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Process of preparing human serum base matter containing no target protein

A protein and human serum technology, applied in the field of reference material preparation for medical testing, can solve problems such as insufficient understanding, unclear composition, content and mechanism of action, and inability to represent serum content, so as to reduce matrix effects and ensure detection. the effect of the result

Inactive Publication Date: 2007-10-03
TSINGHUA UNIV +1
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Problems solved by technology

However, human serum is a very complex mixture, which may have hundreds of components. At present, its exact composition, content and mechanism of action are still unclear, especially for polypeptide growth factors, hormones and lipids. know
Supplements therefore do not represent true serum content

Method used

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Examples

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Embodiment Construction

[0012] The present invention will be described in detail below in conjunction with specific embodiment:

[0013] 1. Analysis of mixed serum from normal people: take some mixed serum from normal people, and analyze the physical and chemical properties of the serum after centrifugation: such as serum viscosity, pH value, protein content, etc. The protein content analysis includes serum total protein (biuret method), albumin (bromocresol green method), globulin (the difference between total protein and albumin according to conventional methods), common special proteins such as: transferrin protein, prealbumin, immunoglobulin A / G / M, complement C 3 / C 4 And the target protein, etc. (immunoturbidimetric analysis). The analytical instrument is a fully automatic biochemical analyzer (Hitachi 7080).

[0014] 2. Preparation of solid immune media containing target protein-specific antibodies and immunomagnetic beads:

[0015] 2.1 Preparation of solid immune medium: Mix a certain amou...

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Abstract

The process of preparing human serum base matter containing no target protein based on immunological principle includes making polyclonal or monoclonal antibody combined to different mediums and resisting target protein react with target protein in human serum to form antigen-antibody complex, and eliminating the complex in different methods to obtain human serum base matter containing no target protein. The present invention can make the base matter for preparing standard matter identical with the base matter in the detected sample and reduce the matrix effect caused by the dissimilar base matter to ensure accurate detection result.

Description

technical field [0001] The invention relates to a method for preparing a target protein-free human serum matrix material mainly used in medical testing, and belongs to the technical field of standard material preparation in medical testing. Background technique: [0002] Standard substances refer to materials and substances that have one or more sufficiently uniform and well-defined characteristic values ​​for calibrating equipment, evaluating measurement methods, or assigning values ​​to materials. The development trend of reference material development includes two directions: one is from traditional fields to emerging fields such as biology and clinical practice; the other is the bipolar development of high-purity and complex matrices. [0003] Protein standard substances are important standard substances in the fields of biology and clinical practice. Currently, there are no protein standard substances suitable for medical testing in my country's standard substance resea...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N33/68G01N33/577
Inventor 胡卫江周海梦孟凡国李海龙
Owner TSINGHUA UNIV
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