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Fluid micro-flux measuring method

A measurement method and micro-flow technology, applied in liquid/fluid solid measurement, measurement of flow/mass flow, measurement devices, etc., can solve the problems of precision and complexity, difficult to meet, and low accuracy of micro/nano upgrade micro-flow measurement. The effect of high measurement accuracy, low cost and broad application prospects

Inactive Publication Date: 2007-10-17
FUDAN UNIV
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Problems solved by technology

Both of these measurement technologies require sophisticated and complex processing technology, and the accuracy of micro / nano-level micro-flow measurement is not high, so it is difficult to meet the needs of fluid micro-flow measurement in the field of microfluidics

Method used

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  • Fluid micro-flux measuring method

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Experimental program
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Effect test

Embodiment

[0017] 1. Select a section of fused silica capillary with an inner diameter of 75 μm, and wash the inner wall with dichloromethane and blow dry with nitrogen.

[0018] 2. The section of capillary tube is regularly wound into a tight spiral shape with a length of 39.70 cm per turn, and a scale is attached to the spiral surface.

[0019] 3. Fill the capillary tube with deionized water, and then inject 1cm Hg column, and then inject deionized water to seal this section of mercury, and ensure that there are no bubbles between the three.

[0020] 4. The flowmeter is connected to the end of the microfluidic liquid chromatography column through a two-way joint, and the distance that the mercury column moves in the microchannel within a certain period of time is measured, combined with the cross-sectional area data of the microchannel, and it can be calculated by the following formula Flow data:

[0021] F=S×L / t (1)

[0022] In the formula, F is the flow rate, S=4.4×10 -9 m...

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Abstract

The invention which belongs to flow measurement technical field uses microchannel with cross section between 1x10-10 and 1x10-6m2 and obtains the flow data by calculation by measuring the distance of label moves in the microchannel in a certain time. The invention solves the problem of liquid flow measurement of micro / nano liter, the microflow measurement device which is designed with simple process, low costs, high measurement precision can be widely used in the field of biochemical analysis, chiral separation, neural science, protein and peptide analysis using the method of chip laboratory, microcolumn high performance liquid chromatography, etc.

Description

Technical field [0001] The invention belongs to the technical field of fluid flow measurement, and in particular relates to a method for fluid micro-flow measurement based on a constant-volume microchannel. technical background [0002] The progress of micro-electromechanical processing technology (MEMS) has greatly promoted the development of the miniaturization of analytical instruments. Scholars at home and abroad agree that in the future the development direction of the entire analytical instrument is mainly miniaturization, integration, and intelligence on this basis. [0003] Microfluidic technology (Microfluidic) is a high-tech developed in the 1990s and has broad application prospects in the miniaturization and integration of analytical instruments. How to accurately measure fluid flow in the field of microfluidics is a very critical technology. The current commonly used flow measurement methods can be basically divided into two categories according to the principle: 1. B...

Claims

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Application Information

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IPC IPC(8): G01F1/00G01F1/704
Inventor 张祥民陶芊吴谦纪杰
Owner FUDAN UNIV
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