Ultrasensitive detection of prions by automated protein misfolding cyclic amplification

A prion, protein technology, applied in 0004] The present invention generally relates to pathology, a method, a composition and a device for disease, enjoying the present invention, detecting protein or prion in a sample, biochemistry and cell biology. field
CN101218510AInactive Publication Date: 2008-07-09BOARD OF RGT THE UNIV OF TEXAS SYST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BOARD OF RGT THE UNIV OF TEXAS SYST
Publication Date
2008-07-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

A highly sensitive method for detecting prions in a sample is provided. These methods can be used to diagnose prion-mediated transmissible spongiform encephalopathy, such as bovine spongiform encephalopathy, Creutzfeldt-Jakob disease, scrapie or chronic wasting disease. In particular, methods for continuous automated cyclic expansion of prions are disclosed. The method is fast and highly sensitive, making it ideal for high-throughput testing.
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Description

Background of the invention

[0001] This application claims priority to US Provisional Patent Application Serial No. 60 / 673,302, filed April 20, 2005, which is incorporated herein by reference in its entirety.

[0002] The United States Government may have certain rights in this invention pursuant to Grant No. AG024642-01 from the National Institutes of Health.

[0003] I. Field of Invention

[0004] The present invention relates generally to pathology, biochemistry and cell biology. Specifically, the present invention provides methods, compositions and devices for detecting proteins or prions in samples, including diagnosing prion-related diseases.

[0005] II. DESCRIPTION OF RELATED TECHNOLOGY

[0006] Prion diseases, also known as transmissible spongiform encephalopathies (TSE), comprise a group of fatal infectious neurodegenerative disorders that include Creutzfeldt-Jakob disease (CJD), Kuru disease, Gerstmann syndrome (GSS), Fatal familial insomnia (FFI) and sporadic f...

Claims

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