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Method for extracting tumour-inhibiting protein from amniotic fluid of pregnant woman

A technology for amniotic fluid and pregnant women, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., and can solve the problems of extracting anti-tumor substances that have not yet been found

Inactive Publication Date: 2008-08-27
童兴龙
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  • Abstract
  • Description
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Problems solved by technology

[0003] After searching, so far no report on extracting anti-tumor substances from amniotic fluid of pregnant women has been found

Method used

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Experimental program
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Embodiment Construction

[0053] The specific method steps of this embodiment are as follows:

[0054] (1) Collect amniotic fluid:

[0055] Through clinical testing, healthy pregnant women are screened, and healthy pregnant women voluntarily request induction of labor due to family planning reasons. Strictly aseptic operation, the amniotic fluid was drawn through abdominal wall amniocentesis and collected with sterile vessels. The selected gestational age must be 19 to 21 weeks, and amniotic fluid of other gestational weeks cannot be used, because amniotic fluid of other gestational weeks not only does not contain tumor suppressor proteins, but sometimes there are proteins that promote tumor growth. This point is a technical key point;

[0056] (2) Filtration of amniotic fluid:

[0057] At -4°C, filter the amniotic fluid with filter paper under reduced pressure to filter out insoluble impurities and amniotic cells in the amniotic fluid;

[0058] (3) centrifugal sedimentation:

[0059] Put the filt...

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Abstract

The invention relates to a method for extracting tumor suppressor protein from the amniotic fluid of pregnant women, which comprises (1) collecting amniotic fluid, (2) filtering amniotic fluid, (3) centrifugally depositing, (4) first depressurizing and drying, (5) first dialysis: using the dialysis bag whose molecular weight cutoff is 10000Dalton, (6) second depressurizing and drying, (7) second dialysis: using the dialysis bag whose molecular weight cutoff is 3000Dalton, (8) third depressurizing and drying. The extraction method has the advantages that the invention can extract tumor suppressor protein from the amniotic fluid, thereby developing a new route of tumor suppressor protein, and test result proves that mice in tumor experiment can be healed, the tumor suppressor protein of the amniotic fluid of pregnant women can act the mice tumor, the protein has specific wide spectrum anti-tumor function and the invention is beneficial to express the biological function of amniotic fluid, providing the function of embryo growth information channel.

Description

technical field [0001] The invention relates to a method for extracting tumor suppressor protein from amniotic fluid of pregnant women, which belongs to the preparation method of antitumor drugs. Background technique [0002] At present, the clinically used antineoplastic drugs mainly include six categories, and their mechanisms of action are different. The first category is chemically synthesized drugs, which include cyclophosphamide, methotrexate, etc., and their mechanism of action is to directly poison tumor cells to inhibit tumors. The second category is plant extracts, such as: vincristine, which is an alkaloid extracted from Apocynaceae periwinkle; hydroxycamptothecin, which is an alkaloid extracted from camptophyllum. Their mechanism of action is to act on a certain phase of cell division, such as the M phase, to block the division and proliferation of tumors and achieve anti-tumor effects. The third category is biological agents, such as interferon, interleukin, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34
Inventor 童兴龙
Owner 童兴龙
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