SCIRR69 gene, regulation BDNF transcription and application in biomedicine research, biological therapeutic research and invention

A gene and gene encoding technology, applied in the field of transcription factors and their coding sequences, can solve the problems of unclear molecular mechanism and intricate interaction, etc., and achieve the effect of clear protein structure and easy preparation

Inactive Publication Date: 2008-05-07
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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Problems solved by technology

Since the repair process of spinal cord injury involves quite a few genes, the interactions between them are intricate. Although the understanding of the molecular mechanism of injury repair has made great progress, the molecular mechanism of spinal cord injury repair is still far from clear [7-9]

Method used

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  • SCIRR69 gene, regulation BDNF transcription and application in biomedicine research, biological therapeutic research and invention
  • SCIRR69 gene, regulation BDNF transcription and application in biomedicine research, biological therapeutic research and invention
  • SCIRR69 gene, regulation BDNF transcription and application in biomedicine research, biological therapeutic research and invention

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Embodiment Construction

[0049] The invention provides a SCIRR 69 gene, regulation of BDNF transcription and application in biomedical research and research and development of biotherapeutic drugs. The present invention is further illustrated in the following examples, which do not limit the scope of the present invention.

[0050] Acquisition and analysis of the full-length sequence of SCIRR 69 gene

[0051] (1) Obtain the full-length cDNA of SCIRR gene 69 by Race technology. (See figure 1 )

[0052] (2) Gene sequencing and splicing: 5'-RACE-PCR has a single band at 1.2kb, and the sequencing result of this band can be spliced ​​together with EST-69, so we successfully obtained the 5' end sequence of SCIRR-69 ; 3'-RACE-PCR mainly has two amplification bands, respectively 1.5kb and 1.95kb ( figure 1 A). Sequencing results show that the sequence of the 1.95Kb band contains the sequence of the 1.5Kb band, both of which can be spliced ​​together with EST-69, and both sequences have a polyA structur...

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Abstract

The invention relates to a SCIRR 69 gene, an adjusting BDNF transcription and biomedical research and application in biological therapeutic drug development, belonging to gene engineering field, detailed speaking to relative gene (SCIRR 69) of repairing damaged spinal cord separated from a mouse and the coded protein, specific antigenic epitope and transcription gene having functions during repairing damaged spinal cord and coding sequence. The function of adjusting BDNF transcription and other functions to be found; SCIRR69 protein having an important role in growth and differentiation of nerve cell and axon plasticity; specific antigen and specific antibody based on antigen preparation; drugs which can be used for preparing and promoting BDNF transcription, promoting nerve regenerating, treating nerve degeneration diseases such as nerve injuries, senile dementia and parkinsonism; drugs which can be used for preparing and repairing damaged spinal cord; agent which can be used for developing relative research and diagnosis. The invention has the advantages of clear structure and easy preparation. The invention can be used as a specific antibody of antigen preparation.

Description

technical field [0001] The invention belongs to the field of genetic engineering. In particular, it involves the SCIRR 69 gene, regulation of BDNF transcription, and its application in biomedical research and development of biotherapeutic drugs; specifically, it involves a spinal cord injury repair-related gene isolated from rats and its encoded protein, which plays a role in the process of spinal cord injury repair Transcription factors and their coding sequences. technical background [0002] The repair of spinal cord injury has become a worldwide problem to be solved urgently. In recent years, research on the molecular mechanism of spinal cord injury repair has made great progress [1-2] , These studies have laid the foundation for re-understanding spinal cord injury and providing targeted interventions. For example, people recognize the Nogo molecule by [3] For the inhibition of nerve regeneration, the corresponding antibody was designed according to its structure to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/47C07K16/18A61K38/17A61P25/00A61P25/16A61P25/28C12Q1/68G01N33/68
Inventor 刘少君马振莲刘勇阙海萍倪艳丽常超时小燕刘涛李欣景书谦
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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