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Method for preparing total salvianolic acid and the application in the preventing and treating of diabetes and the complication thereof

A technology of total phenolic acid of salvia miltiorrhiza and diabetes, applied in the field of preparation of total phenolic acid of water-soluble extract of salvia miltiorrhiza, can solve the problems of lack of comparative data, insignificant treatment effect, etc. Improve the effect of abnormal free radical metabolism

Active Publication Date: 2008-01-02
CHIATAI QINGCHUNBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, most of the Danshen preparations currently used for diabetic complications belong to traditional Chinese medicine compound preparations, double salt preparations or the initial extract of Danshen extract and a certain monomer, such as Salvianolic acid A, salvianolic acid B, the therapeutic effect is not obvious; at the same time, there is a lack of comparative data of commonly used drugs in clinical practice

Method used

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  • Method for preparing total salvianolic acid and the application in the preventing and treating of diabetes and the complication thereof
  • Method for preparing total salvianolic acid and the application in the preventing and treating of diabetes and the complication thereof
  • Method for preparing total salvianolic acid and the application in the preventing and treating of diabetes and the complication thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 : Preparation of Salvianolic Acids:

[0047] 1. Water extraction (warm soaking method): 1000g of Danshen coarse powder, add 8 times the amount of water and warm soak at 80°C for 3 times, each time for 1.0 hour, and filter.

[0048] 2. Purification of total phenolic acids by column chromatography: centrifuge the combined filtrates (4000 rpm) for 10 minutes, take the supernatant and pass it through a D1400 macroporous adsorption resin column, wash with water until the sugar detection reaction is negative, then use 20% ethanol Elute with 10 column volumes.

[0049] (3) elute with 50% ethanol, collect the eluate, analyze and measure the content of salvianolic acid A and salvianolic acid B in the eluent simultaneously, when salvianolic acid A: salvianolic acid B=0.90~1.10: At 1 o'clock, collect this part of the eluate, and concentrate the eluate under reduced pressure at 60°C until there is no alcohol smell;

[0050] (4) adjust the pH value, organic solvent extr...

Embodiment 2

[0061] It is basically the same as Example 1, except that the extraction process is reflux extraction with 60% ethanol aqueous solution.

[0062] The specific method is as follows: add 10 times the amount of 60% ethanol to the coarse powder of salvia miltiorrhiza and extract under reflux for 3 times, each time for 1.5 hours, and filter.

[0063] The ratio of salvianolic acid A and salvianolic acid B in the obtained salvianolic acid is 0.96:1

Embodiment 3

[0065] It is basically the same as in Example 1, except that 20% ethanol is used to elute 8 times the column volume when purifying the total phenolic acids by column chromatography. The ratio of salvianolic acid A and salvianolic acid B in the obtained salvianolic acid is 0.96:1

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Abstract

The invention relates to a method for preparing salvia miltiorrhizae phenolic acid and its new application in medicine production field. The method comprises following steps: adding water or alcohol into salvia miltiorrhizae for extraction, purifying phenolic acid through column chromatography and purification; washing with alcohol solution, condensing washing solution; adjusting pH, extracting with organic solvent, condensing and drying, getting salvia miltiorrhizae phenolic acid with salvia miltiorrhizae phenolic acid A and B content being above 90%. It is proven by medical effect and pharmacological research that the salvia miltiorrhizae phenolic acid can be used to prepare medicine that treats type-2 diabetes and its complication, including diabetes kidney disease, hepatic disease, and blood rheological property change caused by diabetes. It is tested that the effect of salvia miltiorrhizae phenolic acid and its preparation is strong, and they are safe medicine.

Description

technical field [0001] The invention relates to a preparation method of a water-soluble extract salvianolic acid and its new application in the field of pharmacy. Specifically, it is the preparation method of salvia salvia phenolic acid, the water-soluble extract of salvia miltiorrhiza, and the application of the extract in preventing and treating diabetes and its complications. Background technique [0002] Salvia (Radix Salviae), also known as blood root, red ginseng, blood ginseng, red root. The English name Root of Dan-Shen is the dry root and rhizome of Salvia miltiorrhiza Bunge. It is a famous traditional Chinese medicine for promoting blood circulation and removing blood stasis. Shennong Herbal Classic is listed as the top grade. [0003] The chemical composition of Danshen is mainly divided into two types: fat-soluble and water-soluble. The water-soluble components are mainly Danshensu, protocatechualdehyde, rosmarinic acid, shikonic acid, salvianolic acid A, B, C,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61K31/343A61P1/16A61P3/10A61P7/00A61P13/12A61K125/00
Inventor 黎豫杭王木兰柴建国
Owner CHIATAI QINGCHUNBAO PHARMA
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