Stem tip tissue rapid cultivating and seedling method of algam dendrobium nobile

A technology of dendrobium candidum and stem tips, which is applied in the field of rapid propagation of dendrobium candidum stem tips, can solve the problems of high cost and long cultivation period, and achieve the effects of reducing production costs, shortening the cultivation period, and stabilizing genetic traits

Inactive Publication Date: 2008-05-21
KUNMING UNIV OF SCI & TECH
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Embodiment 1

[0018] Select robust wild Dendrobium officinale seedlings, use their stem tips or bud tips as explants, first rinse them with running water, then sterilize them with 70% ethanol solution for 30 seconds, rinse them again with sterile water, and put the material into a bottle , and then soaked in 0.1% mercuric chloride for 6 minutes, then washed 4 times with sterile water, and inoculated in the following sterile induction medium: B 5 +KT2.0mg / L+NAA0.8mg / L+banana juice with a concentration of 10%, under the conditions of temperature 25-28°C, light intensity 1800lx, and light time 12 hours, induce culture for 55 days to obtain round bulbs ;Transfer well-grown bulbs into the following proliferation medium: B 5 +NAA2.0mg / L+KT1.0mg / L+Potatoes with a concentration of 10%, at a temperature of 25-28°C, a light intensity of 2000lx, and a light time of 13 hours, the proliferation culture was 35 days, and the proliferation coefficient was 8.5; The round bulbs were transferred to the follo...

Embodiment 2

[0020] Select robust wild Dendrobium officinale seedlings, use their stem tips or bud tips as explants, first rinse them with running water, then sterilize them with 70% ethanol solution for 55 seconds, rinse them again with sterile water, and put the material into a bottle , and then soaked in 0.1% mercuric chloride for 10 minutes, then washed 5 times with sterile water, and inoculated in the following sterile induction medium: B 5 +KT1.5mg / L+NAA0.4mg / L+banana juice with a concentration of 15%, under the conditions of temperature 25-28°C, light intensity 2000lx, and light time 12 hours, induce culture for 65 days to obtain round bulbs ;Transfer well-grown bulbs into the following proliferation medium: B 5 +NAA1.5mg / L+KT0.8mg / L+Concentration of 15% of the potato juice for proliferation culture, culture conditions: temperature 25-28 ℃, light intensity 2200lx, under the condition of 13 hours of light, culture for 45 days, proliferation coefficient 9 ; Transfer the bulbs to the ...

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Abstract

The invention provides a breeding method for fast propagation of tissue cultured a stem tip of dendrobium officinale K. Kimura et Migo, which follows the culture steps of protocorm inducing, protocorm reproducing, protocorm differentiation, seedling strengthening and rooting. After rooting, the seedlings can be transplanted after hardening. The invention shortens the cultivation period of the Dendrobium officinale K. Kimura et Migo, which is only 5 to 6 months from protocorm to rooted seedling. The invention greatly reduces production cost. And the dendrobium seedlings nurtured by the invention have stable genetic traits, and the content of polysaccharides and Dendrobium alkaloid is similar to a wild Dendrobium officinale K. Kimura et Migo.

Description

technical field [0001] The invention relates to a seedling raising method for rapid propagation of dendrobium candidum stem tip tissue culture, which belongs to the technical field of bioengineering. Background technique [0002] Dendrobium candidum (Dendrobium candidum Wall.ex Lindl) is the best product in Dendrobium and is mainly distributed in the south of the Yangtze River. and southwestern provinces. Dendrobium officinale has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, moistening lung and relieving cough, etc. Modern pharmacological studies have shown that the polysaccharide contained in Dendrobium officinale has the functions of anti-tumor, anti-aging, enhancing human immunity and dilating blood vessels. Dendrobium candidum grows on cliffs and cliffs. Because the seeds are extremely small and have no endosperm, germination under natural conditions requires symbiosis with certain fungi. Excavated and excavated, natu...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 苏有勇孔祥莹昊桢芬王督
Owner KUNMING UNIV OF SCI & TECH
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