Inoculation method of seed cells for constructing tissue engineering nucleus pulposus of intervertebral disc

Abstract

The invention provides a seeded cell inoculation method for use in a tissue engineering of constructing intervertebral disc nucleus pulposus. A transparent hyaluronic acid is used as a carrier for the inoculation of seeded cells. The inoculation method comprises the following steps of: human or animal suspending intervertebral disc nucleus pulposus cells with the transparent hyaluronic acid; impregnating a supporting material with culture solution and absorbing the water for later use; dripping cell suspension slowly to the supporting material and keeping the supporting material stand; removing the cell-supporting material combined body into a culture flask; placing the culture solution slowly into the flask; the cell can be introduced into an animal or human body for research or treatment when the cells proliferate in the supporting material and extracellular matrixes are synthesized. The method has the advantages of improving the biological performance of the constructed tissue, the hesion rate of the seeded cells and the distribution uniformity of the seeded cells in the supporting material, along with safety, biomimetic effect, etc.

Description

A seed cell inoculation method applied to the construction of tissue engineering intervertebral disc nucleus pulposus technical field The invention belongs to the construction technology of tissue engineering intervertebral disc nucleus pulposus, in particular to a seed cell inoculation method applied to the construction of tissue engineering intervertebral disc nucleus pulposus. Background technique In the process of constructing the tissue-engineered intervertebral disc nucleus pulposus, the inoculation of seed cells is a key technology. The choice of inoculation method directly affects the distribution of seed cells on the scaffold material, the shelf rate and the biological activity of cells, which in turn determines the quality of the constructed tissue. biological function. At present, there are many inoculation methods of seed cells reported in literature at home and abroad, among which the static method is the most commonly used, which is simple and easy to impleme...

AI Technical Summary

Problems solved by technology

At present, there are many inoculation methods of seed cells reported in literature at home and abroad, among which the static method is the most commonly used method, which is simple and easy to implement, but the biggest problem with this method is that the rate of cell placement is very low, and the inoculated cells are in the scaffold material. uneven distribution of

So the researchers tried to inoculate with dynamic inoculation, including perfusion seeding (perfusion inoculation), spinner flask seeding (rotary inoculation), agitated seeding (agitated inoculation), etc., but the efficiency of these methods is still controversial, and requires special help. device of

In addition, other inoculation methods, such as oscillating perfusion (oscillating inoculation method), filtration seeding (filter inoculation method) and other operations are more complicated, and are mostly limited by the shape of the material