Method for detecting total foam protein component and content of beer barley and malt
A barley and malt technology, used in the preparation of test samples, measurement of color/spectral properties, biological testing, etc., can solve problems such as inability to occupy a place and low quality
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[0041] Four varieties of barley were germinated under the same conditions, namely Schooner (Australia), Gairdner (Canada), Ganpi No. 2 (Xinjiang), and Ganpi No. 3 (Inner Mongolia).
[0042] Germination conditions: pH 6.8 for soaking and spraying water, 16°C, dark room, ventilation.
[0043] Germination time: Soak wheat for 24 hours, germinate for 96 hours, a total of 120 hours.
[0044] Sampling is taken every 24 hours, and the original wheat sample is recorded as 0 hour of germination. There are a total of 24 samples, which are recorded as Schooner S0~S120, Gardner G0~G120, Gan Beer No. 2 X0~X120, Gan Beer No. 3 M0~M120.
[0045] Extraction buffer pH7.5, Tris-Cl50mmol L -1 , NaCl10mmol·L -1 , DTT1mmol-L -1 , EDTA·2Na 1mmol·L -1 , and the balance is water.
[0046] Crush the above 24 samples respectively, weigh 1g each (the absolute dry weight after deducting water), put it in a centrifuge tube, add 3ml of 4°C extraction buffer, shake well, and extract at 4°C for 1 hour,...
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