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Process of fly louse with noxious ash by using freezing method

A technology of SBPH and virus, which is applied in the field of plant disease resistance identification, can solve the problems of cumbersome and complicated operation, and achieve the effects of speeding up speed and accuracy, rapid development, speeding up identification speed and selection process

Inactive Publication Date: 2010-06-30
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the identification results of indoor inoculation identification at the seedling stage are stable and reliable, and it is the preferred method for resistance identification. However, due to the strong mobility of the transmission vector SBPH, quantitative inoculation of SBPH has always been a complicated and cumbersome operation. Work

Method used

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  • Process of fly louse with noxious ash by using freezing method
  • Process of fly louse with noxious ash by using freezing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1, the freezing time gradient experiment of poisonous striatellus striatellus

[0011] Take 6 dry glass cups, number them, and select a certain percentage of SBPH carrying rice stripe virus to put in them, about 100 worms per cup. At the same time, put them in the refrigerator at -20°C and start freezing and timing. Take out 1 cup every 10 minutes, quickly pour the planthoppers on a white paper after taking them out, count them with a brush, observe and record the active state of the planthoppers, the time of awakening and the number of dead insects. The frozen SBPH were reared separately with Wuyujing 3, and the number of dead insects was counted on the second day.

[0012] The results showed that: after freezing for 10 minutes, SBPH would not be comatose, and could still jump, which was not conducive to counting insects on paper; however, as the freezing time exceeded 30 minutes, the vitality of SBPH would be affected, and the mortality rate increased with...

Embodiment 2

[0015] Embodiment 2, the test of the influence of freezing treatment on the transmission ability of poisonous striatellus striatellus

[0016] Soak the seeds of Wuyujing No. 3, accelerate the germination, and sow them in glass cups when they reach 0.5 leaves; 12 seedlings are sown in each cup, and 6 cups are sown. Take about 120 head from the group of SBPH carrying rice stripe virus in a certain proportion, freeze SBPH at -20°C for 20 minutes according to the above method, count 120 worms, and inoculate them on Wuyujing No. 3 with 1.5 leaf age , repeat 3 times. At the same time, 120 worms were inoculated onto Wuyujing No. 3 with a leaf age of 1.5 with a straw, and repeated 3 times. The glass was sealed with gauze, and after 48 hours, the SBPH were removed. Transplanted in the field, pay attention to field management. After 7 days, start to investigate the disease of the plants (refer to the content of the invention for the classification standard of the disease grade), and ...

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Abstract

The invention discloses a novel method for treating small brown rice planthopper which carries virus. The essence of the invention leads the moving ability of the small brown rice planthopper to drop temporarily by adopting a refrigeration method so as to start the operation of quantitative capturing. With the recovery of body temperature, the abilities of moving, feeding and virus transmitting of small brown rice planthopper are recovered again; therefore, the purpose for moving small brown rice planthopper rapidly and quantitatively can be achieved. Compared with the original method, the method of the invention is characterized by being more convenient and rapid and is applied to inoculation identification of small brown rice planthopper which transmits virus diseases such as stripe virus of rice, etc.

Description

technical field [0001] The invention relates to a method for treating virus-carrying striatellus striatellus, and belongs to the technical field of identification of plant disease resistance. Background technique [0002] Rice stripe blight caused by rice stripe virus (RSV) was first discovered in Kanto, Japan in 1897, and later occurred in Korea, Ukraine and China. Since the virus was discovered in Jiangsu and Zhejiang in 1962 in my country, it has occurred in 16 provinces, municipalities, and autonomous regions across the country. At present, the rice fields in the lower reaches of the Yangtze River, mainly in Jiangsu Province, are still in the epidemic period. According to incomplete statistics, the occurrence area of ​​rice stripe blight in Jiangsu Province reached 27 million mu in 2004 alone, accounting for 79% of the rice area in Jiangsu Province. Experts predict that rice stripe blight will still show a tendency to occur more heavily in the five years after 2006 , pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01H1/04
Inventor 周彤周益军陈洁程兆榜季英华熊如意
Owner JIANGSU ACAD OF AGRI SCI