Fugu rubripes breeding method

A red-finned pufferfish, genetic distance technology, applied in climate change adaptation, animal husbandry, fish farming, etc., can solve the problems of declining economic traits and production performance, unclear genetic composition of breeding objects, easy to produce inbreeding, etc. , to avoid the decline of economic traits and production performance, take into account the optimal breeding and resource utilization, and maximize the utilization of broodstock resources

Inactive Publication Date: 2008-03-05
大连天正实业有限公司
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Problems solved by technology

[0004] With the traditional breeding methods mentioned above, since the genetic composition of the selected breeding object is not clear before breeding, it is easy to produce inbreeding, and cause the common genetic decline of economic traits and production performance.
In recent years, molecular marker-assisted breeding technology has been applied more and more in the genetic selection of aquatic animals. However, most of them only use molecular markers to analyze and describe the genetic characteristics of bred species, and there is no use of molecular markers to detect parents. Genetic composition to guide the complete technical program of breed breeding
Hao Jun et al. conducted a preliminary analysis of the microsatellite DNA polymorphism of the red-finned puffer (Primary analysis of the microsatellite DNA polymorphism of the red-finned puffer, (Journal of Shanghai Fisheries University, 2006.1, Vol. 15, No. 1: 21-24 ), but did not specifically point out the specific technical scheme of using the analysis results of the microsatellite polymorphic markers to guide the parents to pair 1 to 1 breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Selection of redfin puffer

[0048] The red-finned fugu used in the experiment was collected from Dalian Tianzheng Industrial Co., Ltd.: 5 parental individuals of red-finned fugu were taken in April 2005, and 30 individuals were taken from the three offspring families. In April 2006, 90 individuals with 3 offspring families were mixed. And measure their body length and weight. Among them, the parents of the red-finned puffer were collected from Nanyan County (No. 2) in Hebei, Xiaohaidong (No. 4) in Hebei, and Zhuanghe (No. 1, No. 3, and No. 5) in Liaoning. Family 1, No. 3 and No. 4 will be paired together, and the offspring will be Family No. 2, and No. 1 and No. 2 will be paired together, and the offspring will be Family No. 3.

[0049] (2) Extraction of genomic DNA

[0050] (1) Components of DNA Extraction Solution

[0051] EDTA (pH8.0) 10mmol / L

[0052] Sodium Lauryl Sarcosinate 0.5%

[0053] Proteinase K 200μg / mL

[0054] (2) Extraction of sample DNA

[00...

Embodiment 2

[0088] The specific experimental steps of this embodiment are the same as in Example 1. According to the microsatellite DNA marker detection results and the analysis of the similarity and genetic distance between individuals, the fry are paired and divided into 3 groups. The expected heterogeneity of these 3 groups of fry The degree of fit is: Group 1>Group 2>Group 3, and the growth status of the fry 6 months before feeding is shown in Figures 1 and 2.

[0089] It can be seen from the results in the accompanying drawings that the average body length and average body weight are both group 1 > group 2 > group 3, which is consistent with the order of expected heterozygosity of fry.

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Abstract

The present invention provides a method for guiding genetic breading of red-fin blowfish by utilizing microsatellite pleiomorphy analysis result. Said method includes the following steps: (1), according to phenotypic character selecting parental red-fin blowfish for propagation; (2), utilizing microsatellite marker to detect parental DNA, statistically analyzing and calculating individual similarity and genetic distance; (3), according to the individual genetic distance guiding parental one-to-one grouping propagation; and (4), making progeny breeding proparation and cultivation.

Description

technical field [0001] The invention relates to a breeding method of aquaculture fish - red-finned puffer (Takifugu Rubipes), in particular to a method for genetic selection by using microsatellite DNA polymorphism markers. Background technique [0002] Red fin puffer (Takifugu Rubipes) is distributed between the south of Hokkaido, Japan and the north of Taiwan Province of China. It is a very delicious marine fish. In recent years, due to resource depletion, the catch has decreased, and breeding and releasing have gradually become an important means of maintaining resource abundance. In recent years, large-scale farming has begun in northern China, including Dalian in Liaoning, Yantai in Shandong, and Qinhuangdao in Hebei. Most of the products are exported to Japan. They are welcomed by the international market, especially the Japanese market. They are high-end aquatic products that earn foreign exchange through export in my country. one. Japan's research on the breeding te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C12Q1/68
CPCY02A40/81
Inventor 孟雪松孙效文刘进京张涛袁旭
Owner 大连天正实业有限公司
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