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Method for setting acetic-acid gastric ulcer disease recurrence model

A technology for disease recurrence and gastric ulcer, applied in the biological field, can solve difficult-to-prevent and easy-to-relapse problems

Inactive Publication Date: 2007-11-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disease mostly occurs in the stomach and duodenum, and is characterized by easy recurrence and difficult prevention

Method used

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  • Method for setting acetic-acid gastric ulcer disease recurrence model
  • Method for setting acetic-acid gastric ulcer disease recurrence model
  • Method for setting acetic-acid gastric ulcer disease recurrence model

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0056] Example 1 Establishment of rat model of acetic acid gastric ulcer and natural healing of the ulcer

[0057] Male Wistar rats were used in the experiment. After being anesthetized with ether, they were fixed supine on the rat operating table, and the abdomen was disinfected. A 1.5 cm incision was made in the lower abdomen of the xiphoid process. Inject 75 microliters of 100% (VN) acetic acid into the plastic tube at the junction of the stomach body and the pylorus, and immediately wipe off the acetic acid in the plastic tube with a cotton swab after 25 seconds. Layer, close the abdominal cavity, both to replicate the rat model of acetic acid ulcer.

[0058] After the acetic acid ulcer is made, it will heal naturally. The ulcer area seen by the naked eye is about 27mm on the 3rd day after the ulcer is made. 2 (see Figure 1), the arrow in the figure points to the ulcer; typical ulcer structures appear under the microscope (see Figure 2). On the 15th day, the ulcer area v...

example 2

[0059] Example 2 Aspirin was used to induce recurrence of ulcer after natural healing

[0060] 1. Aspirin group: on the 25th to 54th day after the acetic acid ulcer was made, the rats were fasted for 4 hours every day, and the rats were given aspirin (40 mg / kg body weight / day) by intragastric administration, and then fasted for 1 day. Hour.

[0061] 2. Fasting control group: On the 25th-54th day after the acetic acid ulcer was made, the rats were fasted for 5 hours every day.

[0062] 3. Saline control group: On the 25th-54th day after the acetic acid ulcer was made, the rats were fasted for 4 hours every day, and the rats were given normal saline (4ml / kg body weight / day) by intragastric administration, and then fasted again. Eat for 1 hour.

[0063] On the 35th, 45th, and 55th day after the ulcer was made, 8 rats were randomly killed in each group with excessive ether, and the recurrent ulcer area was measured (see Table 1). The data in Table 1 are expressed as mean ± stand...

example 3

[0066] Example 3 Determination of the number of dilated glands

[0067] The inner diameter of the lumen of the gland is greater than 8 μm (about 3 times the inner diameter of the normal lumen) as the criterion for judging cystic dilatation of the gland. Under the magnification of 400 times under the light microscope, the number of cystic dilated glands in the regenerated mucosa was measured in H-E stained sections. On the 55th day after the ulcer was made, the number of dilated glands in the aspirin group was more than that in the fasting control group and the saline control group (Fig. 13, P<0.01).

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Abstract

The present invention provides a method for creating rat acetic acid gastric ulcer recurrence model. Said method includes the following steps: creating rat acetic acid gastric ulcer and ulcer natural healing model, and using aspirin to induce the naturally-heated ulcer to be recurred, measuring the change of physiological and biochemical indexes in the process of rat ulcer recurrence so as to confirm that the recurrence model of gastric ulcer is created. Said model not only can be used in the research of clinical peptic ulcer recurrence mechanism, but also can be used for screening the medicines for clinically curing peptic ulcer and medicines for resisting ulcer recurrence.

Description

technical field [0001] The invention belongs to biological technology, and relates to a method for establishing an animal acetic acid gastric ulcer disease recurrence model. The established animal acetic acid gastric ulcer disease recurrence model can be applied to the screening of anti-ulcer recurrence drugs, and can also be applied to the research on the mechanism of peptic ulcer recurrence . Background technique [0002] Peptic ulcer refers to a localized tissue defect disease that occurs in the mucosa, submucosa, even muscular layer and serosa in the digestive tract bathed by gastric juice, gastric acid and pepsin under the influence of various factors. It is a common disease. , frequently-occurring disease. The disease mostly occurs in the stomach and duodenum, and is characterized by easy recurrence and difficult prevention. Therefore, establishing an animal model of peptic ulcer recurrence can be used to screen highly effective anti-ulcer recurrence drugs, study the...

Claims

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Application Information

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IPC IPC(8): A61K49/00G01N33/48
Inventor 王金福王国忠
Owner ZHEJIANG UNIV
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