Detection method of leptospira

Abstract

The invention discloses a detecting method for leptospira, which is based on the quantitative detection technique of nucleic acid isothermal expansion, which is characterized in that a specific primer is used, and the specific area of target gene is expanded utilizing the LAMP technological platform; under the assistance of a series of quality control and internal control detection system, the leptospira is detected in molecule level, which can realize the qualitative and quantitative detection to micro sample. Compared with the prior art, the detecting method for leptospira has the advantages of decreased non-specific expansion because of isothermal expansion, good specificity, fast detection speed, high sensitivity, no need for special equipment, low cost, easy operation, easy popularization and wide application prospect, and is suitable for experimental study, clinical detection and environment detection.

Description

Technical field [0001] The invention belongs to the technical fields of clinical medicine and biochemistry and molecular biology, and specifically relates to a method for detecting Leptospira. Background technique [0002] Leptospirosis is a severe acute infectious disease with a global epidemic and a typical zoonotic disease. Many provinces and cities in my country have found sick or sick animals and animals with bacteria, and more than 60 kinds of animals have been detected. The disease mostly occurs in summer and autumn rainy seasons, the disease is rapid, and the infection method and clinical manifestations are complicated due to different serotypes of infection. [0003] The clinical symptoms of leptospirosis are complex. And lack of specific symptoms, difficult to diagnose, especially in the early stages of illness. Delays in treatment due to misdiagnosis and delayed treatment have been reported from time to time. Therefore, pathogenic detection and rapid diagnosis of Lepto...

AI Technical Summary

Problems solved by technology

However, the PCR method is easy to cause non-specific amplification in practical application, and it is also very easy to be polluted by other exogenous nucleic acids in the environment before and after the experiment, so it often causes false positive or false negative phenomena in the experimental results, which greatly limits the technology. Application in clinical testing

Although real-time fluorescence quantitative PCR has greatly improved in technology and can quantify the detection samples, its expensive equipment and high-cost detection hinder the large-scale promotion and use of this technology

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