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Enzyme labeled chip method capable of in situ detecting multiple ordinary small molecule drugs

A chip and microplate technology, applied in the field of biochips, can solve problems such as difficulty in preparing highly specific anti-small molecule antibodies

Inactive Publication Date: 2007-11-28
杜宏武 +2
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AI Technical Summary

Problems solved by technology

In the small molecule drug-macromolecule carrier linker, whether the site of the small molecule itself can be effectively exposed is a big problem, which has not been well resolved so far, so the preparation of highly specific anti-small molecule antibodies is very difficult

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0027] The actual detection effect of this method is verified by the detection of hair samples from heroin addicts.

[0028] We conducted preliminary experiments on the hair of dozens of heroin poisoning victims. On the one hand, we optimized the extraction and separation conditions, and explored the detection conditions of GC / MS; Antigen spotting concentration, etc., have been detected by the enzyme-labeled chip technology to detect the heroin metabolite morphine in the hair, blood and urine of the poisoned dead, and basically mastered the key technology of this method. On the basis of this work, it is possible to develop a new method that can simultaneously detect a variety of common small molecule drugs by continuously increasing the number of detection varieties, selecting appropriate antibody pairs, and reducing cross-reactions. The enzyme-labeled chip technology has the characteristics of simple sample pretreatment, sensitivity and rapidity, and high throughput. It can b...

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PUM

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Abstract

The invention relates to an enzyme-mark chip technology fro measuring multiple common small molecular drugs in situ at the same time, belonging to the biochip technology field. The invention combines all advantages of biochip technology and enzyme immunoassay method skillfully and develops a new enzyme-mark chip technology. It can measure multiple common drugs in blood and urine so on with many people and in high throughput so as to satisfy requirements of drug case identification, drug control and anti drug. At the same time a new doping detection technology is tried to be added for Beijing Olympic Games in 2008. The invention prepares enzyme-mark chip by adopting polymer film as matrix material and spotting sample by manipulator. Then by special secondary antibody and enzymatic reaction classification and concentration of drug in measuring sample are judged according to gray value of positive point. Measuring result of invention can be contrasted and validated by gc-ms method and hplc-ms method so on.

Description

technical field [0001] The invention relates to an enzyme-link immunosorbent assay array (ELISA-array for short) technology capable of simultaneously detecting multiple common small molecule drugs in situ, belonging to the technical field of biochips. Background technique [0002] The original meaning of Dope is an opioid narcotic mixture used for racing horses. The definition of doping in today's sports world is: a general term for certain drugs or physiological substances that can enhance or assist in enhancing one's own physical fitness or control ability, so as to improve competition performance. The usual practice in the world for judging whether to use doping is: if an athlete ingests prohibited drugs in any form during a competition, or uses an abnormal amount of physiological substances in an abnormal way, this artificially illegal increase The practice of athletic performance is known as doping. The situation of doping abuse in the world today is so serious that e...

Claims

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Application Information

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IPC IPC(8): G01N33/94G01N33/547
Inventor 杜宏武魏玉芝李绍旭
Owner 杜宏武
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