Cholinesterase visual observation color matching card and manufacture method thereof

A cholinesterase, colorimetric card technology, applied in biochemical equipment and methods, microbial determination/inspection, analysis by chemical reaction of materials, etc., can solve problems such as large size, influence, inconvenience in use , to achieve the effect of small size, low cost and easy portability

Inactive Publication Date: 2008-08-27
北京望升伟业科技发展有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent No. 91229509.0 discloses a fast assay box for cholinesterase, which must use a battery, which is inconvenient to use and has a large volume. It also needs to be compared by a

Method used

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Embodiment Construction

[0006] The present invention includes a test paper strip and a standard color comparison card. The standard color comparison card includes six color blocks, the color of each color block is the same as the blood color of the corresponding cholinesterase activity, and each different color block represents the corresponding color block. The cholinesterase activity, from shallow to deep, is 0, 30%, 50%, 70%, 80%, 100%, and 0-30%. There is a reading hole in the middle of each color block, and the size of the reading hole is A drop of blood is tiled to form a circular area, and the reverse side of the color block of the standard color card is laminated, and the invention also includes a transparent thin plastic sealing bag slightly larger than the area of ​​the test strip.

[0007] The preparation method of the present invention comprises the steps:

[0008] Solution preparation: Weigh 3.5g of bromothymol blue; 6g of acetylcholine bromide, dissolve in 500ml of absolute ethanol, add...

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Abstract

The invention discloses a choline esterase visual colorimeter and a preparation method for the same, comprising a test paper wool and a standard colorimeter, wherein, the standard colorimeter comprises six color blocks; the color of each color block is the same with the color of blood with corresponding choline esterase activity; each color block represents corresponding choline esterase activity which is relatively 0 percent, 30 percent, 50 percent, 70 percent, 80 percent and 100 percent from light to heavy; an interpretation hole is arranged on the center of each color block; the size of the interpretation hole is equal to the area of a round which is formed by tiling of a drop of blood; the reverse sides of the color blocks of the standard colorimeter are provided with pressed films. The invention also comprises a transparent thin plastic sealing sack with an area which is slightly larger than that of the test paper wool. The preparation method and the use method of the invention are very simple and convenient; because a reagent strip is directly compared with the standard colorimeter and no transparent thin plate is isolated between, the detection result is more accurate; the choline esterase visual colorimeter can be used under a common daylight lamp, does not need a power source and batteries, has smaller volume, is convenient in carrying, and greatly reduces the cost.

Description

technical field [0001] The invention relates to a device capable of testing the activity of cholinesterase in human blood, in particular a measuring device capable of judging the activity of cholinesterase after organophosphorus poisoning. Background technique [0002] Organophosphorus pesticides can be easily absorbed into organisms through the respiratory tract, digestive tract, skin or mucous membranes through different states (gas state, vapor state, aerosol state, liquid state) and cause poisoning quickly. The mechanism of organophosphorus pesticide poisoning is mainly that pesticides selectively inhibit the activity of cholinesterase in the nervous system, causing a large accumulation of acetylcholine, the transmission medium of cholinergic nerves, and acting on cholinergic receptors, resulting in severe cholinergic nervous system function. disorder, and even endanger the respiratory and circulatory function and cause the poisoned person to die. The determination of c...

Claims

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Application Information

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IPC IPC(8): G01N21/78C12Q1/46
Inventor 周明非朱望李娅曹庆民赵秀丽邢玉斌魏华
Owner 北京望升伟业科技发展有限公司
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