11 results about "Choline esterase activity" patented technology

The invention discloses a synthetic carbamate compound based on 3-substituted dioxazolone as well as a preparation method and application of the synthetic carbamate compound, and belongs to the technical field of organic synthesis. The synthesis method comprises the following steps: (1) preparing 3-substituted dioxazolone from hydroximic acid with different substituent groups and phosgene under an ice bath condition; and (2) reacting the 3-substituted dioxazolone obtained in the step (1) with different phenols (alcohols) under an alkaline condition to obtain corresponding carbamate compounds. According to the method, the yield of carbamate is further improved by adjusting the conditions of alkali, equivalent weight, temperature and the like; the preparation method provided by the invention is novel and has the advantages of mild reaction conditions, few generated impurities, high yield, high safety, convenience in operation and the like; and the compound shows good cholinesterase activity in activity screening, and can be used in insecticides or pesticides.

The invention provides a cholinesterase application method based on nano-gold stars. The cholinesterase application method comprises the following steps: synthesis and characterization of a nano-gold star material, morphology regulation and control of the nano-gold star material, and application of nano-gold star etching inhibition to cholinesterase activity detection. The embodiment of the invention provides a simple, stable and sensitive nano-gold star material morphology control method, the method is applied to cholinesterase activity detection and cholinesterase inhibitor and revivifier screening, and the method has the advantages of rapidness, low cost, ultrasensitivity, quantitative colorimetric reading and the like.

The invention discloses a compound which has anti-neuroinflammation activity and can selectively inhibit butyrylcholine esterase activity, and belongs to the technical field of medicinal chemistry. The compound and the pharmaceutical composition containing the compound have good anti-neuroinflammatory activity and selective butyrylcholine esterase inhibition activity, and have important application prospects in treatment of neurodegenerative diseases such as Alzheimer's disease.

The invention belongs to the technical field of biological peptide enzymolysis, and particularly discloses a sheep three-medullary peptide composition for assisting in improving memory and application of the sheep three-medullary peptide composition. The sheep three-medullary peptide composition is prepared from the following components in parts by mass: 40 to 50 parts of sheep three-medullary peptide, 20 to 35 parts of sheep colostrum peptide and 10 to 25 parts of casein hydrolyzed peptide. The invention discloses a sheep three-medullary peptide composition for assisting in improving memory and application thereof, which can obviously improve the level of acetylcholine in the brain and inhibit the activity of acetylcholin esterase to enhance nerve conduction, and can effectively relieve the damage of oxidative stress to brain tissues by improving the activity of SOD and GSH-Px and reducing the content of MDA.

The application discloses a kind of Ascophyllum nodosum fucoidan ANFS and its preparation method and application in preparation for preventing and treating alzheimer's disease preparation, belong to marine biological medicine technical field.The main chain of the Ascophyllum nodosum fucoidan ANFS is composed of (1→3) connected α-L fucose residue, and branch is monosaccharide or sulfate group.Sulfated modification occurs at C-2 and C-4 of fucose residue, and its molecular weight is 15-30 kDa.The application proves by experiment, ANFS can significantly improve the motion behavior defect of zebra fish, inhibit acetylcholinesterase activity, improve catalase and glutathione peroxidase activity, reduce intracellular reactive oxygen species level, reduce brain neuron apoptosis, and effectively protect the integrity of blood-brain barrier.The polysaccharide is rich in source, and preparation process is simple, and safety is high, has wide application prospect in the development of marine medicine and functional product for treating alzheimer's disease.

This invention discloses the use of cyanidin anthocyanins or their pharmaceutically acceptable salts, esters, and solvates in the preparation of drugs for the prevention and / or treatment of neurodegenerative diseases. The cyanidin anthocyanins are selected from cyanidin-3-O-rutin glycoside, cyanidin-3-O-sophoroside, or cyanidin-3-O-xyloside. These compounds can inhibit acetylcholinesterase activity, upregulate α7 nicotinic acetylcholine receptor expression, and activate the PI3K / AKT / GSK3β signaling pathway, thereby inhibiting Tau protein hyperphosphorylation and reducing neuroinflammatory responses. Furthermore, it has been confirmed that cyanidin-3-O-rutin glycoside directly binds to acetylcholinesterase, verifying AChE as its key target, providing new natural active compounds and their mechanisms of action for the prevention and treatment of neurodegenerative diseases.

