Delta6 fatty acid desaturated enzyme of marine microalgae and applications thereof

Abstract

The invention provides marine microalgae delta6-fatty acid desaturase, and coding genes and application thereof. The delta6-fatty acid desaturase is provided with amino acid sequences shown in a sequence table SEQ ID NO.2 or amino acid sequences which are formed by replacement, shortage or addition of one amino acid residue or a plurality of amino acid residues and have the same functions. The delta6-fatty acid desaturase can effectively catalyze Linoleic Acid (LA, 18:2delta9, 12) to generate gamma- Linolenic Acid (GLA, 18:3delta6, 9, 12) and catalyze alpha-Linolenic Acid (ALA, 18:3delta9, 12, 15) to generate Stearidnoic Acid (SDA, 18:4delta6, 9, 12, 15).

Description

Marine microalgae delta6 fatty acid desaturase and application thereof Technical field The invention belongs to the field of biotechnology, and specifically relates to a Δ6 fatty acid desaturase of marine microalgae-Pseudochlorococcum, its coding gene and application. Background technique Fatty acids are monocarboxylic acids with hydrocarbon chains, which play an important role in many biological processes. Fatty acids are less in free form, but are lipidated into various major lipid components, such as phospholipids and triacylglycerols. Fatty acids are mainly divided into two categories: saturated fatty acids and unsaturated fatty acids. The latter is further divided into monounsaturated fatty acids and polyunsaturated fatty acids, each of which contains one or more cis double bonds (C=C) in the hydrocarbon chain. The formation of unsaturated bonds requires fatty acid desaturase (fattyacid desaturase) catalysis. Fatty acid desaturases exist in almost all plants, animals and m...

AI Technical Summary

Problems solved by technology

However, there is no relevant report about the Δ6 desaturase of Nannochloropsis sp.

Images