Nucleic acid extraction device

A nucleic acid extraction device and component technology, applied in the field of nucleic acid extraction devices, can solve the problems of difficulty in meeting high-speed and large-scale nucleic acid extraction requirements, few samples to be processed, complex system structure, etc., and achieves simple structure, convenient operation, and repeated experiments. high sex effect

Active Publication Date: 2008-06-25
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many disadvantages such as toxic substances, time-consuming sample preparation, low efficiency, few samples to be processed, and cross-contamination. It is gradually difficult to meet the requirements of high-speed and large-volume nucleic acid

Method used

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  • Nucleic acid extraction device
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Embodiment Construction

[0019] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0020] Such as figure 1 As shown, the present invention includes a shaker assembly 10 , a sample holder assembly 20 , a control system 50 , and a housing 60 for mounting the shaker assembly 10 and the control system 50 .

[0021] Such as figure 2 , image 3 As shown, the oscillator assembly 10 of the present invention includes a motor 11 , an eccentric sleeve 12 , a bearing 13 , a bearing sleeve 14 , an anti-rotation sleeve 15 and a tension spring 16 . The eccentric sleeve 12 has an eccentric hole connected with the motor 11, and the top of the eccentric sleeve 12 has an integral eccentric shaft 17. One side of the center line of the eccentric sleeve 12, and keep an eccentric distance between the two, so that when the eccentric sleeve 12 rotates eccentrically under the drive of the motor 11, the other end of the center line of the eccentric sleeve...

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Abstract

The invention relates to a nucleic acid extracting device, which is characterized in that the nucleic acid extracting device comprises an oscillator component, a sample holder component, a control system and a frame; the oscillator component comprises an electric motor, an eccentric sleeve connected on the output shaft of the electric motor, a bearing connected on the eccentric shaft of the eccentric sleeve and a bearing sleeve connected on the external ring of the bearing; a threaded sleeve integrated with the bearing sleeve is arranged at the top of the bearing sleeve and a rotation stopping sleeve is arranged on the retaining plate of the electric motor; a plurality of extension springs are connected between the rotation stopping sleeve and the bearing sleeve; the sample holder component comprises a test tube stand, a plurality of sample tubes inserted and arranged on the test tube stand and a scotch yoke arranged at the top of the sample tube; the top of the scotch yoke is connected with a locking cover and the top of the locking cover is connected with a locking handle; the bottom screw rod of the locking handle passes through the locking cover, the scotch yoke and the test tube stand so as to be fixed in the threaded sleeve of the oscillator component and the scotch yoke is elastically connected with the test tube stand. The invention can be used for extracting the nucleic acids of the microorganicsms such as the bacteria (G+ bacteria, G-bacteria, mycobacterium), fungi (yeast, white candida), viruses, etc., including the extraction of the RNA.

Description

technical field [0001] The invention relates to a nucleic acid extraction device, in particular to a nucleic acid extraction device which realizes sample lysis by combining a certain reagent in a physical vibration manner. Background technique [0002] Life science is an experimental science. A large number of biological experiments and clinical diagnosis are inseparable from sample preparation. Nucleic acid extraction is widely used in sample preparation. With the development of biotechnology, the number of samples processed in clinical diagnosis and biological experiments is often very large. The traditional nucleic acid preparation method is to add the sample to be tested into a reagent containing a chemical reagent or lyase, and achieve the preparation of the nucleic acid sample through a chemical reaction. This method has many disadvantages such as toxic substances, time-consuming sample preparation, low efficiency, few samples to be processed, and cross-contamination....

Claims

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Application Information

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IPC IPC(8): C07H21/00C07H1/08
Inventor 代春喜杨华卫马清梅陈任远孙烨磊王宪华程京
Owner CAPITALBIO CORP
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