Herba argyreiae acutae alkali as well as extraction method and purpose thereof

An extraction method and a technique for sphenoidine, which are applied in the field of sagebrush extracts, can solve the problems of restricting the development and application of medicinal preparations of sagebrush, unclear active ingredients, weak research on the basis of medicinal materials and mechanisms of action, and the like. The method is simple and easy to implement, has good anti-inflammatory effect, and has excellent inhibitory effect on acetylcholinesterase activity

Inactive Publication Date: 2017-12-26
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although Baiheteng is commonly used in Guangxi to treat inflammation, cough and other diseases, and has also been included in the "Guangxi Zhuang Autonomous Region Zhuang Medicine Quality Stand...

Method used

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  • Herba argyreiae acutae alkali as well as extraction method and purpose thereof
  • Herba argyreiae acutae alkali as well as extraction method and purpose thereof
  • Herba argyreiae acutae alkali as well as extraction method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The extraction method of serratine, it comprises the following steps:

[0044] 1) the white crane vine is ground into powder, add ethanol solvent, adopt cold dipping method to extract, evaporate ethanol solvent, obtain the first extract;

[0045] 2) The first extract is mixed with water to form a suspension, and petroleum ether, ethyl acetate and n-butanol are successively added to the suspension for extraction, the n-butanol extract is collected, and after concentrating, the second extract is obtained. paste;

[0046] 3) Take the second extract, carry out macroporous resin column chromatographic separation, use water-ethanol mixed solution as eluent, carry out gradient elution, and collect the eluate whose mass fraction of ethanol is 40% to 70% , the eluate is concentrated to obtain the third extract;

[0047] 4) The third extract is recrystallized from methanol to obtain the cymenine.

Embodiment 2

[0049] The extraction method of serratine, it comprises the following steps:

[0050] 1) Grinding the white crane vine into powder, adding a volume fraction of 75% ethanol solvent of 15 times the weight of the white crane vine coarse powder, soaking at 0° C. for 24 hours to extract the white crane vine, evaporating the ethanol solvent to obtain the first extract;

[0051] 2) The first extract is mixed with water to form a suspension, and petroleum ether, ethyl acetate and n-butanol are successively added to the suspension for extraction, the n-butanol extract is collected, and after concentrating, the second extract is obtained. paste;

[0052] 3) Take the second extract, carry out macroporous resin column chromatographic separation, use water-ethanol as the eluent, carry out gradient elution, collect the eluent with an ethanol mass fraction of 40% to 70%, and use The eluate is concentrated to obtain the third extract;

[0053] 4) The third extract is recrystallized from met...

Embodiment 3

[0055] The extraction method of serratine, it comprises the following steps:

[0056] 1) Grind the white crane vine into powder, add 10 times the weight of the white crane vine coarse powder and the volume fraction of 95% ethanol solvent, soak it at 8 ° C for 6 hours to extract the white crane vine, use a rotary evaporator to evaporate the ethanol solvent, and the evaporation temperature is 40 ℃, the vacuum degree is 0.022MPa, and the first extract is obtained;

[0057] 2) The first extract is mixed with water to form a suspension, and petroleum ether, ethyl acetate and n-butanol are successively added to the suspension for extraction, the n-butanol extract is collected, and after concentrating, the second extract is obtained. paste;

[0058] 3) Take the second extract, carry out macroporous resin column chromatographic separation, use water-ethanol as the eluent, carry out gradient elution, collect the eluent with an ethanol mass fraction of 40% to 70%, and use The eluate i...

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Abstract

The invention discloses herba argyreiae acutae alkali as well as an extraction method and a purpose thereof. The extraction method of the herba argyreiae acutae alkali comprises the following steps of (1) grinding herba argyreiae acutae into powder; adding an ethanol solvent; using a cold soaking method for extraction; evaporating an ethanol solvent to obtain a first extract; (2) adding water into the first extract to form suspension liquid; sequentially adding petroleum ether, ethyl acetate and n-butyl alcohol for extraction; collecting n-butyl alcohol extraction liquid; concentrating the n-butyl alcohol extraction liquid into an extract to obtain a second extract; (3) performing macroporous resin column chromatography separation for the second extract; performing gradient elution with water-ethanol as an eluent; collecting the elution solution with the ethanol mass percentage being 40 to 70 percent; concentrating the elution solution to obtain a third extract; (4) performing methanol recrystallization on the third extract; obtaining a target product of the herba argyreiae acutae alkali. The extraction method is simple and convenient; the implementation is easy; the extraction rate is high; the compound has good anti-inflammatory effects and excellent acetylcholinesterase activity inhibition effects.

Description

technical field [0001] The present invention relates to the technical field of extracts of sagebrush, and more specifically, the present invention relates to a sagebine and an extraction method and uses thereof. Background technique [0002] White Crane Vine (Argyreia acuta Lour.) belongs to the Convolvulus family and belongs to the genus Silverback. Zhuang medicine is clinically used to treat various inflammation-related diseases such as cough, expectoration, hemostasis, bruises, boils, etc. "Chinese Zhuang Pharmacy" records that Baihe vine is used for acute and chronic bronchitis, cough, bruises, etc. Residents in Dehong Prefecture, Yunnan like to eat it as a wild vegetable because it is fresh, smooth, odorless, and has a certain medicinal effect. White Crane vine has been included in the "Quality Standards for Zhuang Medicines in Guangxi Zhuang Autonomous Region". The medicinal materials are rich in resources and easy to grow. They are widely distributed in Guangxi, Guan...

Claims

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Application Information

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IPC IPC(8): C07H15/26C07H1/08A61P29/00A61P25/28
CPCC07H1/08C07H15/26
Inventor 卢汝梅韦建华李兵朱小勇蒙秋艳赵惠玲
Owner GUANGXI UNIV OF CHINESE MEDICINE
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