Use of medicament of geldanamycin derivative for preparing medicament for curing chemotherapy induction alopecia

[0030] Example: Animal model and treatment of hair loss after chemotherapy

[0031] 1. Experimental materials

[0032] The experimental animals were SPF Km mice aged 4-6 weeks, provided by the Experimental Animal Center of Sun Yat-Sen University, and raised at room temperature.

[0033] Cyclophosphamide and paclitaxel for injection were purchased from Sun Yat-sen University Cancer Hospital, and other commercially available products can also be used;

[0034] Therapeutic drugs: 17-propenylamino-17-demethoxygeldanamycin (17-AAG), purchased from SIGMA.

[0035] Others: physiological saline; chloral hydrate; heating equipment such as water bath.

[0036] 2. Experimental methods and results

[0037] The experimental mice were randomly divided into five groups: control group, cyclophosphamide chemotherapy experimental group (no drug treatment), cyclophosphamide chemotherapy + drug treatment group, paclitaxel chemotherapy group (no drug treatment), paclitaxel chemotherapy + drug treatment group.

[0038] Cut off the back hairs of the mice in each of the above groups, and after the new hairs grow out, inject cyclophosphamide 120mg/kg body weight and paclitaxel 50mg/kg body weight into the abdominal cavity to induce hair loss (except the control group), and inject it every seven days One time, three consecutive injections.

[0039] Results: Compared with the control group, the mice in the cyclophosphamide and paclitaxel chemotherapy group showed obvious hair loss. Such as figure 1 Shown.

[0040] The mice were treated after the third injection. Cut off all the remaining hairs on the back of the mouse, and inject 0.05ml of 50μM drug (geldanamycin 17-AAG) intracutaneously into the back. The treatment area is about 7-8cm. 2. Histochemical HE staining was used to detect hair follicle changes.

[0041] Result: After a week, new hairs grew on the back and around the area where Geldanamycin 17-AAG was injected, while the other parts were not long and formed round spots. The effect was obvious. figure 2 Shown.

[0042] The weight change of the drug before and after administration can be used to analyze the toxic and side effects of the drug on mice. After testing, the average weight data of each group of mice is shown in Table 1 and image 3 Shown:

[0043] Table 1: Changes in body weight of mice in each group before and after treatment

[0044] Normal control mice

[0045] Conclusion: The drug has no obvious side effects on mice.

[0046] The mice in each group were put to death by pulling their tails and necks severed. The skin tissues of the back were stained with histochemical HE staining method to observe the changes of hair follicles. Such as image 3 As shown, a is the normal control group, and the number of hair follicles is normal; b is the treatment group after paclitaxel chemotherapy, and the number of hair follicles is the same as the normal group; c is the treatment after cyclophosphamide chemotherapy, and the number of hair follicles is the same as the normal group; d is the non-treatment group of cyclophosphamide chemotherapy, with fewer hair follicles; e is the non-treatment group of paclitaxel chemotherapy, with fewer hair follicles.