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Dilution for preserving semen under normal temperature

A technology of preservation and dilution at room temperature, applied in the field of diluent of semen in the improvement of artificial insemination technology, can solve the problems of short sperm motility preservation time, difficult to popularize and apply on a large scale, restricting the development of aquaculture industry, etc. The effect of promoting application and improving sperm motility

Inactive Publication Date: 2008-09-17
高庆华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of semen diluent at room temperature at home and abroad has a short preservation time for sperm motility, unsatisfactory preservation effect, and unstable conception effect, which is difficult to promote and apply on a large scale, which seriously restricts the development of my country's breeding industry.

Method used

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  • Dilution for preserving semen under normal temperature
  • Dilution for preserving semen under normal temperature
  • Dilution for preserving semen under normal temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Mix 120ml of double distilled water, 1.0g of fructose, 4.5g of Tris, 2.0g of citric acid, 30mg of GSH, 150mg of BSA, 35mg of ATP, 450mg of VC, 300,000 penicillin, 200,000 streptomycin and 1.0g trehalose.

Embodiment 2

[0025] Mix 136ml of double distilled water, 2g of fructose, 4.84g of Tris, 2.68g of citric acid, 40mg of GSH, 160mg of BSA, 40mg of ATP, 500mg of VC, 400,000 penicillin, 200,000 streptomycin and 1.5g trehalose.

Embodiment 3

[0027] Mix 140ml of double distilled water, 3.0g of fructose, 5.5g of Tris, 3.5g of citric acid, 30mg of GSH, 180mg of BSA, 50mg of ATP, 550mg of VC, 500,000 penicillin, 250,000 streptomycin and 2.0g trehalose.

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Abstract

The invention discloses a normal temperature preservation diluent for sperm, comprising 120ml to 140ml double distilled water, 1.0g to 3.0g fructose, 3.5g to 5.5g Tris, 2.0g to 3.5g citric acid, 30mg to 50mg GSH, 140mg to 180mg BS, 30mg to 50mg ATP, 450mg to 550mg VC, 300,000 to 500,000 penicillin, 150,000 to 250,000 streptomycin and 1.0g to 2.0g trehalose; the optimum operating temperature ranges from 15 DEG C to 25 DEG C. The normal temperature preservation diluent for sperm has the advantages that, the diluent has good effect on the normal temperature preservation of the sperm of cattle, sheep and deer; the invention has simple production method, low production cost and convenient popularization and application.

Description

technical field [0001] The invention relates to a diluent of semen in the improvement of artificial insemination technology, in particular to a diluent suitable for storing semen of cattle, sheep and deer at room temperature. Background technique [0002] With the continuous development of my country's breeding industry, the rapid popularization of artificial insemination, artificial insemination stations of different scales have become an important part of my country's breeding industry. In order to make full use of excellent male breeds, give full play to the advantages of their male breeds, and achieve real resource sharing, artificial insemination stations have semen storage time and semen storage effect that are the key factors for the success of artificial insemination applications. The diluent which is good and whose cost is significantly lower than the existing commercial semen diluent has important economic benefits for artificial insemination technology. At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 高庆华
Owner 高庆华