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Method for simultaneously determining of concentration multi anesthesia medicament in blood plasma

A technology of local anesthesia and drug concentration, which is applied in the field of medical testing to reduce analysis costs, simplify analysis steps, and improve detection sensitivity

Inactive Publication Date: 2009-03-25
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no literature report on the relevant analytical methods for the simultaneous determination of the above five drugs at home and abroad.

Method used

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  • Method for simultaneously determining of concentration multi anesthesia medicament in blood plasma
  • Method for simultaneously determining of concentration multi anesthesia medicament in blood plasma
  • Method for simultaneously determining of concentration multi anesthesia medicament in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Chromatographic conditions

[0031] Waters 2690 HPLC system, Waters 2487 dual-wavelength UV detector, Millennium32 chromatographic workstation (Version 4.0); chromatographic column: Kromasil C 18 (250mm×4.6mm, 5μm); Column temperature: 40°C; Mobile phase: 30mmol / L potassium dihydrogen phosphate aqueous solution (containing 0.14% triethylamine solution, phosphoric acid solution to adjust pH=4.8): acetonitrile (61:39, V / V); flow rate 1.0mL min -1 ; The concentration of LID, ROP and BUP was measured at 210nm, and the concentration of PRO and TET was measured at 290nm.

[0032] Plasma sample pretreatment

[0033] Take 0.5mL blood sample and add 50μl of 0.5mg / mL neostigmine solution, 100μl of 5μg / mL internal standard solution and 100μL of 0.5mol / L KaOH solution, vortex for 10Sec, mix well, add 3mL of diethyl ether, all the above operations are at 3℃ Carry out under the following ice bath conditions; vortex for 2min, centrifuge at 2500g×8min, take 2.5mL of supernatant in ...

Embodiment 2

[0045] Chromatographic conditions

[0046] Waters 2690 HPLC system, Waters 2487 dual-wavelength UV detector, Millennium32 chromatographic workstation (Version 4.0); chromatographic column: Kromasil C 18 (250mm×4.6mm, 5μm); Column temperature: 40°C; Mobile phase: 30mmol / L potassium dihydrogen phosphate aqueous solution (containing 0.18% triethylamine solution, phosphoric acid solution to adjust pH=5.1): acetonitrile (65:35, V / V); flow rate 1.0mL min -1 ; The concentration of LID, ROP and BUP was measured at 210nm, and the concentration of PRO and TET was measured at 290nm.

[0047] Plasma sample pretreatment

[0048] Take 0.5mL blood sample and add 50μl of 2mg / mL neostigmine solution, 100μl of 5μg / mL internal standard solution and 100μL of 2mol / L NaOH solution, vortex for 8Sec, mix well, add 3mL of diethyl ether, the above operations are all under 3°C on ice Carry out under bath conditions; vortex for 2min, centrifuge at 2500g×10min, take 2.5mL supernatant in another test t...

Embodiment 3

[0059] Chromatographic conditions

[0060] Waters 2690 HPLC system, Waters 2487 dual-wavelength UV detector, Millennium32 chromatographic workstation (Version 4.0); chromatographic column: Kromasil C 18 (250mm×4.6mm, 5μm); Column temperature: 40°C; Mobile phase: 30mmol / L potassium dihydrogen phosphate aqueous solution (containing 0.16% triethylamine solution, phosphoric acid solution to adjust pH=4.9): acetonitrile (63:37, V / V); flow rate 1.0mL min -1 ; The concentration of LID, ROP and BUP was measured at 210nm, and the concentration of PRO and TET was measured at 290nm.

[0061] Plasma sample pretreatment

[0062] Take 0.5mL blood sample and add 50μl of 1mg / mL neostigmine solution, 100μl of 5μg / mL internal standard solution and 100μL of 1mol / L NaOH solution, vortex for 12Sec, mix well, add 3mL of diethyl ether, the above operations are all under 3°C on ice Under bath conditions; vortex for 2min, centrifuge at 3000g×9min, take 2.5mL supernatant in another test tube, blow ...

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Abstract

The invention belongs to the field of medical examination and relates to a method capable of synchronously determining the concentrations of a plurality of local anesthetic drugs in human plasma. The method adopts a plasma cholineesterase inhibitor to inhibit the activity of plasma cholinesterase under ice bath condition below 3 DEG C, which controls the hydrolysis of totokaine and assures the accuracy of the method; by utilizing characteristics that lidocaine, ropivacaine and bupivacaine have stronger characteristic of ultraviolet absorption at wavelength of 210nm, and procaine and the totokaine have stronger characteristic of ultraviolet absorption at wavelength of 290nm, an ultraviolet dual-wavelength method is used to detect after the separation of an acid mobile phase at a chromatographic column; and the method can ensure that the sensitivity of synchronous determination of the local anesthetic drugs is greatly improved. The method has less sampling from samples and simple, quickand sensitive pretreatment, does not need expensive equipment or reagents, has short analysis period and low cost, and is suitable for the monitoring of clinical conventional blood concentration of aplurality of drugs.

Description

technical field [0001] The invention belongs to the field of medical examination and relates to an analysis and determination method of drugs in vivo, in particular to a method for simultaneously measuring the concentrations of multiple local anesthetic drugs in human blood plasma. Background technique [0002] Procaine (PRO), lidocaine (LID), ropivacaine (ROP), tetracaine (TET) and bupivacaine (BUP) are currently commonly used local anesthetics in clinical practice. Among them, ROP and BUP are long-acting local anesthetics with slow onset but long acting time, while PRO, TET, and LID are short-acting local anesthetics with fast onset but short acting time. Clinically, long-acting and short-acting local anesthetics are often combined to facilitate the smooth implementation of the operation. The pharmacokinetics of the five drugs PRO, LID, ROP, TET and BUP vary greatly among individuals, combined medication is common, abnormal liver and kidney function will lead to changes i...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N30/02G01N21/17
Inventor 覃韦苇焦正钟明康施孝金李中东崔学艳
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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