The application belongs to the technical field of medicine, and particularly relates to a steroidal compound in medicinal fungus Shandong ganoderma, a preparation method of the steroidal compound and application of the steroidal compound in resisting Alzheimer's disease. The structures of the steroidal compounds 1-4 are shown in the following; the application further provides application of the compounds in preparing medicines for resisting Alzheimer's disease. The preparation method is simple, reproducible, the obtained compound has high purity, and has good inhibitory activity on acetylcholinesterase.

The present application relates to the field of microorganisms, and provides a propionibacterium acidifaciens BL8 with functions of reducing blood sugar and resisting aging, and application thereof, the propionibacterium acidifaciens BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No.33134.The propionibacterium acidifaciens BL8 of the present application can inhibit DPP4 activity, promote INS-1 insulin secretion, inhibit acetylcholinesterase activity and superior cellulase activity.Can reduce blood sugar;Improve memory, prevent and relieve Alzheimer's disease;Promote cellulose absorption, improve constipation, and have high research and application value in reducing blood sugar and resisting aging.

The invention relates to the technical field of cannabidiol derivatives, and discloses a cannabidiol amino acid derivative and a preparation method and application thereof.The preparation method comprises the steps that in the inert atmosphere, cannabidiol and a dichloromethane solvent are mixed, DMAP and EDCI are added after the cannabidiol is completely dissolved, stirring is conducted for 10 min, an amino acid compound is added, stirring reaction is continuously conducted for 24 h at the room temperature, and the cannabidiol amino acid derivative is obtained; pouring the reaction liquid into a separating funnel, performing liquid extraction by using dichloromethane and water, retaining an organic phase, washing with water, then drying and filtering the organic phase by using anhydrous sodium sulfate, concentrating the filtrate under reduced pressure to constant weight to obtain a reaction crude product, and finally separating and purifying the reaction crude product by using silica gel column chromatography. The whole synthesis steps are simple, and the prepared cannabidiol amino acid derivative has the effects of remarkably inhibiting the activity of tyrosinase and cholinesterase and also has a certain nerve protection effect.

The present application belongs to the technical field of pharmaceutical compositions, and particularly relates to a composition containing yak milk fat globule membrane and capable of improving cognitive function decline and application thereof. The composition takes yak milk fat globule membrane as a core active ingredient, and takes taurine, selenium-rich yeast and yam extract as auxiliary active ingredients. Through synergistic effect of the components, the composition can target inhibit acetylcholinesterase activity, relieve oxidative stress damage of brain nerve cells, reduce the level of pro-inflammatory factors in the brain, and then significantly improve cognitive function. The composition takes into account functionality and nutrition, and shows broad application prospects in the development of products related to improving learning and memory ability, delaying cognitive function decline and improving cognitive impairment.

The invention discloses ascophyllum nodosum fucoidin ANFS, a preparation method thereof and application of the ascophyllum nodosum fucoidin ANFS in preparation of a preparation for preventing and treating Alzheimer's disease, and belongs to the technical field of marine biological medicine. The main chain of the ascophyllum nodosum fucoidin ANFS is composed of alpha-L fucoidin residues connected with (1-> 3), and the branch chain is monosaccharide or sulfate group. Sulfating modification occurs at C-2 and C-4 sites of fucose residues, and the molecular weight of the fucose residues is 15-30 kDa. Experiments prove that ANFS can remarkably improve the movement behavior defect of zebrafish, inhibit the activity of acetylcholin esterase, improve the activity of catalase and glutathione peroxidase, reduce the level of active oxygen in cells, reduce brain neuron apoptosis and effectively protect the integrity of a blood brain barrier. The polysaccharide is rich in source, simple and convenient in preparation process and high in safety, and has a wide application prospect in the aspect of developing marine drugs and functional products for treating the Alzheimer's disease.