optical rotation of dye aggregate solution.

Description

technical field [0001] The invention relates to a method for chiral induction of non-chiral cyanine dye aggregates by using human serum albumin. Background technique [0002] The chemical name of the cyanine dye is 3,3'-disulfopropyl-4,5,4',5'-dibenzo-9-phenyl-thiacarbocyanine dye-triethylamine salt (C 43 h 47 N 3 o 6 S 4 ), its structural formula is as follows: [0003] [0004] It has long been discovered that cyanine dyes have unique light-sensitive properties, and they are used as developers, photosensitizers, nonlinear optical materials, etc. Recently, it has been discovered that cyanine dyes also have the effect of inhibiting cancer cells, and can be used to prepare drugs for treating cancer. Whether it is used as an optical material in photoresponsive devices or as a drug, specific optical isomers are required. Usually, in aqueous solution, cyanine dye molecules self-assemble to form aggregates through non-covalent bond forces. Aggregates assembled from ach...

Claims

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Application Information

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IPC IPC(8): C09B23/04C09B67/10C07K14/765
Inventor 张亚周杜鸿雁向俊峰唐亚林徐广智
Owner INST OF CHEM CHINESE ACAD OF SCI
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