Process of measuring carbonyl compound content in oil and grease
A standard substance, isopropanol technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of long time, cumbersome operation, large amount of solvent, etc.
Inactive Publication Date: 2007-10-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology
This method uses a large amount of solvent and is cumbersome to operate, and takes a long time, etc. It is not suitable for on-site use in oil and fat food factories.
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[0008] Weigh 0.04 to 1.0 g of the oil sample and put it into a 10 ml volumetric flask, and dilute to volume with isopropanol (or n-butanol) solution. Extract 1mL sample solution into a 15ml centrifuge tube, add 1ml 2,4-dinitrophenylhydrazine solution (3.5% HCL), cover with a stopper, shake well, and heat in a water bath at 40°C for 20min. After the reaction, it was taken out and cooled to room temperature with running water, and 8 ml of 4% KOH isopropanol solution was added to develop the color. The test tube was centrifuged at 3000 rpm / min for 5 min, and the supernatant was taken to measure its absorbance at 410-425 nm.
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Oxidized and degraded oil and grease contain great amount of harmful carbonyl compound, so that carbonyl compound content in oil and grease becomes one of the important safety indexes. The present invention provides safe, accurate and fast process of measuring carbonyl compound content in oil and grease. The process adopts high safety n-butanol as solvent, and includes the reaction of carbonyl compound in oil and grease and 2, 4-dinitro phenyl hydrazine in acid condition to produce 2, 4- dinitro phenyl hydrazone, the reaction of 2, 4- dinitro phenyl hydrazone in alkaline condition to produce red quinine, centrifugal separation to obtain supernatant, with absorption spectrum in 410-425 nm, and colorimetric quantitative analysis with 2, 4-decadienoic aldehyde as standard matter.
Description
technical field [0001] When fats (animal oil, vegetable oil, mineral oil) are oxidized and spoiled, a large amount of aliphatic and aromatic carbonyl compounds are produced. The amount of carbonyl compounds contained in fats and oils has become one of the important safety indicators of edible fats and oils. Background technique [0002] The previous method of measuring the content of total carbonyl compounds used the toxic solvent benzene, dissolved the oil sample and 2,4-dinitrophenylhydrazine in the benzene solvent and heated the reaction at 60 degrees for 30 minutes, and the color development under alkaline conditions After the reaction, the total carbonyl compound content was determined by colorimetric method with n-hexanal as the standard substance by absorbance at 440nm. This method uses a large amount of solvent, is cumbersome to operate, takes a long time, etc., and is not suitable for on-site use in oil and fat food factories. Contents of the invention [0003] ...
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Inventor 李昌模
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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